| 高胜男,张明昱,都 兰.EZH2在恶性胸膜间皮瘤中的作用机制研究[J].肿瘤学杂志,2019,25(12):1070-1074. |
| EZH2在恶性胸膜间皮瘤中的作用机制研究 |
| A Mechanism of EZH2 in Malignant Pleural Mesothelioma |
| 投稿时间：2018-09-09 |
| DOI：10.11735/j.issn.1671-170X.2019.12.B009 |
|
 |
| 中文关键词: 恶性胸膜间皮瘤 EZH2 Gli1 Cul4A Hegdehog信号传导通路 |
| 英文关键词:malignant pleural mesothelioma EZH2 Gli1 Cul4A Hegdehog signaling pathway |
| 基金项目:国家自然科学基金（81160253）；内蒙古自治区肿瘤生物协同创新培育中心项目（1619002010）；内蒙古自然科学基金（2017MS0830） |
|
| 摘要点击次数: 1305 |
| 全文下载次数: 0 |
| 中文摘要: |
| 摘 要：［目的］ 探讨恶性胸膜间皮瘤中EZH2、Cul4A、Gli1的表达及其相互作用关系。［方法］ Real-time PCR检测恶性间皮瘤细胞株H2452、MSTO-211H以及人正常间皮细胞株Met-5A中EZH2、Cul4A、Gli1 mRNA的表达量；上调或下调EZH2表达后检测Cul4A、Gli1 mRNA的表达变化；利用免疫组化技术检测恶性胸膜间皮瘤患者病理组织中EZH2、Cul4A、Gli1表达并分析其相关性。［结果］ EZH2高表达的H2452细胞中，Gli1呈高表达，而Cul4A表达量与正常细胞无明显差异，抑制EZH2表达可使Gli1表达量下降，但对Cul4A表达无明显影响。EZH2低表达的MSTO-211H细胞中，Gli1呈低表达，导入外源性EZH2基因，可使Gli1表达升高，而Cul4A表达无明显改变。恶性胸膜间皮瘤标本中，EZH2表达阳性率为77.6%（52/67），Cul4A表达阳性率为76.1%（51/67），Gli1表达阳性率为65.7%（44/67），EZH2与Gli1表达呈正相关（r=0.815，P<0.05），EZH2与Cul4A表达无明显相关性（r=0.145，P>0.05），Cul4A与Gli1表达呈正相关（r=0.577，P<0.05）。［结论］ 恶性胸膜间皮瘤中EZH2、Cul4A、Gli1呈高表达，并且Gli1表达与EZH2表达存在相关性，可能为EZH2的下游调控基因。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the expression of EZH2，Cul4A and Gli1 in malignant pleural mesothelioma (MPM) and their correlations. ［Methods］ The expression of EZH2，Cul4A and Gli1 mRNA in malignant mesothelioma H2452，MSTO-211H cells and human normal mesothelial Met-5A cells was detected by real-time RT-PCR. The expression of Cul4A and Gli1 mRNA was also detected after up-regulating or down-regulating EZH2. The expression of EZH2，Cul4A and Gli1 in tumor tissues was detected by immunohistochemical technique in 67 patients with MPM. The correlations among EZH2，Cul4A and Gli1 expression were analyzed. ［Results］ The expression of EZH2 and Gli1 was higher in H-2452 cells，but there was no difference in Cul4A expression compared with normal mesothelial Met-5A cells. The expression of Gli1 was decreased，but there was no significant change in Cul4A expression after down-regulation of EZH2 expression. The expression of EZH2 and Gli1 was both low in MSTO-211H cells；the expression of Gli1 was increased after introduction of exogenous EZH2 gene in MSTO-211H cells，while the expression of Cul4A had no significant change. In malignant pleural mesothelioma tissues，the positive rate of EZH2 expression was 77.6%(52/67)，Cul4A was 76.1% (51/67)，and Gli1 was 65.7%(44/67). There was a positive correlation between the expression of EZH2 and Gli1(r=0.815，P<0.05)，between Cul4A and Gli1(r=0.577，P<0.05)，but no correlation between EZH2 Cul4A expression(r=0.145，P>0.05). ［Conclusion］ EZH2，Cul4A and Gli1 are highly expressed in MPM，and there is a correlation between Gli1 and EZH2 expression，indicating that Gli1 may be the downstream regulatory gene of EZH2. |
|
在线阅读
查看全文 查看/发表评论 下载PDF阅读器 |