陈苏娟,李瑞中.miR-218-5p靶向N-cadherin调节胃癌细胞迁移、侵袭分析[J].肿瘤学杂志,2020,26(8):684-688.
miR-218-5p靶向N-cadherin调节胃癌细胞迁移、侵袭分析
MiR-218-5p Regulates Migration and Invasion of Gastric Cancer Cells via Targeting N-Cadherin
投稿时间:2020-05-11  
DOI:10.11735/j.issn.1671-170X.2020.08.B005
中文关键词:  胃肿瘤  miR-218-5p  N-cadherin  迁移  侵袭
英文关键词:gastric cancer  miR-218-5p  N-cadherin  migration  invasion
基金项目:
作者单位
陈苏娟 盐城市第一人民医院肿瘤治疗中心 
李瑞中 盐城市第一人民医院肿瘤治疗中心 
摘要点击次数: 1811
全文下载次数: 337
中文摘要:
      摘 要:[目的] 研究miR-218-5p靶向N-钙黏蛋白(N-cadherin)调节胃癌细胞迁移、侵袭的作用及机制。[方法] 收集28例胃癌组织及癌旁组织,检测miR-218-5p及N-cadherin的表达水平;培养MNK45胃癌细胞,分为转染阴性对照(NC)mimic的NC组和转染miR-218-5p mimic的miR-218-5p组,检测细胞的迁移及侵袭数目、N-cadherin的mRNA及蛋白表达水平、N-cadherin双荧光素酶报告基因的荧光活力。[结果]胃癌组织中miR-218-5p的表达水平(0.45±0.09)显著低于癌旁组织的(0.71±0.22)(P<0.05),N-cadherin的表达水平(0.93±0.20)显著高于癌旁组织的(0.67±0.14)(P<0.05),且miR-218-5p的表达水平与N-cadherin的表达水平呈负相关(r=-0.201,P<0.05)。miR-218-5p组MNK45胃癌细胞的迁移数目(65.57±11.49)及侵袭数目(67.11±10.34)均少于NC组(113.84±20.13,98.72±18.57;P均<0.05),N-cadherin的mRNA及蛋白表达水平、野生型N-cadherin双荧光素酶报告基因的荧光活力低于NC组(P<0.05)。[结论] miR-218-5p在胃癌中低表达,过表达miR-218-5p能够抑制胃癌细胞的迁移和侵袭,靶向抑制N-cadherin是可能的分子机制。
英文摘要:
      Abstract:[Objective] To study the effect of miR-218-5p on regulating the migration and invasion of gastric cancer cells and its mechanism. [Methods] The cancer and adjacent tissue samples were collected from 28 gastric cancer patients. The expression levels of miR-218-5p and N-cadherin were measured. Gastric cancer MNK45 cells were transfected with miRNA mimic(NC group) or miR-218-5p(miR-218-5p group). The cell migration and invasion,expression levels of N-cadherin mRNA and protein,fluorescence activity of N-cadherin double luciferase reporter gene were measured. [Results] The expression level of miR-218-5p in gastric cancer was significantly lower than of paracancerous tissue(0.45±0.09 vs 0.71±0.22,P<0.05),and the expression level of N-cadherin was significantly higher than of paracancerous tissue(0.93±0.20 vs 0.67±0.14,P<0.05);the expression level of miR-218-5p was negatively correlated with the expression level of N-cadherin(r=-0.201,P<0.05). The migrating(65.57±11.49) and invading(67.11±10.34) MNK45 cells in miR-218-5p group were less than those in NC group(113.84±20.13,98.72±18.57,P<0.05) .The mRNA and protein expression level of N-cadherin,and the fluorescence activity of wild-type N-cadherin double fluorescence reporter gene in miR-218-5p group were lower than those of NC group(P<0.05). [Conclusion] The expression level of miR-218-5p is low in gastric cancer. Overexpression of miR-218-5p can inhibit the migration and invasion of gastric cancer cells,which is associated with the inhibition of N-cadherin.
在线阅读   查看全文  查看/发表评论  下载PDF阅读器