| 宋炬东,丁云贞,张 超.Pannexin1通道对乳腺癌细胞MCF-7细胞增殖和侵袭迁移的影响及可能机制[J].肿瘤学杂志,2020,26(3):210-215. |
| Pannexin1通道对乳腺癌细胞MCF-7细胞增殖和侵袭迁移的影响及可能机制 |
| Effect of Pannexin1 Channel on Proliferation and Migration in Breast Cancer Cell MCF-7 and Its Mechanism |
| 投稿时间：2019-08-29 |
| DOI：10.11735/j.issn.1671-170X.2020.03.B009 |
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| 中文关键词: 乳腺癌 Pannexin1 增殖 侵袭 迁移 |
| 英文关键词:breast cancer pannexin1 proliferation invasion migration |
| 基金项目: |
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| 中文摘要: |
| 摘 要：［目的］ 探究Pannexin1对乳腺癌细胞MCF-7增殖、侵袭迁移的影响及可能机制。［方法］ MTT法检测0、12.5、25、50、100、200μmol/L甘珀酸（CBX）对乳腺癌细胞MCF-7增殖能力的影响；集落克隆检测CBX对乳腺癌细胞MCF-7增殖能力的影响；采用实时荧光法检测细胞间荧光传递能力；化学发光法检测细胞外ATP浓度。使用100μmol/LCBX处理乳腺癌细胞MCF-7后，Transwell法检测MCF-7细胞的侵袭迁移能力；Western 印迹法检测乳腺癌细胞中相关蛋白p-ERK1/2、ERK1/2、Vimentin、MMP-9蛋白的表达。［结果］ MTT实验结果表明，200μmol/L CBX可显著性抑制MCF-7细胞增殖能力（P<0.05），而100μmol/L CBX对MCF-7细胞增殖能力无显著性抑制作用（P>0.05）。集落克隆实验结果表明，200 μmol/L CBX可显著性抑制MCF-7细胞增殖能力（P<0.05）。实时荧光法和化学发光法实验结果表明，100μmol/L和200μmol/L CBX显著性抑制Pannexin1通道功能（P<0.05）；Transwell实验和划痕实验结果表明，100μmol/L CBX显著性抑制乳腺癌MCF-7细胞侵袭迁移能力（P<0.05）；Western印迹法结果表明，CBX抑制Pannexin1后，乳腺癌细胞MCF-7中ERK1/2、p-ERK1/2、MMP-9和Vimentin蛋白表达量下降（P<0.05）。［结论］ 200μmol/L CBX抑制Pannexin1通道后，乳腺癌MCF-7细胞活性和增殖能力减弱，而100μmol/L CBX可降低乳腺癌MCF-7细胞侵袭迁移能力，其机制可能与ERK1/2表达量下降有关。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effect and mechanism of pannexin1(Panx-1) channel on proliferation，invasion and migration of breast cancer MCF-7 cells. ［Methods］ Breast cancer MCF-7 cells were treated with different concentrations of Panx-1 channel inhibitor carbenoxolone(CBX)(0，12.5，25，50，100，200 μmol/L). Cell proliferation was detected by MTT assay and colony cloning assay. The intercellular fluorescence transmission ability was detected by real-time fluorescence assay，and the extracellular ATP concentration was measured by chemiluminescence assay. Transwell method was used to detect the invasion and migration ability of MCF-7 cells. Western blotting was used to detect the expression of Panx-1 channel related proteins p-ERK1/2，ERK1/2，vimentin and MMP-9 in breast cancer cells. ［Results］ MTT assay showed that 200μmol/L CBX significantly inhibited the viability of MCF-7 cells(P<0.05)，while 100μmol/L CBX had no significant inhibitory effect on MCF-7 cells(P>0.05). The results of colony cloning experiments showed that 200μmol/L CBX significantly inhibited the proliferation of MCF-7 cells(P<0.05)，while 100μmol/L CBX had no significant inhibitory effect on MCF-7 cells(P>0.05). Real-time fluorescence and chemiluminescence experiments showed that 100μmol/L and 200μmol/L CBX significantly inhibited pannexin1 channel function(P<0.05). Transwell assay and scratch test results showed that 100μmol/L CBX significantly inhibited the invasion and migration ability of MCF-7 cells(P<0.05). Western blotting results showed that the expression of ERK1/2，p-ERK1/2，MMP-9 and Vimentin in breast cancer MCF-7 cells was decreased after CBX treatment(P<0.05). ［Conclusion］ Inhibiting pannexin1 channel by 200μmol/L CBX，the viability and proliferation of breast cancer MCF-7 cells are decreased，while 100μmol/L CBX can reduce the invasion and migration ability of breast cancer MCF-7 cells，which may be related to the expression of ERK1/2 proteins. |
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