| 戚 岳,李刚强,濮亚斌.大肠侧向发育型肿瘤中Wnt/β-catenin/c-myc轴激活水平研究[J].肿瘤学杂志,2020,26(2):2-128. |
| 大肠侧向发育型肿瘤中Wnt/β-catenin/c-myc轴激活水平研究 |
| Activation of Wnt/β-catenin/c-myc Axis in Laterally Spreading Colorectal Tumor |
| 投稿时间：2019-03-21 |
| DOI：10.11735/j.issn.1671-170X.2020.02.B010 |
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| 中文关键词: 大肠侧向发育型肿瘤 Wnt/β-catenin/c-myc 隆起型腺瘤 大肠肿瘤 |
| 英文关键词:laterally spreading tumors Wnt/β-catenin/c-myc signal pathway protuberate adenoid tumors colorectal cancers |
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| 中文摘要: |
| 摘 要：［目的］ 研究大肠侧向发育型肿瘤（LSTs）的发生与Wnt/β-catenin/c-myc通路活化的关系。［方法］ 选取20例大肠隆起型腺瘤（PAs）、20例LSTs和20例大肠癌（colorectal cancers，CCs）患者的组织标本，采用免疫组化染色法检测标本中Wnt/β-catenin/c-myc蛋白的表达，反转录PCR（RT-PCR）和Western blot法分别检测标本中对应mRNA和蛋白的相对表达水平。［结果］ 免疫组化染色显示LSTs组中Wnt/β-catenin/c-myc蛋白的阳性表达率(70.0%，75.0%，75.0%)低于CCs组(85.0%，80.0%，80.0%)，但高于PAs组(25.0%，35.0%，40.0%)，差异有统计学意义（P＜0.05）。标本中Wnt/β-catenin/c-myc的mRNA和蛋白水平的变化也与免疫组化结果的趋势一致，差异有统计学意义（P＜0.05）。［结论］ Wnt/β-catenin/c-myc的表达水平可能与大肠肿瘤的恶化相关，这种表达水平的变化可能与LSTs的发生及恶化生物学行为有关。 |
| 英文摘要: |
| Abstract：［Objective］ To study the relationship between the occurrence of and the activation of Wnt/β-catenin/c-myc pathway in laterally spreading colorectal tumor(LST). ［Methods］ Tissue samples of 20 cases of colorectal protruding adenoma(PA)，20 cases of LSTs and 20 cases of colorectal carcinoma(CC) were obtained. The positive expression of Wnt/β-catenin/c-myc protein was detected by immunohistochemical staining. The relative expression levels of corresponding mRNA and protein were detected by reverse transcription PCR(RT-PCR) and Western blot，respectively. ［Results］ Immunohistochemical staining showed that the positive expression of Wnt/β-catenin/c-myc protein in LST group(70.0%，75.0%，75.0%) was lower than that in CC group(85.0%，80.0%，80.0%)，but higher than that in PA group(25.0%，35.0%，40.0%)，and the expression intensity was in the middle position of the three groups(all P<0.05). The changes of mRNA and protein levels of Wnt/β-catenin/c-myc in samples were also consistent with the those of immunohistochemical results(all P<0.05). ［Conclusion] The expression level of Wnt/β-catenin/c-myc may be positively correlated with the deterioration of colorectal tumors，which may be closely related to the malignant biological behavior of LSTs. |
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