| 王海川,蔡晓燕,吕 强.SPRED1在结肠癌中的表达及意义[J].肿瘤学杂志,2020,26(8):700-705. |
| SPRED1在结肠癌中的表达及意义 |
| Expression of SPRED1 in Colon Cancer and Its Significance |
| 投稿时间：2019-09-17 |
| DOI：10.11735/j.issn.1671-170X.2020.08.B008 |
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| 中文关键词: SPRED1 结肠肿瘤 增殖 迁移 凋亡 |
| 英文关键词:SPRED1 colon cancer proliferation migration apoptosis |
| 基金项目:上海市浦东新区卫生系统特色专病建设资助——肠癌合并急性肠梗阻（PWZzb2017-19） |
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| 中文摘要: |
| 摘 要：［目的］ 探讨SPRED1在结肠癌中的表达，及其与结肠癌患者预后的相关性，以及对结肠癌细胞增殖、迁移和凋亡的影响。［方法］ 采用实时荧光定量聚合酶链反应（qRT-PCR）检测SPRED1 mRNA在结肠癌细胞和组织标本中的表达，免疫组织化学技术检测SPRED1蛋白在结肠癌组织中的表达。使用小干扰RNA（siRNA）干扰结肠癌细胞内SPRED1表达后，CCK-8试剂盒检测结肠癌细胞增殖能力变化，Transwell小室检测结肠癌细胞迁移能力变化，Annexin V-FITC/PI凋亡试剂盒检测细胞凋亡水平。［结果］ SPRED1 mRNA在结肠癌细胞株和结肠癌组织中表达水平均显著上调（P均<0.001），高表达SPRED1患者的总生存期短于低表达SPRED1患者（HR=2.326，95%CI：1.340～4.062，P<0.05）。干扰SPRED1后，干扰组结肠癌SW480和SW620细胞在36h和48h后增殖能力显著下降（P<0.05），侵袭能力显著下降（SW480：t=42.05，P<0.0001；SW620：t=12.91，P=0.0002），且凋亡水平显著上升（SW480：t=18.46，P<0.05；SW620：t=18.52，P<0.0001）。［结论］SPRED1在结肠癌中表达上调，促进结肠癌的增殖和侵袭。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the expression of SPRED1 in colon cancer and its significance. ［Methods］ The expression of SPRED1 mRNA and protein in cancer tissue and adjacent tissue samples of 45 patients with colon cancer was detected with qRT-PCR and immunohistochemistry，respectively. The expression of SPRED1 mRNA and protein was detected in colon cancer SW480，SW620 cells and normal colon epithelial NCM460 cells with Real-time quantitative RT-PCR(qRT-PCR) and Western blot，respectively. The colon cancer SW480 and SW620 cells were transfected with small interference RNA(si-SPRED1). After down-regulation of SPRED1，cell proliferation，cell migration and cell apoptosis were determined by CCK8 assay，Transwell method and Annexin V-FITC/PI assay，respectively.［Results］ The expression of SPRED1 mRNA and protein was higher in colon cancer tissues than that in adjacent tissues，in cancer cell lines than in normal colon epithelia cells(all P<0.001).The overall survival time of patients with high expression of SPRED1 was shorter than that of patients with low expression of SPRED1(HR=2.326，95%CI：1.340～4.062，P<0.05). After down-regulation of SPRED1，cell proliferation was significantly suppressed(P<0.05). Both SW480 and SW620 cells presented decreased cell migration after down-regulation of SPRED1(SW480：t=42.05，P<0.0001；SW620：t=12.91，P=0.0002). colon cancer Cells transfected with si-SPRED1 showed higher apoptosis level(SW480：t=18.46，P<0.05；SW620：t=18.52，P<0.0001). ［Conclusion］ SPRED1 is upregulated in colon cancer，which is associated with the increased cell proliferation，cell invasion and decreased cell apoptosis. |
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