| 马 明,马 颖,马 玲.miR-125a-5p靶向调控CDKN2B表达抑制肝癌细胞的增殖和转移[J].肿瘤学杂志,2020,26(2):2-112. |
| miR-125a-5p靶向调控CDKN2B表达抑制肝癌细胞的增殖和转移 |
| Regulates CDKN2B Expression to Inhibit Proliferation and Metastasis of Hepatocellular Carcinoma Cells |
| 投稿时间：2019-02-21 |
| DOI：10.11735/j.issn.1671-170X.2020.02.B007 |
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| 中文关键词: 肝肿瘤 miR-125a-5p CDKN2B |
| 英文关键词:liver cancer miR-125a-5p CDKN2B |
| 基金项目:中国新疆维吾尔自治区自治区自然科学基金（2018D03011） |
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| 中文摘要: |
| 摘 要：［目的］ 探讨miR-125a-5p在肝癌细胞中的表达，及其对肝癌细胞增殖和转移的影响。［方法］ 采用荧光实时定量PCR(quantitative real-time polymerase chain reaction，qRT-PCR)方法检测肝癌组织与癌旁组织中miR-125a-5p与细胞周期依赖蛋白激酶抑制因子2B（cyclin dependent kinase inhibitor 2B，CDKN2B）的表达水平；通过向肝癌细胞HepG2细胞系中转染miR-125a-5p mimic和inhibitor干扰内源miR-125a-5p的表达，同时检测其靶基因CDKN2B蛋白的表达变化；采用CCK-8 法检测各组肝癌细胞增殖能力的变化，以及划痕实验对HepG2细胞迁移能力进行评估。［结果］ 在肝癌患者中，肝癌组织中miR-125a-5p的表达水平（2.16±0.37）显著高于癌旁组织（1.28±0.21），差异有统计学意义（P<0.05）；肝癌组织中CDKN2B的表达水平（0.87±0.15）显著低于癌旁组织（1.56±0.28），两者比较差异有统计学意义（P<0.05）。在32例肝癌患者肝癌组织中，CDKN2B表达水平与miR-125a-5p表达呈显著负相关（r=-0.168，P<0.05）；HepG2细胞体外转染实验结果发现，降低细胞内源miR-125a-5p的表达时，CDKN2B表达水平显著升高，细胞的增殖活性以及转移能力会被抑制；而上调细胞内源miR-125a-5p的表达时，CDKN2B表达水平显著下降，细胞的增殖活性与转移能力显著提高，相比于对照组差异有统计学意义（P<0.05）。［结论］ miR-125a-5p在肝癌组织中高表达，其可能靶向调控CDKN2B的表达抑制肝癌细胞的增殖活性与转移能力，在肝癌的发生发展过程中起着重要的生理功能。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effect of miR-125a-5p on proliferation and metastasis of hepatocellular carcinoma cells and its relation with CDKN2B expression. ［Methods］ The expression levels of miR-125a-5p and CDKN2B in hepatocellular carcinoma tissues and adjacent tissues were detected by qRT-PCR. Mimic and inhibitor interfere with the expression of endogenous microRNAs-125a-5p were performed by transfecting microRNAs into hepatocellur carcinoma HepG2 cells，then the expression of CDKN2B protein was detected. The proliferation of hepatoma cells was detected by CCK-8. Scratch test was used to evaluate the migration ability of HepG2 cells. ［Results］ In patients with hepatocellular carcinoma，the expression level of microRNA-125a-5p in cancer tissues(2.16±0.37) was significantly higher than that in adjacent tissues(1.28±0.21)(P<0.05)，and the expression level of CDKN2B in cancer tissues(0.87±0.15) was significantly lower than that in adjacent tissues(1.56±0.28)(P<0.05). Pearson correlation analysis showed that the expression of CDKN2B was negatively correlated with the expression of miR-125a-5p(r=-0.168，P<0.05). The results of in vitro transfection of HepG2 cells showed that the expression of CDKN2B increased significantly when the expression of endogenous microRNAs-125a-5p was inhibited，and the proliferation and migration ability of HepG2 cells were inhibited. When the expression of endogenous microRNAs-125a-5p was up-regulated，the expression of CDKN2B decreased significantly，and the proliferation and migration ability of HepG2 cells increased significantly(P<0.05). ［Conclusion］ The miR-125a-5p is highly expressed in HCC tissues，which may promote the proliferation and metastasis of HCC cells through regulation of CDKN2B expression. |
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