| 周 燕,张洁霞.hsa-miR-23a-3p在肺腺癌组织中的表达及意义[J].肿瘤学杂志,2020,26(1):31-34. |
| hsa-miR-23a-3p在肺腺癌组织中的表达及意义 |
| Expression and Clinical Significance of hsa-miR-23a-3p in Lung Adenocarcinoma |
| 投稿时间：2019-04-01 |
| DOI：10.11735/j.issn.1671-170X.2020.01.B007 |
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| 中文关键词: hsa-miR-23a-3p 肺腺癌 临床意义 |
| 英文关键词:hsa-miR-23a-3p lung adenocarcinoma clinical significance |
| 基金项目:广州医科大学附属第一医院2015年度科研项目临床应用与转化医学项目（201515gyfyy） |
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| 中文摘要: |
| 摘 要：［目的］ 探讨hsa-miR-23a-3p在肺腺癌织中的表达水平及临床意义。［方法］ 收集我院呼吸科就诊的42例肺腺癌患者的癌组织及癌旁组织样本，同时选取37例非肺腺癌患者的肺穿刺样本为对照组。采用荧光定量PCR法检测各组患者肺组织中hsa-miR-23a-3p相对表达水平，分析hsa-miR-23a-3p在肺腺癌组织中的表达及与患者临床特征的关系；以患者血清癌胚抗原(CEA)指标为参考，利用受试者工作特征曲线(ROC)评估肺腺癌患者癌组织中hsa-miR-23a-3的表达临床应用价值。［结果］ 对照组与癌旁组织中hsa-miR-23a-3p的表达水平差异无统计学意义(4.32±0.34 vs 4.16±0.41，P>0.05)，与对照组与癌旁组织比，肺腺癌组织hsa-miR-23a-3p表达较高(6.08±0.47 vs 4.32±0.34，6.08±0.47 vs 4.16±0.41，P<0.01)。不同性别(6.16±0.82 vs 5.89±0.73)、分化程度(6.20±0.84 vs 5.97±0.67)癌组织的hsa-miR-23a-3p表达差异无统计学意义(P＞0.05)，TNM分期越高(5.13±0.49 vs 6.26±0.51)、肿瘤越大(6.47±0.65 vs 5.94±0.71)、淋巴结转移(6.32±0.53 vs 5.83±0.46)及CEA阳性(6.47±0.44 vs 5.79±0.41)癌组织的hsa-miR-23a-3p表达显著性增高(P<0.001)。ROC曲线分析显示，与CEA比，hsa-miR-23a-3p的曲线下面积高(0.779 vs 0.683)。［结论］ 肺腺癌组织hsa-miR-23a-3p呈高表达，与淋巴结转移、肿瘤大小及TNM分期相关，可用于辅助诊断肺腺癌。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the expression and clinical significance of hsa-miR-23a-3p in lung adenocarcinoma. ［Methods］ The cancer tissue samples and paracancerous tissue samples of 42 patients with lung adenocarcinoma admitted to our hospital were collected. The lung puncture samples of 37 patients with non-lung adenocarcinoma were obtained as the control group. The expression of hsa-miR-23a-3p in tissue samples was detected by RT-PCR. The relationship between the expression of hsa-miR-23a-3p in lung adenocarcinoma and clinical characteristics of patients was analyzed. The serum carcinoembryonic antigen(CEA) levels of patients were measured. The receiver operating characteristic(ROC) was applied to evaluate the value of hsa-miR-23a-3p in diagnosis of lung adenocarcinoma. ［Results］ There were no significant differences in the expression level of hsa-miR-23a-3p between the control group and the paracancerous tissues(4.32±0.34 vs 4.16±0.41，P>0.05). Compared with the control group and paracancerous tissues，the expression of hsa-miR-23a-3p in lung adenocarcinoma tissue was higher(6.08±0.47 vs 4.32±0.34，6.08±0.47 vs 4.16±0.41，P<0.05). There was no significant difference in the expression of hsa-miR-23a-3p between male and female patients(6.16±0.82 vs 5.89±0.73) or patients with different differentiation of cancer tissue(6.20±0.84 vs 5.97±0.67)(P>0.05). The patients with higher TNM stage(5.13±0.49 vs 6.26±0.51)，larger tumor size(6.47±0.65 vs 5.94±0.71)，positive lymph node metastasis(6.32±0.53 vs 5.83±0.46)，or positive serum CEA level(6.47±0.44 vs 5.79±0.41) had higher expression of hsa-miR-23a-3p(all P<0.05). ROC curve analysis showed that the area under the curve(0.779 vs 0.683) and sensitivity(71.4% vs 47.6%) of hsa-miR-23a-3p were higher than CEA. ［Conclusion］ The hsa-miR-23a-3p is highly expressed in patients with lung adenocarcinoma and is associated with lymph node metastasis，tumor size and TNM staging，indicating that it might be used the diagnosis of lung adenocarcinoma. |
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