| 田雯钰,林宇宁,梁 莹.NDV-HN致人肺腺癌A549细胞凋亡的实验研究[J].肿瘤学杂志,2020,26(3):198-203. |
| NDV-HN致人肺腺癌A549细胞凋亡的实验研究 |
| NDV-HN Induces Apoptosis of Human Lung Adenocarcinoma A549 Cells |
| 投稿时间：2019-07-15 |
| DOI：10.11735/j.issn.1671-170X.2020.03.B007 |
|
 |
| 中文关键词: 新城疫病毒 A549细胞 血凝素神经氨酸酶 Bcl-2 细胞凋亡 |
| 英文关键词:Newcastle disease virus A549 cells hemagglutinin neuraminidase Bcl-2 apoptosis |
| 基金项目:国家自然科学基金（81460437）；广西自然科学基金（2018GXNSFDA281043） |
|
| 摘要点击次数: 1542 |
| 全文下载次数: 348 |
| 中文摘要: |
| 摘 要：［目的］ 探讨NDV-HN诱导人肺腺癌A549细胞凋亡的相关机制。［方法］ （1）构建包含绿色荧光素蛋白（GFP）及NDV-HN的慢病毒sfGFP-HN载体，用293V细胞包装慢病毒Lv-sfGFP-HN，用293V细胞进行空载质粒转染组（空载组）慢病毒包装，命名为Lv-sfGFP；（2）以空载组A549-sfGFP细胞和空白组A549为对照，用慢病毒转染方法建立稳定表达NDV-HN蛋白的A549细胞株，即A549-sfGFP-HN细胞株，RT-PCR、Western blot检测A549细胞NDV-HN蛋白的表达；（3）分别在A549-sfGFP-HN和A549两组细胞中加入顺铂，流式细胞术检测细胞凋亡；（4）在基因水平和蛋白水平上分别用RT-PCR和Western blot检测各组细胞凋亡相关蛋白Bcl-2的表达。［结果］ （1）Lv-sfGFP-HN和Lv-sfGFP慢病毒分别转染A549细胞后，只在转染组A549-sfGFP-HN细胞检测到NDV-HN蛋白。（2）转染组A549-sfGFP-HN细胞凋亡率明显高于空白组A549细胞 (P＜0.01)，转染组A549-sfGFP-HN细胞加入顺铂后，其细胞凋亡率明显增加。（3）转染组A549-sfGFP-HN细胞与空载质粒转染组A549细胞和空白组A549细胞相比，凋亡相关蛋白Bcl-2表达量明显下调(P＜0.01)。［结论］ 慢病毒转染NDV-HN到A549细胞后导致A549-sfGFP-HN细胞出现凋亡，且与Bcl-2下调有关。转染NDV-HN的肺癌细胞联合使用抗癌药物顺铂，增加了肺癌细胞凋亡率。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effect of Newcastle disease virus(NDV) -hemagglutinin neuraminidase (HN) on the apoptosis of human lung adenocarcinoma A549 cells. ［Methods］ The lentiviral sfGFP-HN vector containing green luciferin protein(GFP) and NDV-HN was constructed and packaged into lentiviral Lv-sfGFP-HN in 293V cells. The same method was used to package the empty plasmid into 293V cells to get Lv-sfGFP. Lv-sfGFP-HN and Lv-sfGFP were treansfected into A549 cells to form the A549-sfGFP-HN cell line and A549-sfGFP cell line. The A549-sfGFP and A549 cell lines were used as controls. NDV-HN protein was detected by RT-PCR and Western blot in three cell lines. Cisplatin was added to A549-sfGFP-HN and A549 cells，and the apoptosis was detected by flow cytometry. The expression of Bcl-2 mRNA and protein was detected by RT-PCR and Western blot，respectively. ［Results］ After Lv-sfGFP-HN and Lv-sfGFP lentivirus were transfected into A549 cells，NDV-HN protein was detected only in A549-sfGFP-HN cells. The apoptosis rate of A549-sfGFP-HN cells was significantly higher than that in A549 cells(P<0.01). Apoptosis rate increased after A549-sfGFP-HN cells were combined with cisplatin. Expression of Bcl-2 was significantly down-regulated in A549-sfGFP-HN cells，compared with A549 cells and A549-sfGFP A549 cells(P<0.01). ［Conclusions] Newcastle disease virus- hemagglutinin neuraminidase can induce apoptosis of human lung edenocarcinoma cells，the effect is enhanced by cisplatin. |
|
在线阅读
查看全文 查看/发表评论 下载PDF阅读器 |