| 茹利新,钟 婧,徐佳敏.LncRNA-HEIH/EZH2通路在肝癌中的作用及血清学表达意义[J].肿瘤学杂志,2019,25(12):1075-1079. |
| LncRNA-HEIH/EZH2通路在肝癌中的作用及血清学表达意义 |
| Expression of LncRNA-HEIH/EZH2 in Liver Cancer Patients and Its Relation with Tumor Cell Proliferation |
| 投稿时间：2019-07-06 |
| DOI：10.11735/j.issn.1671-170X.2019.12.B010 |
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| 中文关键词: lncRNA-HEIH EZH2 肝肿瘤 |
| 英文关键词:lncRNA-HEIH EZH2 liver cancer |
| 基金项目:湖州市科技局资助项目（2015C102096；2014GYB02） |
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| 摘要点击次数: 1411 |
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| 中文摘要: |
| 摘 要：［目的］ 探讨long non-coding RNA-HEIH/enhancer of zeste homolog 2(lnc RNA-HEIH/EZH2)通路在肝癌中的作用及血清学表达中的意义。［方法］ 选择确诊为肝癌的早期患者30例、晚期患者30例以及健康志愿者30例，收集其临床资料及血清学样本。运用qPCR方法，检测其血清中的lncRNA-HEIH基因表达量。利用脂质体包裹lncRNA-HEIH反义寡核苷酸片段转染肝癌细胞，构建lncRNA-HEIH基因沉默的BEL-7402肝癌细胞模型，运用qPCR方法检测lncRNA-HEIH基因及EZH2基因在lncRNA-HEIH基因沉默后的表达。运用Western Blotting对肝癌细胞中EZH2蛋白进行半定量检测。运用CCK-8法对 lncRNA-HEIH基因沉默前后肝癌细胞的增殖活性进行检测。［结果］ 早期和晚期肝癌患者血清中lncRNA-HEIH基因及EZH2基因的表达量均高于健康人，差异有统计学意义（P＜0.05）。当lncRNA-HEIH基因沉默后，EZH2基因表达下降，肝癌细胞的增殖活性明显降低。［结论］ LncRNA-HEIH基因可作为肝癌发生发展的潜在的血清学生物标志物。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the expression of lncRNA-HEIH gene in liver cancer patients and its relation with tumor cell proliferation. ［Methods］ Thirty patients with early liver cancer，30 patients with advanced liver cancer and 30 healthy subjects were enrolled in the study. The expression of lncRNA-HEIH gene in serum was detected by qPCR. Liposome-coated lncRNA-HEIH antisense oligonucleotide fragments were transfected into liver cancer BEL-7402 cells to silence lncRNA-HEIH gene，then the expressions of lncRNA-HEIH gene and EZH2 gene were detected. EZH2 protein expression in BEL-7402 cells was detected by Western blot. The proliferation activity of BEL-7402 cells was detected by CCK-8 before and after lncRNA-HEIH gene silencing. ［Results］ The expression of lncRNA-HEIH gene and EZH2 gene in serum of patients with early and advanced liver cancer was higher than that of healthy subjects(P<0.05). When lncRNA-HEIH gene was silenced，the expression of EZH2 gene decreased，and the proliferation activity of liver cancer cells inhibited significantly.［Conclusion］ The expression of lncRNA-HEIH gene is upregulated in liver cancer，which might be used as a potential serological biomarker for liver cancer. |
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