| 刘 靓,蔡晓辉,王 婷.多发性骨髓瘤患者骨髓组织中微小RNA-17-5p和PTEN表达及意义[J].肿瘤学杂志,2020,26(9):817-821. |
| 多发性骨髓瘤患者骨髓组织中微小RNA-17-5p和PTEN表达及意义 |
| Expression and Clinical Significance of MicroRNA-17-5p and PTEN in Bone Marrow of Patients with Multiple Myeloma |
| 投稿时间：2020-03-18 |
| DOI：10.11735/j.issn.1671-170X.2020.09.B013 |
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| 中文关键词: 多发骨髓瘤 miR-17-5p PTEN |
| 英文关键词:multiple myeloma miR-17-5p PTEN |
| 基金项目:江苏省科技项目（BK20160283） |
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| 中文摘要: |
| 摘 要：［目的］ 研究多发骨髓瘤（multiple myeloma，MM）患者骨髓液中微小RNA（miRNA，miR）-17-5p及磷酸酶和张力蛋白同源物（phosphatase and tensin homolog，PTEN）的表达及其临床意义。［方法］ 采用荧光实时定量PCR检测82例MM患者及40例健康人群骨髓组织中miR-17-5p及PTEN的表达，统计学分析两组间表达差异以及两者与临床病理特征之间关系。［结果］ 与对照组相比，MM组患者骨髓组织中miR-17-5p表达量明显较高，而PTEN表达量明显较低（t=15.365、11.647，P均<0.001）。MM组患者骨髓组织中miR-17-5p及PTEN表达与D-S分期、髓外浸润有关（t=9.713、3.147，8.901、2.259，P=0.000、0.002，0.000、0.027）。MM组患者骨髓组织中miR-17-5p与PTEN表达呈负相关（r=-0.611，P<0.001）。化疗前、后MM患者骨髓中miR-17-5p、PTEN的相对表达量无显著性差异（t=1.590、1.439，P=0.114、0.152）。化疗有效患者化疗后miR-17-5p表达较化疗前明显较低（t=2.149，P=0.034），而PTEN表达明显较高（t=2.252，P=0.026）。［结论］ MM患者骨髓组织中miR-17-5p表达升高，而PTEN表达降低，两者与D-S分期、髓外浸润有关，有望成为MM诊断治疗的标志物。 |
| 英文摘要: |
| Abstract：［Objective］ To study the expression of microRNA-17-5p(miR-17-5p) and phosphatase and tensin homolog（PTEN）in bone marrow of patients with multiple myeloma(MM) and their clinical significance. ［Methods］ The expressions of miR-17-5p and PTEN in bone marrow of 82 MM patients and 40 healthy subjects were detected by fluorescent real-time quantitative RT-PCR. The correlation of miR-17-5p and PTEN expression with clinicopathological features of MM patients was analyzed. ［Results］ Compared with the control group，the expression of miR-17-5p in bone marrow tissue of patients with MM was significantly higher，while the expression of PTEN was significantly lower（t=15.365 and 11.647，P<0.01）. The expressions of miR-17-5p and PTEN in bone marrow tissue of patients with MM were correlated with DS staging and extramedullary infiltration（t=9.713，3.147 and 8.901，2.259，P=0.000，0.002 and 0.000，0.027）. There was a significant negative correlation between miR-17-5p and PTEN expression in the bone marrow tissue of patients with MM(r=-0.611，P<0.001). There was no significant difference in the relative expression of miR-17-5p and PTEN in the bone marrow of patients with MM before and after chemotherapy(t=1.590，1.439，P=0.114，0.152). After chemotherapy，miR-17-5p expression was significantly lower(t=2.149，P=0.034)，while PTEN expression was significantly higher(t=2.252，P=0.026) than those before chemotherapy in response patients. ［Conclusion］ The expression of miR-17-5p in bone marrow tissue of MM patients is increased，while the expression of PTEN is decreased. Both of them are correlated with the D-S stage of MM and extramedullary infiltration，which indicates that miR-17-5p and PTEN may be used as markers for diagnosis and treatment of multiple myeloma. |
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