| 姜志明,王 增,覃 晶.冰片逆转小细胞肺癌顺铂耐药的研究[J].肿瘤学杂志,2020,26(3):204-209. |
| 冰片逆转小细胞肺癌顺铂耐药的研究 |
| Borneol Reverses Resistance to Cisplatin in Small Cell Lung Cancer Cells |
| 投稿时间：2019-11-30 |
| DOI：10.11735/j.issn.1671-170X.2020.03.B008 |
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| 中文关键词: 小细胞肺癌 冰片 顺铂 耐药 |
| 英文关键词:small cell lung cancer borneol cisplatin resistance |
| 基金项目:浙江省自然科学基金（LY20H290003；LQ18H290002）；浙江省中医药科技计划（2015ZA037） |
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| 中文摘要: |
| 摘 要：［目的］ 评价冰片对小细胞肺癌（SCLC）顺铂（DDP）耐药的逆转作用及其对P糖蛋白（P-glycoprotein，P-gp）和小凹蛋白-1 (Caveolin-1)表达的影响。［方法］ 利用前期建立的SCLC DDP耐药细胞株LTEP-P/DDP，四甲基偶氮唑盐微量酶反应比色法（MTT）检测冰片和/或DDP的抗肿瘤活性，流式细胞仪检测细胞周期和凋亡率，Western blotting检测P-gp和Caveolin-1的表达。［结果］冰片联合DDP对LTEP-P/DDP-0.75 细胞的增殖抑制作用较单用DDP显著性增加（P<0.05），耐药逆转指数为2.246。LTEP-P/DDP-0.75细胞经冰片联合DDP处理细胞后，较单用DDP处理组的G0/G1期和S期降低，G2/M期升高，差异有显著性（P<0.05）。冰片和DDP联合处理后，LTEP-P/DDP-0.75细胞凋亡显著性上升（P<0.05），LTEP-P/DDP-0.75细胞Caveolin-1相比LTEP-P细胞显著性上升（P<0.05），冰片或DDP单独用药组Caveolin-1显著性变化，冰片联合联合DDP组相比对照组能显著性降低LTEP-P/DDP-0.75细胞Caveolin-1表达（P<0.05），而对P-gp无显著性抑制作用。［结论］ 冰片能在一定程度上逆转LTEP-P/DDP-0.75对DDP的耐药，其作用可能与抑制Caveolin-1表达有关。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effect of borneol on resistance to cisplatin(DDP) in small cell lung cancer（SCLC) cells. ［Methods］ DDP-resistant LTEP-P/DDP-0.75 cells were treated with borneol and/or DDP. The cell proliferation was examined by MTT assay，cell cycle and apoptotic rate were detected by flow cytometric analysis，the expressions of P-gp and Caveolin-1 were evaluated by Western blot method.［Results］ The inhibitory effect of borneol combined with DDP on the proliferation of LTEP-P/DDP-0.75 cells was significantly higher than that of DDP alone(P<0.05)，and the reverse index of drug resistance was 2.246. Compared with LTEP-P/DDP-0.75 cells treated with DDP alone，LTEP-P/DDP-0.75 cells treated with borneol combined with DDP significantly decreased in G0/G1 and S phases while increased in G2/M phase(P<0.05). Apoptosis of LTEP-P/DDP-0.75 cells was promoted remarkably after treated with borneol plus DDP. Expression of caveolin-1 in LTEP-P/DDP-0.75 cells was significantly higher than that in LTEP-P cells(P<0.05)，but there were no significant changes in LTEP-P/DDP-0.75 cells treated with borneol or DDP alone. The expression of caveolin-1 in LTEP-P/DDP-0.75 cells treated by borneol combined with DDP was significantly lower than that of the control group(P<0.05). However，addition of borneol did not alter the expression of P-gp in LTEP-P/DDP-0.75 cells. ［Conclusion］ Borneol can partly reverse the resistance of LTEP-P/DDP-0.75 cells to DDP，and it may be related to the inhibition of caveolin-1. |
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