| 周晓华,李 合,田尤京.结肠癌中Claudin-1表达与血清炎症因子的相关性及临床意义[J].肿瘤学杂志,2020,26(6):517-521. |
| 结肠癌中Claudin-1表达与血清炎症因子的相关性及临床意义 |
| Correlation between Claudin-1 and Serum Inflammatory Cytokines in Colon Cancer and Their Clinical Significance |
| 投稿时间：2019-08-22 |
| DOI：10.11735/j.issn.1671-170X.2020.06.B010 |
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| 中文关键词: 结肠肿瘤 Claudin-1 炎症因子 临床病理特征 预后 |
| 英文关键词:colon cancer Claudin-1 inflammatory cytokines clinicopathologic feature prognosis |
| 基金项目:海南省自然科学基金（812201） |
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| 摘要点击次数: 1202 |
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| 中文摘要: |
| 摘 要：［目的］ 探讨结肠肿瘤组织中紧密连接蛋白Claudin-1的表达与血清炎症因子的相关性，以及它们与患者临床病理特征、预后的关系。［方法］ 选择结肠癌患者57例，采用RT-PCR和Western Blot方法检测肿瘤组织和癌旁组织中Claudin-1的表达，采用ELISA的方法检测患者血清炎症因子水平，分析两者的相关性以及两者与患者临床病理特征的关系。绘制3年生存曲线，分析Claudin-1和炎症因子与患者预后的关系。［结果］ Claudin-1 mRNA和蛋白均在癌旁组织中低表达，在肿瘤组织中高表达(1.34±0.36 vs 3.24±1.47，P=0.005；0.92±0.28 vs 2.41±1.25，P=0.007)；肿瘤患者血清TNF-α、IL-6、IL-1β水平均明显高于健康人群（P=0.010、0.003、0.001）；Spearman检验发现Claudin-1表达和血清炎症因子水平呈正相关(P<0.05)；Claudin-1蛋白表达及血清炎症因子水平均与TNM分期、分化程度、淋巴结转移有关（P<0.05）。Log-rank (Mantel-Cox)检验发现Claudin-1表达和血清炎症因子水平均是影响患者预后的因素。 ［结论］ 结肠肿瘤组织中Claudin-1的表达和血清炎症因子存在相关性，两者均是患者预后的影响因素。 |
| 英文摘要: |
| Abstract：［Objective］ To explore the relationship between the expression of claudin-1 in colon tumor tissues and serum inflammatory cytokines，and their relationship with the clinicopathological characteristics and prognosis of patients.［Methods］ The mRNA and protein expression of Claudin-1 in tumor tissues and pericancerous tissues of 57 colon cancer patients were detected by RT-PCR and Western blot，respectively. The serum levels of inflammatory cytokines of patients were measured by ELISA. The relationship between expression of claudin-1 and serum inflammatory cytokines with clinicopathological characteristics and the prognosis was analyzed. ［Results］ The expression of claudin-1 mRNA and protein in cancer tissues was significantly higher than that in pericancerous tissues(3.24±1.47 vs. 1.34±0.36，P=0.005；2.41±1.25 vs. 0.92±0.28，P=0.007). Serum levels of TNF-α，IL-6，IL-1β of patients were significantly higher than those of healthy subjects(P=0.010，0.003 and 0.001). Spearman test revealed a positive correlation between claudin-1 expression and serum inflammatory cytokines(P<0.05). Chi-square analysis found that both the levels of claudin-1 and inflammatory cytokines were related to TNM stage，degree of differentiation and lymph node metastasis(all P<0.05). Log-rank(Mantel-Cox) test showed that both the levels of claudin-1 expression and inflammatory cytokines were associated with the prognosis of patients. ［Conclusion］ The expression of claudin-1 in colon tumor tissues is correlated with serum inflammatory cytokines，and both of them are factors affecting the prognosis of patients. |
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