| 贾萌萌,王许艳,陈 健.血清miR-15b诊断子宫内膜癌及预判淋巴结转移的价值分析[J].肿瘤学杂志,2020,26(1):43-46. |
| 血清miR-15b诊断子宫内膜癌及预判淋巴结转移的价值分析 |
| Value of Serum miR-15b in Diagnosis of Endometrial Carcinoma and Prediction of Lymphatic Metastasis |
| 投稿时间：2019-07-08 |
| DOI：10.11735/j.issn.1671-170X.2020.01.B010 |
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| 中文关键词: 子宫内膜腺癌 淋巴结转移 HE4 CA125 miR-15b |
| 英文关键词:endometrial adenocarcinoma lymph node metastasis HE4 CA125 miR-15b |
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| 中文摘要: |
| 摘 要：［目的］ 探讨血清miR-15b用于诊断子宫内膜腺癌的价值及其对淋巴结转移的预判能力。［方法］ 选取21例我院收治并诊断为子宫内膜腺癌及13例无生殖系统疾病的健康女性，采用酶联吸附法检测血清CA125、HE4水平，应用实时荧光定量技术对血清中miR-15b的水平进行相对定量。利用相关和回归分析对miR-15b指标与CA125、HE4做关联探讨；对子宫内膜腺癌患者淋巴结转移情况与miR-15b变量值进行ROC分析，寻找最佳的截断值。［结果］ 子宫内膜腺癌患者的血清CA125、HE4和miR-15b含量均高于对照组，差异有统计学意义。CA125与HE4、HE4与miR-15b之间存在线性正相关关系。 CA125在FIGO各期中比较无差异，HE4和miR-15b在FIGO的各期均存在差异。当血清miR-15b水平相对于正常健康人群高出3倍，此时诊断淋巴转移的敏感度为90.91%，特异性为60%。［结论］ 血清HE4与miR-15b可联合诊断子宫内膜腺癌，且miR-15b在子宫内膜腺癌的FIGO病理分期中存在差异，在判断肿瘤淋巴结转移方面有较高的价值。 |
| 英文摘要: |
| Abstract：［Objective］ To explore the value of serum miR-15b in the diagnosis of endometrial adenocarcinomas and prediction lymph node metastasis. ［Methods］ Twenty-one patients with endometrial adenocarcinoma admitted in the First Affiliated Hospital of Henan University of Science and Technology and 13 healthy women were enrolled in the study. Serum levels of carbohydrate antigen 125(CA125) and human epididymisprotein4(HE4) were detected by ELISA，serum miR-15b was detected by RT-qPCR. Correlation and regression analysis were used to explore the correlation of miR-15b and CA125 and HE4. ROC(receiver operating characteristic) analysis was applied to predict the lymphatic metastasis of patients with endometrial adenocarcinoma. ［Results］ The serum levels of CA125，HE4 and miR-15b in patients with endometrial adenocarcinoma were all higher than those in the control group(P<0.05). Correlation analysis showed that CA125 was positively correlated with HE4 and HE4 was positively correlated with miR-15b. CA125 showed no differences in each phase of FIGO，while HE4 and miR-15b showed differences in each phase of FIGO. When the serum miR-15b level was three times higher than that of healthy women(cut-off value=3)，the sensitivity to diagnose lymphatic metastasis was 90.91% and specificity was 60%. ［Conclusion］ Serum HE4 and miR-15b might be used as indicators for the diagnosis of endometrial adenocarcinoma，and the prediction of lymph node metastasis. |
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