| 孙 盈,杨 锋,夏靖华.HIP1影响食管鳞癌Akt/GSK3β信号通路及EMT相关蛋白表达分析[J].肿瘤学杂志,2020,26(5):407-412. |
| HIP1影响食管鳞癌Akt/GSK3β信号通路及EMT相关蛋白表达分析 |
| Effect of HIP1 on Expression of EMT-related Proteins in Esophageal Squamous Cell Carcinoma Through Akt/GSK3β Signaling Pathway |
| 投稿时间：2019-09-17 |
| DOI：10.11735/j.issn.1671-170X.2020.05.B008 |
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| 中文关键词: 亨廷顿蛋白相互作用蛋白1 上皮间充质转化 食管鳞癌 |
| 英文关键词:huntingtin interacting protein 1 epithelial mesenchymal transformation esophageal squamous cell carcinomas |
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| 中文摘要: |
| 摘 要：［目的］ 探讨亨廷顿蛋白相互作用蛋白1（huntingtin interacting protein 1，HIP1）促进食管鳞癌转移的作用及机制。［方法］采用qRT-PCR及Western blot检测Akt抑制剂处理HIP1高或低表达食管鳞癌细胞系中HIP1、GSK3β及EMT标志性分子E-cadherin和Vimentin的表达。采用IHC检测食管鳞癌组织样本中目的分子的表达，并分析其表达相关性。［结果］ qRT-PCR和Western blot结果显示：与对照组、shRNA-对照组相比，shRNA-HIP1组细胞中Akt、GSK3β及Vimentin基因和蛋白表达显著降低（P<0.001），E-cadherin基因和蛋白表达显著升高（P<0.001）；与对照组、OE-对照组相比，OE-HIP1组细胞中Akt、GSK3β及Vimentin基因和蛋白表达显著升高（P<0.001），E-cadherin基因和蛋白表达显著降低（P<0.001）。IHC及相关性分析结果显示，HIP1与GSK3β、Vimentin表达呈正相关（r=0.336，P<0.001；r=0.561，P<0.001），与E-Cadherin表达呈负相关（r=-0.169，P=0.027）；GSK3β与Vimentin表达呈正相关（r=0.317，P<0.001），与E-Cadherin表达呈负相关（r=-0.171，P=0.025）。Akt抑制剂处理HIP1高或低表达食管鳞癌细胞系结果显示，Akt抑制剂处理后E-cadherin表达升高，GSK3β及Vimentin表达降低。［结论］HIP1 可能通过激活Akt/GSK3β信号通路促进食管鳞癌EMT的发生。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effect of huntingtin interacting protein 1(HIP1) on epithelial-mesenchymal transition(EMT) related proteins in esophageal squamous cell carcinomas(ESCC) and its mechanism. ［Methods］ The protein expressions of HIP1，GSK3β，EMT related molecules E-cadherin and Vimentin in cancer tissue samples from 173 ESCC patients were detected by immunohistochemistry(IHC). The mRNA and protein expressions of above indicators were also detected by qRT-PCR and Western blot in ESCC cell lines EC109 and Kyse30，respectively. ［Results］ IHC and correlation analysis showed that HIP1 expression was positively correlated with GSK3βand Vimentin(r=0.336，P<0.001；r=0.561，P<0.001)，and was negatively correlated with E-cadherin expression(r=-0.169，P=0.027).GSK3βexpression was positively correlated with Vimentin expression(r=0.317，P<0.001) and negatively correlated with E-cadherin expression(r=-0.171，P=0.025). The qRT-PCR and Western blot showed that，compared with the control group and the shRNA-control group，the mRNA and protein expressions of Akt，GSK3β and Vimentin in the shRNA-HIP1 group were significantly decreased(P<0.001)，while the expression of E-cadherin was significantly increased(P<0.001).Compared with control group and OE-control group，the mRNA and protein expressions of Akt，GSK3β，and Vimentin in OE-HIP1 group were significantly increased(P<0.001)，while the expression of E-cadherin were significantly decreased(P<0.001). The expression of E-cadherin was increased，while the expression of GSK3β and Vimentin was decreased in EC109 and Kyse30 cells treated with Akt inhibitor. ［Conclusion］ HIP1 may promote EMT through activation of Akt/GSK3β signaling pathway in ESCC. |
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