| 陈苏娟,李瑞中.miR-218-5p靶向N-cadherin调节胃癌细胞迁移、侵袭分析[J].肿瘤学杂志,2020,26(8):684-688. |
| miR-218-5p靶向N-cadherin调节胃癌细胞迁移、侵袭分析 |
| MiR-218-5p Regulates Migration and Invasion of Gastric Cancer Cells via Targeting N-Cadherin |
| 投稿时间：2020-05-11 |
| DOI：10.11735/j.issn.1671-170X.2020.08.B005 |
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| 中文关键词: 胃肿瘤 miR-218-5p N-cadherin 迁移 侵袭 |
| 英文关键词:gastric cancer miR-218-5p N-cadherin migration invasion |
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| 中文摘要: |
| 摘 要：［目的］ 研究miR-218-5p靶向N-钙黏蛋白（N-cadherin）调节胃癌细胞迁移、侵袭的作用及机制。［方法］ 收集28例胃癌组织及癌旁组织，检测miR-218-5p及N-cadherin的表达水平；培养MNK45胃癌细胞，分为转染阴性对照（NC）mimic的NC组和转染miR-218-5p mimic的miR-218-5p组，检测细胞的迁移及侵袭数目、N-cadherin的mRNA及蛋白表达水平、N-cadherin双荧光素酶报告基因的荧光活力。［结果］胃癌组织中miR-218-5p的表达水平（0.45±0.09）显著低于癌旁组织的（0.71±0.22）（P<0.05），N-cadherin的表达水平（0.93±0.20）显著高于癌旁组织的（0.67±0.14）（P<0.05），且miR-218-5p的表达水平与N-cadherin的表达水平呈负相关（r=-0.201，P<0.05）。miR-218-5p组MNK45胃癌细胞的迁移数目（65.57±11.49）及侵袭数目（67.11±10.34）均少于NC组（113.84±20.13，98.72±18.57；P均<0.05），N-cadherin的mRNA及蛋白表达水平、野生型N-cadherin双荧光素酶报告基因的荧光活力低于NC组（P<0.05）。［结论］ miR-218-5p在胃癌中低表达，过表达miR-218-5p能够抑制胃癌细胞的迁移和侵袭，靶向抑制N-cadherin是可能的分子机制。 |
| 英文摘要: |
| Abstract：［Objective］ To study the effect of miR-218-5p on regulating the migration and invasion of gastric cancer cells and its mechanism. ［Methods］ The cancer and adjacent tissue samples were collected from 28 gastric cancer patients. The expression levels of miR-218-5p and N-cadherin were measured. Gastric cancer MNK45 cells were transfected with miRNA mimic(NC group) or miR-218-5p(miR-218-5p group). The cell migration and invasion，expression levels of N-cadherin mRNA and protein，fluorescence activity of N-cadherin double luciferase reporter gene were measured. ［Results］ The expression level of miR-218-5p in gastric cancer was significantly lower than of paracancerous tissue(0.45±0.09 vs 0.71±0.22，P<0.05)，and the expression level of N-cadherin was significantly higher than of paracancerous tissue(0.93±0.20 vs 0.67±0.14，P<0.05)；the expression level of miR-218-5p was negatively correlated with the expression level of N-cadherin(r=-0.201，P<0.05). The migrating(65.57±11.49) and invading(67.11±10.34) MNK45 cells in miR-218-5p group were less than those in NC group(113.84±20.13，98.72±18.57，P<0.05) .The mRNA and protein expression level of N-cadherin，and the fluorescence activity of wild-type N-cadherin double fluorescence reporter gene in miR-218-5p group were lower than those of NC group(P<0.05). ［Conclusion］ The expression level of miR-218-5p is low in gastric cancer. Overexpression of miR-218-5p can inhibit the migration and invasion of gastric cancer cells，which is associated with the inhibition of N-cadherin. |
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