田 雯,陈 珑,马艳华.血清miR-221-3p及miR-133a-3p在结肠癌中的表达及其临床意义[J].肿瘤学杂志,2020,26(2):2-123.
血清miR-221-3p及miR-133a-3p在结肠癌中的表达及其临床意义
Expression of Serum miR-221-3p and miR-133a-3p in Patients with Colon Cancer and Its Clinical Significance
投稿时间:2019-05-22  
DOI:10.11735/j.issn.1671-170X.2020.02.B009
中文关键词:  结肠肿瘤  miR-221-3p  miR-133a-3p  诊断
英文关键词:colon cancer  miR-221-3p  miR-133a-3p  clinical significance  diagnosis
基金项目:
作者单位
田 雯 海南省第三人民医院 
陈 珑 海南省第三人民医院 
马艳华 海南省第三人民医院 
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中文摘要:
      摘 要:[目的] 探讨结肠癌患者血清miR-221-3p及miR-133a-3p的表达及其临床意义。[方法] 选取收治的160例结肠癌患者(结肠癌组)、108例结肠良性疾病(良性病变组)和60例健康体检正常者(对照组),检测各组血清miR-221-3p及miR-133a-3p表达水平,分析其表达与临床病理特征的关系。应用ROC曲线分析血清miR-221-3p及miR-133a-3p表达水平对结肠癌的诊断价值。Pearson相关分析血清miR-221-3p及miR-133a-3p表达水平的相关性。[结果] 结肠癌组血清miR-221-3p(3.94±1.08)表达水平明显高于良性病变组和对照组(1.51±0.43,1.42±0.36)(P均<0.001),结肠癌组血清miR-133a-3p表达水平(1.85±0.62)明显低于良性病变组和对照组(3.18±0.86,3.27±0.94)(P均<0.001)。结肠癌患者血清miR-221-3p及miR-133a-3p表达水平与TNM分期、分化程度及淋巴结转移有关(P<0.05)。ROC曲线分析显示,血清miR-221-3p及miR-133a-3p表达水平诊断结肠癌的最佳截断值分别为2.90、2.26,两项联合诊断结肠癌的曲线下面积最大(0.905,95%CI:0.844~0.962),其敏感性和特异度为90.2%和84.5%。相关分析显示结肠癌患者血清miR-24-3p与miR-221表达水平呈负相关(r=-0.742,P<0.01)。[结论] 结肠癌患者血清miR-221-3p表达水平明显升高,而血清miR-133a-3p表达水平明显降低,两项联合检测对结肠癌诊断具有一定的价值。
英文摘要:
      Abstract:[Objective] To investigate the expression and clinical significance of serum miR-221-3p and miR-133a-3p in patients with colon cancer. [Methods] One hundred and sixty patients with colon cancer(colon cancer group),108 patients with benign colon disease(benign lesion group) and 60 healthy subjects(control group) were recruited. The serum miR-221-3p and miR-133a-3p levels were detected by RT-PCR,and their relationship with clinicopathological characteristics were analyzed. The diagnostic value of serum miR-221-3p and miR-133a-3p in colon cancer were analyzed by ROC curve. The correlation between the expression levels of serum miR-221-3p and miR-133a-3p was analyzed by Pearson correlation. [Results] The expression level of serum miR-221-3p in colon cancer group(3.94±1.08) was significantly higher than that in benign lesion group and control group(1.51±0.43,1.42±0.36)(all P<0.001). The expression level of serum miR-133a-3p in colon cancer group(1.85±0.62) was significantly lower than that in benign lesion group and control group(3.18±0.86,3.27±0.94)(all P<0.001). The expression levels of serum miR-221-3p and miR-133a-3p in patients with colon cancer were correlated with TNM stage,differentiation degree and lymph node metastasis(P<0.05). ROC curve analysis showed that the optimal cut-off values of serum levels of miR-221-3p and miR-133a-3p for diagnosis of colon cancer were 2.90 and 2.26,respectively. The area under the curve(AUC) of two combined diagnostic methods for colon cancer was the largest(0.905,95% CI:0.844~0.962),with sensitivity and specificity of 90.2% and 84.5%,respectively. The correlation analysis showed that the serum levels of miR-221-3p and miR-133a-3p were negatively correlated in patients with colon cancer(r=-0.742,P<0.01). [Conclusion] The expression of serum miR-221-3p is increased in patients with colon cancer,while the expression of serum miR-133a-3p is decreased. The combined detection of miR-221-3p and miR-133a-3p is of value in the diagnosis of colon cancer.
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