马 明,马 颖,马 玲.miR-125a-5p靶向调控CDKN2B表达抑制肝癌细胞的增殖和转移[J].肿瘤学杂志,2020,26(2):2-112.
miR-125a-5p靶向调控CDKN2B表达抑制肝癌细胞的增殖和转移
Regulates CDKN2B Expression to Inhibit Proliferation and Metastasis of Hepatocellular Carcinoma Cells
投稿时间:2019-02-21  
DOI:10.11735/j.issn.1671-170X.2020.02.B007
中文关键词:  肝肿瘤  miR-125a-5p  CDKN2B
英文关键词:liver cancer  miR-125a-5p  CDKN2B
基金项目:中国新疆维吾尔自治区自治区自然科学基金(2018D03011)
作者单位
马 明 新疆维吾尔自治区人民医院 
马 颖 新疆医科大学第一附属医院 
马 玲 新疆维吾尔自治区胸科医院 
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中文摘要:
      摘 要:[目的] 探讨miR-125a-5p在肝癌细胞中的表达,及其对肝癌细胞增殖和转移的影响。[方法] 采用荧光实时定量PCR(quantitative real-time polymerase chain reaction,qRT-PCR)方法检测肝癌组织与癌旁组织中miR-125a-5p与细胞周期依赖蛋白激酶抑制因子2B(cyclin dependent kinase inhibitor 2B,CDKN2B)的表达水平;通过向肝癌细胞HepG2细胞系中转染miR-125a-5p mimic和inhibitor干扰内源miR-125a-5p的表达,同时检测其靶基因CDKN2B蛋白的表达变化;采用CCK-8 法检测各组肝癌细胞增殖能力的变化,以及划痕实验对HepG2细胞迁移能力进行评估。[结果] 在肝癌患者中,肝癌组织中miR-125a-5p的表达水平(2.16±0.37)显著高于癌旁组织(1.28±0.21),差异有统计学意义(P<0.05);肝癌组织中CDKN2B的表达水平(0.87±0.15)显著低于癌旁组织(1.56±0.28),两者比较差异有统计学意义(P<0.05)。在32例肝癌患者肝癌组织中,CDKN2B表达水平与miR-125a-5p表达呈显著负相关(r=-0.168,P<0.05);HepG2细胞体外转染实验结果发现,降低细胞内源miR-125a-5p的表达时,CDKN2B表达水平显著升高,细胞的增殖活性以及转移能力会被抑制;而上调细胞内源miR-125a-5p的表达时,CDKN2B表达水平显著下降,细胞的增殖活性与转移能力显著提高,相比于对照组差异有统计学意义(P<0.05)。[结论] miR-125a-5p在肝癌组织中高表达,其可能靶向调控CDKN2B的表达抑制肝癌细胞的增殖活性与转移能力,在肝癌的发生发展过程中起着重要的生理功能。
英文摘要:
      Abstract:[Objective] To investigate the effect of miR-125a-5p on proliferation and metastasis of hepatocellular carcinoma cells and its relation with CDKN2B expression. [Methods] The expression levels of miR-125a-5p and CDKN2B in hepatocellular carcinoma tissues and adjacent tissues were detected by qRT-PCR. Mimic and inhibitor interfere with the expression of endogenous microRNAs-125a-5p were performed by transfecting microRNAs into hepatocellur carcinoma HepG2 cells,then the expression of CDKN2B protein was detected. The proliferation of hepatoma cells was detected by CCK-8. Scratch test was used to evaluate the migration ability of HepG2 cells. [Results] In patients with hepatocellular carcinoma,the expression level of microRNA-125a-5p in cancer tissues(2.16±0.37) was significantly higher than that in adjacent tissues(1.28±0.21)(P<0.05),and the expression level of CDKN2B in cancer tissues(0.87±0.15) was significantly lower than that in adjacent tissues(1.56±0.28)(P<0.05). Pearson correlation analysis showed that the expression of CDKN2B was negatively correlated with the expression of miR-125a-5p(r=-0.168,P<0.05). The results of in vitro transfection of HepG2 cells showed that the expression of CDKN2B increased significantly when the expression of endogenous microRNAs-125a-5p was inhibited,and the proliferation and migration ability of HepG2 cells were inhibited. When the expression of endogenous microRNAs-125a-5p was up-regulated,the expression of CDKN2B decreased significantly,and the proliferation and migration ability of HepG2 cells increased significantly(P<0.05). [Conclusion] The miR-125a-5p is highly expressed in HCC tissues,which may promote the proliferation and metastasis of HCC cells through regulation of CDKN2B expression.
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