| 伊丽娜,寇新红,张晨光.MiR-124靶向调控PKM2基因表达抑制乳腺癌细胞的增殖和转移[J].肿瘤学杂志,2020,26(9):757-761. |
| MiR-124靶向调控PKM2基因表达抑制乳腺癌细胞的增殖和转移 |
| MicroRNA-124 Inhibits Proliferation and Metastasis of Breast Cancer Cells by Targeting PKM2 Gene Expression |
| 投稿时间：2019-12-13 |
| DOI：10.11735/j.issn.1671-170X.2020.09.B001 |
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| 中文关键词: miR-124 PKM2 乳腺癌 增殖 转移 |
| 英文关键词:miR-124 PKM2 breast cancer proliferation metastasis |
| 基金项目:新疆维吾尔自治区自然科学基金（2017D01C379） |
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| 中文摘要: |
| 摘 要：［目的］ 探讨miR-124对乳腺癌MDA-MB231细胞增殖和转移的影响。［方法］ 运用实时荧光定量PCR法、免疫印迹实验检测25例乳腺癌乳腺癌组织及对应癌旁组织中的miR-124、丙酮酸激酶同工酶（PKM2）相对表达量。将pre-miR-124、抗miR-124转染至乳腺癌MDA-MB231细胞中，同时设置阴性对照组，检测三组细胞中miR-124、PKM2基因及蛋白表达量。运用CCK-8法及克隆形成实验检测转染后的MDA-MB231细胞增殖和转移。［结果］ 乳腺癌组织中miR-124（0.92±0.24）的相对表达量显著性少于癌旁组织（1.55±0.28），PKM2的相对表达量更高（0.69±0.18）。pre-miR-124转染MDA-MB231细胞呈现miR-124过表达（0.37±0.08），且其可以反向调控PKM2基因（0.52±0.11）及蛋白表达量（0.19±0.04），抑制细胞的增殖与转移能力。抗miR-124转染MDA-MB231细胞呈现miR-124抑制表达（0.06±0.03），其PKM2基因（2.09±0.08）及蛋白表达量（0.47±0.10）显著性上升，促进细胞增殖与转移（P＜0.05）. ［结论］ miR-124可以通过下调PKM2基因的表达，从而抑制乳腺癌MDA-MB231细胞的增殖和转移。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effect of microRNA-124(miR-124) on proliferation and metastasis of breast cancer cells. ［Methods］ Real-time fluorescence quantitative RT-PCR and immunoblotting were used to detect the relative expression of miR124 and PKM2 mRNA in breast cancer tissues and adjacent tissues of 25 patients with breast cancer. Pre-miR-124 and anti-miR-124 were transfected into breast cancer MDA-MB231 cells，and blank control group was set up to detect the expression of miR-124 and PKM2 mRNA and protein in the three groups. The proliferation and metastasis of transfected MDA-MB231 cells were detected by CCK-8 assay and clonogenic assay. ［Results］ The relative expression of miR-124(0.92±0.24) in breast cancer was significantly lower than that in adjacent breast tissues(1.55±0.28)，and the relative expression of PKM2 mRNA was higher(0.69±0.18). The over-expression of miR-124 in MDA-MB231 cells transfected with pre-miR-124 was 0.37±0.08，and it down regulated expression of PKM2 mRNA(0.52±0.11) and protein(0.19±0.04)，and inhibited cell proliferation and metastasis. AntimiR-124 transfected MDA-MB231 cells showed that the expression of miR-124a was inhibited(0.06±0.03)，while the expression of PKM2 mRNA(2.09±0.08) and protein expression(0.47±0.10) was increased significantly，and the cell proliferation and metastasis were promoted(all P<0.05). ［Conclusion］ MicroRNA-124 can inhibit the proliferation and metastasis of breast cancer MDA-MB231 cells by down-regulating the expression of PKM2 gene. |
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