| 张 利,姚俊芳,张 磊.CTSB在肝内胆管细胞癌中表达及对癌细胞增殖及NF-κB信号通路的影响[J].肿瘤学杂志,2020,26(10):886-890. |
| CTSB在肝内胆管细胞癌中表达及对癌细胞增殖及NF-κB信号通路的影响 |
| Expression of CTSB in Intrahepatic Cholangiocarcinoma and Its Effect on Proliferation of Cancer Cells and NF-κB Signaling Pathway |
| 投稿时间：2019-11-22 |
| DOI：10.11735/j.issn.1671-170X.2020.10.B007 |
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| 中文关键词: 人组织蛋白酶B 肝内胆管细胞癌 核因子-κB信号通路 细胞增殖 |
| 英文关键词: |
| 基金项目:河南省医学科技攻关计划项目（2018020772） |
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| 中文摘要: |
| 摘 要：［目的］探讨人组织蛋白酶B（CTSB）在肝内胆管细胞癌中表达及对癌细胞增殖及核因子-κB（NF-κB）信号通路的影响。［方法］ 采用Western blot检测肝内胆管癌细胞中CTSB蛋白表达，使用siRNA对CTSB沉默后采用Edu实验检测细胞增殖，同时检测NF-κB信号通路中相应蛋白表达情况。［结果］与正常肝细胞系相比，肝内胆管癌细胞RBE、HCCC9810、HUCCT1中CTSB蛋白表达水平均明显提高（t=7.724、8.839、7.983，P=0.002、0.001、0.002）。与Control组相比，CTSB-siRNA组的CTSB蛋白表达量明显下降（t=6.131，P=0.004），细胞增殖比例明显下降（t=5.271，P=0.006），且CTSB-siRNA组的NF-κB抑制蛋白α（IκB-α）、IκB激酶β（IKK-β）、IKKα和p-NF-κB蛋白水平明显下降（t=6.069、5.433、6.365、5.717，P=0.004、0.006、0.003、0.005）。［结论］ 肝内胆管细胞癌中CTSB蛋白表达水平明显上调，且CTSB能够通过活化NF-κB信号通路促进肿瘤细胞增殖。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the expression of CTSB in intrahepatic cholangiocarcinoma and its effect on the proliferation of cancer cells and the NF-κB signaling pathway.［Methods］ The expression of CTSB protein in intrahepatic bile duct cancer cells was detected by Western blot. After silencing CTSB with siRNA，cell proliferation was detected by Edu assay and corresponding protein expression in NF-κB signaling pathway was detected.［Results］ Compared with normal liver cell lines，the expression level of CTSB protein in intrahepatic bile duct cancer cells(RBE，HCCC9810 and HUCCT1) was significantly increased(t=7.724，8.839 and 7.983，P=0.002，0.001 and 0.002，respectively).Compared with the control group，the expression of CTSB protein in the CTSB-siRNA group decreased significantly(t=6.131，P=0.004). Compared with the Control group，cell proliferation in the CTSB-siRNA group was significantly decreased(t=5.271，P=0.006). Compared with the control group，the expression levels of IKKβ，IKKα，p-IKBα and p-NF-Κb proteins in the CTSB-siRNA group decreased significantly(t=6.069，5.433，6.365 and 5.717，P=0.004， 0.006，0.003 and 0.005，respectively).［Conclusion］ The expression level of CTSB protein in intrahepatic cholangiocarcinoma is significantly up-regulated，and CTSB can promote the proliferation of tumor cells by activating NF-κB signaling pathway. |
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