| 李志勇,马 鹏,冯 俊.LncRNA-KAT7在鼻咽癌中的表达及对化疗敏感性的影响[J].肿瘤学杂志,2020,26(10):863-868. |
| LncRNA-KAT7在鼻咽癌中的表达及对化疗敏感性的影响 |
| Expression of lncRNA-KAT7 in Nasopharyngeal Carcinoma and Its Effect on Chemosensitivity |
| 投稿时间：2020-04-01 |
| DOI：10.11735/j.issn.1671-170X.2020.10.B003 |
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| 中文关键词: 鼻咽肿瘤 lncRNA-KAT7 化疗敏感性 |
| 英文关键词:nasopharyngeal carcinoma lncRNA-KAT7 chemosensitivity |
| 基金项目:四川省卫健委课题 (17PJ407) |
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| 摘要点击次数: 1215 |
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| 中文摘要: |
| 摘 要：［目的］探讨lncRNA-KAT7在晚期鼻咽癌患者中的表达及对顺铂化疗敏感性的影响。［方法］ 选取86例晚期鼻咽癌患者的组织标本作为研究组，选取同时间段就诊鼻咽良性病变患者的组织标本86例作为对照组。比较鼻咽癌组织、癌旁组织和鼻咽良性组织lncRNA-KAT7的相对表达量；研究组患者诱导化疗后评价疗效，根据疗效分为敏感组和抵抗组，比较lncRNA-KAT7表达水平。比较人鼻咽癌细胞株CNE1、HONE1、C666-1、CNE-2、5-8F及鼻咽上皮细胞株NP69中lncRNA-KAT7表达水平。细胞计数试剂盒（CCK）检测转染KAT7-siRNA过表达载体对细胞增殖和凋亡的影响。［结果］ 鼻咽癌组织lncRNA-KAT7相对表达量为1.05±0.31，低于癌旁组织（1.47±0.48）和鼻咽良性组织（2.59±0.73），差异有统计学意义（P＜0.05）。化疗敏感组lncRNA-KAT7相对表达量为1.23±0.34，高于抵抗组（0.78±0.21），差异有统计学意义（P＜0.05）。NP69细胞中lncRNA-KAT7相对表达量为1.01±0.11，高于CNE1（0.16±0.03）、HONE1（0.31±0.07）、C666-1（0.23±0.04）、CNE-2（0.32±0.20）、5-8F（0.39±0.16）细胞，差异均有统计学意义（P均＜0.05）。KAT7-siRNA组lncRNA-KAT7相对表达量为5.36±1.15，高于阴性对照组（1.27±0.12）和空白对照组（1.24±0.13），差异有统计学意义（P＜0.05）。KAT7-siRNA组的IC50为12.54±3.43，低于阴性对照组（18.23±3.25），差异有统计学意义（P＜0.05）。在顺铂1、4、8、16及32μmol/L浓度下KAT7-siRNA组细胞凋亡率明显高于阴性对照组，差异有统计学意义（P<0.05）。［结论］ lncRNA-KAT7在鼻咽癌组织中低表达，上调lncRNA-KAT7可以提高鼻咽癌对顺铂的敏感性。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the expression of lncRNA-KAT7 in patients with nasopharyngeal carcinoma(NPC) and its effect on chemosensitivity. ［Methods］ The relative expression levels of lncRNA-KAT7 was detected with RT-PCR in cancer tissues and adjacent tissues of 86 NPC patients(NPC group)，and 86 samples of benign nasopharyngeal tissues(control group). The efficacy after induction chemotherapy was evaluated，and patients were divided into a sensitive group and a resistance group according to the efficacy. The expression levels of lncRNA-KAT7 in human nasopharyngeal carcinoma cell lines CNE1，HONE1，C666-1，CNE-2，5-8F and nasopharyngeal epithelial cell line NP69 were compared. Cell counting kit(CCK) was used to detect the proliferation and apoptosis after NPC cells were transfected with KAT7-siRNA. ［Results］ The relative expression of lncRNA-KAT7 in nasopharyngeal carcinoma was 1.05±0.31，which was lower than that in adjacent tissues(1.47±0.48) and benign nasopharyngeal tissue(2.59±0.73)(P<0.05). The relative expression level of lncRNA-KAT7 in the sensitive group was(1.23±0.34)，which was higher than that in the resistance group(0.78±0.21)(P<0.05). The relative expression of lncRNA-KAT7 in NP69 cells was(1.01±0.11)，which was higher than that of NPC cell lines CNE1(0.16±0.03)，HONE1(0.31±0.07)，C666-1(0.23±0.04)，CNE-2(0.32±0.20) and 5-8F(0.39±0.16)(all P<0.05). The relative expression level of lncRNA-KAT7 in the KAT7-siRNA group was(5.36±1.15)，which was higher than that in the negative control group(1.27±0.12) and the blank control group(1.24±0.13)(P<0.05). The IC50 of the KAT7-siRNA group was(12.54±3.43)，which was significantly lower than that of the negative control group(18.23±3.25)(P<0.05). The apoptosis rate of the KAT7-siRNA group was significantly higher than that of the negative control group at the concentrations of cisplatin 1，4，8，16，and 32 μmol/L(P<0.05). ［Conclusion］ lncRNA-KAT7 is under-expressed in nasopharyngeal carcinoma，and up-regulation of lncRNA-KAT7 can increase the sensitivity of nasopharyngeal carcinoma to cisplatin. |
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