| 马春兰,张宝亮,张常虹.莪术醇对乳腺癌细胞增殖凋亡及JAK2/STAT3信号通路的影响[J].肿瘤学杂志,2020,26(7):616-620. |
| 莪术醇对乳腺癌细胞增殖凋亡及JAK2/STAT3信号通路的影响 |
| Effect of Curcumol on JAK2/STAT3 Signaling Pathway in Breast Cancer Cells |
| 投稿时间：2019-09-10 |
| DOI：10.11735/j.issn.1671-170X.2020.07.B010 |
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| 中文关键词: 莪术醇 乳腺癌 JAK2/STAT3 细胞凋亡 细胞增殖 |
| 英文关键词:curcumol breast cancer JAK2/STAT3 apoptosis cell proliferation |
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| 中文摘要: |
| 摘 要：［目的］ 探讨莪术醇对乳腺癌细胞增殖凋亡及JAK2/STAT3信号通路的影响。［方法］ 以不同浓度（0、12.5、25、50、100μg/ml）莪术醇作用于乳腺癌MCF-7细胞，MTT法检测MCF-7细胞增殖抑制率，流式细胞术观察细胞周期凋亡分布，Hoechst33258染色法观察细胞凋亡形态学变化，逆转录聚合酶链反应法检测细胞酪氨酸激酶2（JAK2）、 信号转导子和转录激活子3（STAT3）信使核糖核酸相对表达量，蛋白印迹法检测细胞磷酸化JAK2( P-JAK2)、磷酸化STAT3(P-STAT3)蛋白相对表达量。［结果］ 莪术醇对MCF-7细胞增殖具有明显抑制作用，且抑制率随药物作用时间及作用浓度的增加而上升。随着莪术醇作用浓度的增加，细胞凋亡率也有所上升［（4.27%±0.82%）、(4.54%±1.21%)、(19.32%±1.87%)、(29.58%±2.92%)、(33.74%±3.91%)、(42.94%±2.81%)］。细胞周期结果显示，莪术醇可将细胞周期阻滞在G0/G1期，从而抑制细胞增殖。Western blot结果显示，莪术醇可抑制JAK2、STAT3磷酸化的表达，而RT-PCR结果显示，莪术醇作用细胞后，JAK2、STAT3 mRNA表达受到明显抑制。［结论］ 莪术醇作用于乳腺癌MCF-7细胞G0/G1期，从而抑制细胞增殖，诱导细胞凋亡，其机制可能是通过JAK2/STAT3信号通路来实现。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effect of curcumol on JAK2/STAT3 signaling pathway in breast cancer cells. ［Methods］ Breast cancer MCF-7 cells were treated with different concentrations of curcumol(0，12.5，25，50，100 μg/ml). The proliferation inhibition rate of MCF-7 cells was detected by MTT assay. Cell apoptosis was observed by flow cytometry. Hoechst33258 staining method was used to observe the morphological changes of cell apoptosis. The relative expression of JAK2 and STAT3 mRNA was detected by RT-PCR. The relative expression of P-JAK2 and P-STAT3 protein was detected by Western blot. ［Results］ The curcumol had a significant inhibitory effect on the proliferation of MCF-7 cells，and the inhibition rate increased with the time and concentration of the drug. As the concentration of curcumol increased，the apoptosis rate also increased［（4.27%±0.82%），（4.54%±1.21%），（19.32%±1.87%），（29.58%±2.92%），（33.74%±3.91%），（42.94%±2.81%）］. Cytometry showed that curcumol arrested the cell cycle in G0/G1 phase，thereby inhibiting cell proliferation. Western blot showed that curcumol inhibited the expression of JAK2 and STAT3 phosphorylation，and RT-PCR showed that JAK2 and STAT3 mRNA expression was significantly inhibited after cells were treatment with curcumol. ［Conclusion］ Curcumol acts on G0/G1 phase of breast cancer MCF-7 cells，inhibiting cell proliferation and inducing apoptosis，in which JAK2/STAT3 signaling pathway may be involved. |
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