| 吴 飞,郑兰东,刘 利.miR-629对胰腺癌细胞增殖和迁移的影响[J].肿瘤学杂志,2020,26(8):695-699. |
| miR-629对胰腺癌细胞增殖和迁移的影响 |
| Effect of MiR-629 on Proliferation and Migration of Pancreatic Cancer Cells |
| 投稿时间：2019-09-17 |
| DOI：10.11735/j.issn.1671-170X.2020.08.B007 |
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| 中文关键词: 胰腺肿瘤 miR-629 增殖 迁移 SIRT3 |
| 英文关键词:pancreatic cancer miR-629 proliferation migration SIRT3 |
| 基金项目:河南省科技厅科技攻关项目(192102310364) |
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| 中文摘要: |
| 摘 要：［目的］ 探讨miR-629在胰腺癌组织中的表达及其对胰腺癌细胞增殖和迁移的影响。［方法］ 选取手术切除的胰腺癌组织及其癌旁组织各72例，采用RT-PCR检测miR-629的mRNA表达水平。构建NC-shRNA和miR-629-shRNA慢病毒稳定细胞系，采用CCK-8法检测miR-629对细胞增殖的影响；采用Transwell检测miR-629对细胞迁移的影响；采用双荧光素酶报告基因和Western blot验证miR-629与SIRT3的靶向关系。［结果］ 与癌旁组织（0.40±0.04）比较，胰腺癌组织中miR-629的mRNA相对表达水平（1.71±0.15）显著增加（t=3.901，P<0.001）；与NC-shRNA细胞比较，miR-629-shRNA细胞的增殖能力均显著下降（F=2.719，P<0.001）；与NC-shRNA细胞（140.22±19.37）比较，miR-629-shRNA细胞的迁移能力（63.91±7.44）显著下降（t=4.612，P<0.001）；荧光素酶报告基因显示，转染SIRT3-WT后，miR-629-shRNA细胞的相对荧光素酶活性（0.33±0.05）较NC-shRNA细胞（1.25±0.12）显著增加（t=4.109，P<0.001）；western blot结果表明miR-629-shRNA细胞中SIRT3的蛋白表达水平（0.44±0.03）较NC-shRNA细胞（1.31±0.11）明显增加（t=3.692，P<0.001）。［结论］ miR-629在胰腺癌组织中高表达，可能通过靶向下调SIRT3的表达促进胰腺癌细胞的增殖和迁移，有望为胰腺癌的临床诊疗提供新的思路。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the expression of miR-629 in pancreatic cancer and its effect on proliferation and migration of pancreatic cancer cells. ［Methods］ Seventy-two samples of pancreatic cancer tissue and corresponding adjacent tissue were collected，and the expression of miR-629 was detected by RT-PCR. The NC-shRNA and miR-629-shRNA lent viral stable human pancreatic cancer PANC1cell lines were constructed. The effect of miR-629 on cell proliferation was detected by CCK-8 method；the effect of miR-629 on cell migration was analyzed byTranswell. The targeting relationship between miR-629 and SIRT3was verified by the dual luciferase reporter gene and Western blot.［Results］ Compared with adjacent tissues，the relative mRNA expression of miR-629 in pancreatic cancer tissues was significantly increased(0.40±0.04 vs 1.71±0.15，t=3.901，P<0.001). Compared with NC-shRNA cells，the proliferation activity of miR-629-shRNA cells were significantly decreased(F=2.719，P<0.001). Compared with NC-shRNA transfected PANC1 cells，the migration ability of miR-629-shRNA transfected PANC1 cellssignificantly decreased(140.22±19.37 vs 63.91±7.447，t=4.612，P<0.001). Luciferase reporter gene showed that the relative luciferase activity of miR-629-shRNA cells significantly increased compared with NC-shRNA cellsafter transfection of SIRT3-WT(1.25±0.12 vs 0.33±0.05，t=4.109，P<0.001). Western blot showed that the expression of SIRT3 in miR-629-shRNA cellssignificantly higher than that in NC-shRNA cells(1.31±0.11 vs 0.44±0.03，t=3.692，P<0.001).［Conclusion］ The high expression of miR-629 in pancreatic cancer tissues may promote the proliferation and migration of pancreatic cancer cells by down-regulation of SIRT3 expression，which may provide new ideas for the clinical diagnosis and treatment of pancreatic cancer. |
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