| 陈金良,单国进,孙才兴.银杏叶提取物通过调控NF-κB信号通路抑制神经胶质瘤U87细胞增殖和侵袭[J].肿瘤学杂志,2020,26(1):26-30. |
| 银杏叶提取物通过调控NF-κB信号通路抑制神经胶质瘤U87细胞增殖和侵袭 |
| Ginkgo Biloba Extract Inhibits Proliferation and Invasion of Glioblastoma U87 Cells Through Regulating NF-κB Signaling Pathway |
| 投稿时间：2019-04-21 |
| DOI：10.11735/j.issn.1671-170X.2020.01.B006 |
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| 中文关键词: 银杏叶提取物 神经胶质瘤 增殖 侵袭 |
| 英文关键词:extract of gingko biloba glioblastoma proliferation invasion |
| 基金项目:浙江省医药卫生科技项目（2017KY260）；浙江省医药卫生科技项目（2019RC127）；浙江省中管局项目（2016ZA039）；浙江省医药卫生科技项目（2018KY291，2018KY292） |
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| 中文摘要: |
| 摘 要：［目的］ 评价银杏叶提取物(EGb761)对神经胶质瘤U87细胞的生长与侵袭的影响，并进一步分析EGb761对U87细胞作用的分子机制。［方法］ 体外培养人神经胶质瘤U87细胞，给予不同浓度(0、100、200、400mg/L)银杏叶提取物干预（24h、48h、72h）后，采用CCK-8技术检测EGb761对U87细胞增殖的影响。Transwell小室和流式细胞凋亡技术分析EGb761对U87细胞侵袭和凋亡的影响；Western blot检测EGb761处理后U87细胞内NF-κB与cyclin D1、 iNOS和COX-2蛋白的表达变化。［结果］ 银杏叶提取物可有效抑制神经胶质瘤U87细胞的增殖和侵袭，促进细胞凋亡；Western blot提示EGb761干预的U87细胞内NF-κB 与cyclin D1、 iNOS、COX-2蛋白的表达均明显下调。［结论］ EGb761抑制神经胶质瘤U87细胞的生长增殖和侵袭能力，其可能是通过NF-κB信号通路而发挥抗肿瘤作用的。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effect of Ginkgo biloba extract(EGb761) on the growth and invasion of glioma U87 cells and the molecular mechanism. ［Methods］ U87 cells were cultured in vitro and treated with EGb761 of different concentrations(0，100，200，400mg/L) for different time(24h，48h，72h). The proliferation of U87 cells was detected with CCK-8 assay. Transwell migration assay and flow cytometry were employed to determine the effect of EGb761 on invasion and apoptosis of U87 cells，respectively. Changes of NF-κB，cyclin D1，iNOS and COX-2 expressions in U87 cells were detected by Western Blot. ［Results］ EGb761 inhibited the proliferation and invasion of U87 cells and promoted its apoptosis. Western blot revealed that EGb761-treated U87 cells significantly down-regulated the expression of NF-κB，cyclin D1，iNOS and COX-2. ［Conclusion］ EGb761 can significantly inhibit the proliferation and invasion of U87 cells，which is associated with the regulation of NF-κB signaling pathway. |
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