| 范 羿,周 宇,高 伟.穿心莲内酯通过抑制PI3K/AKT通路对人肝癌HEPG2细胞凋亡的影响[J].肿瘤学杂志,2020,26(6):496-500. |
| 穿心莲内酯通过抑制PI3K/AKT通路对人肝癌HEPG2细胞凋亡的影响 |
| Effect of Andrographolide on Apoptosis of Human Hepatoma HepG2 Cells by Inhibiting PI3K/AKT Pathway |
| 投稿时间：2019-08-14 |
| DOI：10.11735/j.issn.1671-170X.2020.06.B006 |
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| 中文关键词: 穿心莲内酯 HEPG2细胞 PI3K/AKT通路 细胞凋亡 细胞增殖 |
| 英文关键词:andrographolide HEPG2 cells PI3K/AKT pathway apoptosis cell proliferation |
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| 中文摘要: |
| 摘 要：［目的］ 探讨穿心莲内酯通过抑制磷脂酰肌醇3-激酶（PI3K）/蛋白激酶 B（AKT）通路对人肝癌细胞凋亡的影响。［方法］ 将人肝癌细胞株HEPG2分为对照组、低浓度穿心莲内酯组、中浓度穿心莲内酯组、高浓度穿心莲内酯组，低、中、高浓度穿心莲内酯组分别加入培养基稀释的终浓度为10、30、50 μmol/L的穿心莲内酯，对照组加入等剂量的培养基。采用四甲基偶氮唑盐(MTT)法检测各组HEPG2细胞增殖率，流式细胞术检测各组HEPG2细胞凋亡率，Western blot法检测各组HEPG2细胞PI3K、p-AKT蛋白表达水平。［结果］低浓度穿心莲内酯组HEPG2细胞增殖率及PI3K、p-AKT蛋白表达水平较对照组明显下降（Q=2.942、9.836、6.348，P＜0.05）；中浓度穿心莲内酯组HEPG2细胞增殖率及PI3K、p-AKT蛋白表达水平较低浓度穿心莲内酯组明显下降（Q=2.942、13.832、10.691，P＜0.05）；高浓度穿心莲内酯组HEPG2细胞增殖率及PI3K、p-AKT蛋白表达水平较中浓度穿心莲内酯组明显下降（Q=13.411、19.058、16.371，P＜0.05）。低浓度穿心莲内酯组HEPG2细胞凋亡率较对照组明显升高（Q=83.662，P<0.05）；中浓度穿心莲内酯组HEPG2细胞凋亡率较低浓度穿心莲内酯组明显升高（Q=147.267，P<0.05）；高浓度穿心莲内酯组HEPG2细胞凋亡率较中浓度穿心莲内酯组明显升高（Q=241.925，P<0.05）。［结论］ 穿心莲内酯可能通过抑制PI3K/AKT通路抑制人肝癌细胞增殖，促进人肝癌细胞凋亡，可为临床治疗肝癌提供新方向。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effect of andrographolide on apoptosis of human hepatoma HepG2 cells and its relation with phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT) pathway. ［Methods］ Human hepatoma HepG2 cells were divided into control group，low andrographolide concentration group(10 μmol/L)，medium andrographolide concentration group(30 μmol/L) and high andrographolide concentration group(50 μmol/L). The proliferation rate of HEPG2 cells was detected by MTT，the apoptosis rate of HEPG2 cells was detected by flow cytometry，and the expression levels of PI3K and p-AKT protein in HEPG2 cells were detected by Western blot. ［Results］ The proliferation rate and the expression of PI3K and p-AKT protein in the control group were higher than those in low，medium and high andrographolide groups(Q=2.942，9.836，6.348，P<0.05；Q=2.942，13.832，10.691，P<0.05 and Q=13.411，19.058，16.371，P<0.05). The apoptosis rate of HEPG2 cells in control group was lower than that in low，medium and high andrographolide groups(Q=83.662，P<0.05，Q=147.267，P<0.05 and Q=241.925，P<0.05). ［Conclusion］ Andrographolide may inhibit the proliferation and promote the apoptosis of human hepatocellular carcinoma HepG2 cells by inhibiting PI3K/AKT pathway，and also promote the apoptosis of HepG2 cells，which may provide a new direction for clinical treatment of hepatocellular carcinoma. |
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