| 申友奎,徐 良,王雁秋.RNASE4异常表达对胶质瘤细胞侵袭迁移的影响及机制研究[J].肿瘤学杂志,2020,26(7):610-615. |
| RNASE4异常表达对胶质瘤细胞侵袭迁移的影响及机制研究 |
| Effect of RNASE4 Abnormal Expression on Invasion and Migration of Glioma Cells and Its Mechanism |
| 投稿时间：2020-02-25 |
| DOI：10.11735/j.issn.1671-170X.2020.07.B009 |
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| 中文关键词: 胶质瘤 RNASE4 侵袭 迁移 |
| 英文关键词:glioma RNASE4 migration invasion |
| 基金项目:浙江省自然科学基金（Q19H160215） |
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| 摘要点击次数: 1170 |
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| 中文摘要: |
| 摘 要：［目的］ 阐明RNASE4在胶质瘤细胞迁移和侵袭中的潜在作用及机制。［方法］ 利用实时定量PCR（qPCR）检测RNASE4在正常胶质细胞和胶质瘤细胞系中的表达水平。将胶质瘤U87细胞分为对照组、siRNA-NC组和siRNA-RNASE4组。在体外细胞中瞬时转染siRNA-RNASE4，利用CCK-8法检测胶质瘤细胞U87增殖率。采用伤口愈合实验和Transwell小室实验检测U87细胞的侵袭和迁移能力。通过定量PCR和Western blot实验检测各组细胞中相关因子的表达。［结果］ RNASE4在胶质瘤细胞系T98（1.34±0.06，P<0.01）、A172（1.79±0.10，P<0.01）、U251（1.86±0.09，P<0.01）、U118（2.19±0.17，P<0.01）和U87（2.64±0.17，P<0.01）中较NHAs细胞系均异常高表达（1.00±0.05）。在胶质瘤细胞U87中下调RNASE4表达引起细胞增殖活力下降（48h，P<0.05；72h，P<0.01）。同时，siRNA-RNASE4组U87细胞的划痕愈合能力（46.0±4.6 vs 87.2±3.6，P<0.01）以及细胞侵袭能力（72.5±4.7 vs 147.8±16.8，P<0.01）均明显降低。下调RNASE4表达抑制了BCL2、MMP9基因表达，并促进各组细胞中E-cadherin表达（P<0.01）。［结论］ RNASE4能够通过调节BCL2、MMP9等因子的表达来调节胶质瘤细胞的增殖与迁移能力，发挥促癌因子的作用。 |
| 英文摘要: |
| Abstract：［Objective］ To elucidate the potential role and mechanism of RNASE4 in glioma cell migration and invasion. ［Methods］ Real-time quantitative PCR(qPCR) was used to detect the expression level of RNASE4 in normal glioma and glioma cell lines. Glioma U87 cells were divided into control group，siRNA-NC group and siRNA-RNASE4 group. The proliferation rate of glioma cells U87 was detected by CCK-8 method after transient transfection of siRNA-RNASE4 in vitro. The invasion and migration of U87 cells were examined by wound healing and Transwell assay. Quantitative PCR and Western blot were used to detect the expression of related factors in each group. ［Results］ RNASE4 was abnormally highly expressed in glioma cell lines T98(1.34±0.06，P<0.01)，A172(1.79±0.10，P<0.01)，U251(1.86±0.09，P<0.01)，U118(2.19±0.17，P<0.01) and U87(2.64±0.17，P<0.01) compared with NHAs cell(1.00±0.05). Down-regulation of RNASE4 expression in glioma cells U87 resulted in decreased cell proliferation activity(48h，P<0.05；72h，P<0.01). At the same time，the scratch healing ability(46.0±4.6 vs 87.2±3.6，P<0.01) and cell penetrating ability(72.5±4.7 vs 147.8±16.8，P<0.01) of the siRNA-RNASE4 group U87 cells were significantly decreased. In addition，down-regulated RNASE4 expression inhibited the expression of BCL2 and MMP9 genes，and promoted the expression of E-cadherin in each group(P<0.01). ［Conclusion］ RNASE4 can regulate the proliferation and migration of glioma cells by regulating the expression of BCL2，MMP9 and other factors，and play the role of pro-cancer factors. |
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