| 田 雯,陈 珑,马艳华.血清miR-221-3p及miR-133a-3p在结肠癌中的表达及其临床意义[J].肿瘤学杂志,2020,26(2):2-123. |
| 血清miR-221-3p及miR-133a-3p在结肠癌中的表达及其临床意义 |
| Expression of Serum miR-221-3p and miR-133a-3p in Patients with Colon Cancer and Its Clinical Significance |
| 投稿时间：2019-05-22 |
| DOI：10.11735/j.issn.1671-170X.2020.02.B009 |
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| 中文关键词: 结肠肿瘤 miR-221-3p miR-133a-3p 诊断 |
| 英文关键词:colon cancer miR-221-3p miR-133a-3p clinical significance diagnosis |
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| 中文摘要: |
| 摘 要：［目的］ 探讨结肠癌患者血清miR-221-3p及miR-133a-3p的表达及其临床意义。［方法］ 选取收治的160例结肠癌患者（结肠癌组）、108例结肠良性疾病（良性病变组）和60例健康体检正常者（对照组），检测各组血清miR-221-3p及miR-133a-3p表达水平，分析其表达与临床病理特征的关系。应用ROC曲线分析血清miR-221-3p及miR-133a-3p表达水平对结肠癌的诊断价值。Pearson相关分析血清miR-221-3p及miR-133a-3p表达水平的相关性。［结果］ 结肠癌组血清miR-221-3p（3.94±1.08）表达水平明显高于良性病变组和对照组(1.51±0.43，1.42±0.36）（P均<0.001），结肠癌组血清miR-133a-3p表达水平（1.85±0.62）明显低于良性病变组和对照组(3.18±0.86，3.27±0.94）（P均<0.001）。结肠癌患者血清miR-221-3p及miR-133a-3p表达水平与TNM分期、分化程度及淋巴结转移有关（P<0.05）。ROC曲线分析显示，血清miR-221-3p及miR-133a-3p表达水平诊断结肠癌的最佳截断值分别为2.90、2.26，两项联合诊断结肠癌的曲线下面积最大（0.905，95%CI：0.844～0.962），其敏感性和特异度为90.2%和84.5%。相关分析显示结肠癌患者血清miR-24-3p与miR-221表达水平呈负相关（r=-0.742，P<0.01）。［结论］ 结肠癌患者血清miR-221-3p表达水平明显升高，而血清miR-133a-3p表达水平明显降低，两项联合检测对结肠癌诊断具有一定的价值。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the expression and clinical significance of serum miR-221-3p and miR-133a-3p in patients with colon cancer. ［Methods］ One hundred and sixty patients with colon cancer(colon cancer group)，108 patients with benign colon disease(benign lesion group) and 60 healthy subjects(control group) were recruited. The serum miR-221-3p and miR-133a-3p levels were detected by RT-PCR，and their relationship with clinicopathological characteristics were analyzed. The diagnostic value of serum miR-221-3p and miR-133a-3p in colon cancer were analyzed by ROC curve. The correlation between the expression levels of serum miR-221-3p and miR-133a-3p was analyzed by Pearson correlation. ［Results］ The expression level of serum miR-221-3p in colon cancer group(3.94±1.08) was significantly higher than that in benign lesion group and control group(1.51±0.43，1.42±0.36)(all P<0.001). The expression level of serum miR-133a-3p in colon cancer group(1.85±0.62) was significantly lower than that in benign lesion group and control group(3.18±0.86，3.27±0.94)(all P<0.001). The expression levels of serum miR-221-3p and miR-133a-3p in patients with colon cancer were correlated with TNM stage，differentiation degree and lymph node metastasis(P<0.05). ROC curve analysis showed that the optimal cut-off values of serum levels of miR-221-3p and miR-133a-3p for diagnosis of colon cancer were 2.90 and 2.26，respectively. The area under the curve(AUC) of two combined diagnostic methods for colon cancer was the largest(0.905，95% CI：0.844～0.962)，with sensitivity and specificity of 90.2% and 84.5%，respectively. The correlation analysis showed that the serum levels of miR-221-3p and miR-133a-3p were negatively correlated in patients with colon cancer(r=-0.742，P<0.01). ［Conclusion］ The expression of serum miR-221-3p is increased in patients with colon cancer，while the expression of serum miR-133a-3p is decreased. The combined detection of miR-221-3p and miR-133a-3p is of value in the diagnosis of colon cancer. |
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