| 李婷秀,冯玉瑰,刘从香.miR-802在原发性肝细胞癌中的表达及意义[J].肿瘤学杂志,2020,26(4):313-317. |
| miR-802在原发性肝细胞癌中的表达及意义 |
| Expression of miR-802 in Primary Hepatocellular Carcinoma and Its Clinical Significances |
| 投稿时间：2019-06-25 |
| DOI：10.11735/j.issn.1671-170X.2020.04.B008 |
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| 中文关键词: 肝细胞肿瘤 miR-802 细胞 预后 |
| 英文关键词:hepatocellular carcinoma miR-802 cell prognosis |
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| 中文摘要: |
| 摘 要：［目的］ 检测微小RNA（miR）-802在原发性肝细胞癌（hepatocellular carcinoma，HCC）组织与细胞中的表达水平，分析miR-802在HCC中的临床意义。［方法］ 采用qRT-PCR检测HCC癌组织与癌旁组织中miR-802的表达水平，及Hep3B、HCCLM3与HL-7702细胞的表达水平。分析miR-802表达水平与HCC临床病理特征的关系。采用Kaplan-Meier方法计算生存曲线，以Log-rank检验评估miR-802表达水平与HCC预后的关系。通过Cox比例风险回归模型单因素和多因素分析评估HCC的预后指标。［结果］ HCC癌组织中miR-802的表达水平显著低于癌旁组织（P<0.05），且Hep3B、HCCLM3细胞中miR-802的表达水平亦显著低于HL-7702细胞（P<0.05）。Ⅲ+Ⅳ期癌组织中miR-802的表达水平低于Ⅰ+Ⅱ期癌组织（P<0.05），血管转移患者癌组织中miR-802的表达水平低于非血管转移患者癌组织（P<0.05）。miR-802低表达组患者的5年生存率显著低于高表达组(P<0.05）。单因素分析显示，低表达miR-802、高TNM分期及阳性血管转移是HCC患者预后不良的预测指标（P<0.05）。多因素分析显示，低表达miR-802是HCC患者预后不良的唯一独立预测指标（P<0.05）。［结论］ HCC组织和细胞中miR-802表达下调，低表达miR-802是HCC患者预后不良的独立预测指标。 |
| 英文摘要: |
| Abstract：［Objective］ To detect the expression levels of miR-802 in primary hepatocellular carcinoma(HCC)，and to analyze its clinical significances. ［Methods］ The expression levels of miR-802 in 52 samples of HCC cancer tissues and corresponding adjacent tissues，as well as in human HCC Hep3B，HCCLM3 cells and human normal liver HL-7702 cells were detected by qRT-PCR. The relationship between miR-802 expression levels and clinicopathological features of HCC was analyzed. Survival curves were calculated by Kaplan-Meier method and the relationship between miR-802 expression levels and HCC prognosis was assessed by log-rank test. The prognostic indicators of HCC were assessed by Cox proportional hazards model for univariate and multivariate Logistic regression analysis. ［Results］ The expression levels of miR-802 in HCC tumor tissues was significantly lower than that in adjacent tissues(P<0.05). The expression levels of miR-802 in Hep3B and HCCLM3 cells were also significantly lower than that in HL-7702 cells(P<0.05). The expression levels of miR-802 in tumor tissues of Ⅲ+Ⅳ stages were lower than those in tumor tissues of Ⅰ+Ⅱ stages(P<0.05)，and its expression levels in tumor tissues of patients with positive vascular metastasis was also lower than those in tumor tissues of patients with non-vascular metastasis(P<0.05). The 5-years overall survival rate of patients with low expression of miR-802 was significantly lower than that of patients with high expression of miR-802(P<0.05). Univariate analysis showed that low expression of miR-802，high TNM stage and positive vascular metastasis were prognostic indicators of poor prognosis in patients with HCC(P<0.05). Multivariate analysis showed that low expression of miR-802 was the only independent predictor of poor prognosis in HCC patients(P<0.05). ［Conclusion］ The expression of miR-802 is down-regulated in HCC tissues and cells. Low expression of miR-802 is an independent prognostic indicator of poor prognosis of HCC patients. |
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