| 陈涵波,田晓康,宗 丹.ZNF488对鼻咽癌细胞放疗敏感性的影响[J].肿瘤学杂志,2020,26(10):869-874. |
| ZNF488对鼻咽癌细胞放疗敏感性的影响 |
| Effect of ZNF488 on Radiosensitivity of Nasopharyngeal Carcinoma Cells |
| 投稿时间：2020-06-08 |
| DOI：10.11735/j.issn.1671-170X.2020.10.B004 |
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| 中文关键词: 鼻咽肿瘤 ZNF488 放射敏感性 |
| 英文关键词:nasopharyngeal carcinoma ZNF488 radiosensitivity |
| 基金项目:国家青年科学基金项目（81702693）；医院科研基金项目（ZK201602） |
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| 中文摘要: |
| 摘 要：［目的］ 探究沉默ZNF488后鼻咽癌细胞的放射敏感性变化。［方法］ 基于GEPIA和UALCAN数据平台，检索ZNF488在头颈鳞状细胞癌中的数据信息。在鼻咽癌细胞系中沉默ZNF488，采用qRT-PCR 和 Western blot 检测 ZNF488 siRNA转染效率。在剂量梯度的电离辐射处理下，进行克隆形成和CCK-8检测，评估电离辐射对鼻咽癌细胞增殖能力和存活率的影响。［结果］ 基于生信数据平台，检测到ZNF488在头颈部鳞癌中高表达，与预后较差相关，且与MAP3K14、NFKB2存在相关性（P均<0.05）。在细胞表型试验中，通过qRT-PCR检测鼻咽癌细胞系中ZNF488 siRNA（mRNA）表达量，从中选择两株表达水平相对较高的SUNE-1和CNE-1。随后评估转染效率，在转染siRNA的实验组，ZNF488表达量明显低于对照组。降低ZNF488表达水平后接受0Gy、2Gy、4Gy、6Gy、8Gy剂量梯度的电离辐射，克隆形成和CCK-8检测结果显示，与对照组相比，实验组的细胞增殖和存活分数显著下降（P均<0.05）。［结论］ 沉默ZNF488后鼻咽癌细胞的增殖能力和存活分数下降，放射敏感性增加，ZNF488可能与放疗敏感性相关。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effect of ZNF488 on radiosensitivity of nasopharyngeal carcinoma(NPC) cells. ［Methods］ Bioinformatics data of ZNF488 in head and neck squamous cell carcinoma were retrieved from GEPIA and UALCAN data platforms. Human NPC SUNE-1 and CNE-1 cells were transfected with ZNF488 siRNA. The transfection efficiency was determined by qRT-PCR and Western blot. The effects of radiation on proliferation and survival of nasopharyngeal carcinoma cells were evaluated by clone formation and CCK8 methods. ［Results］ Based on the bioinformation data platform，the expression level of ZNF488 was correlated with prognosis in head and neck squamous cell carcinoma，and was significantly correlated with expression of MAP3K14 and NFKB2(all P<0.05). In the siRNA transfected group，the expression level of ZNF488 was significantly lower than that of the control group. Transfected NPC cells were received radiation with a dose gradient of 0Gy，2Gy，4Gy，6Gy and 8Gy. The results of clone formation and CCK8 methods showed that cell proliferation and survival scores of the experimental group were significantly decreased compared with the control group(all P<0.05). ［Conclusion］ The proliferation and survival scores of nasopharyngeal carcinoma cells are decreased and the radiosensitivity is increased after ZNF488 silention，indicating that ZNF488 may be associated with radiosensitivity of NPC cells. |
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