| 李晓文,金海敏,黄 海.CRNDE和miR-181a在结直肠癌中的表达及临床意义[J].肿瘤学杂志,2019,25(12):1049-1053. |
| CRNDE和miR-181a在结直肠癌中的表达及临床意义 |
| Expression and Clinical Significance of CRNDE and MiR-181a in Colorectal Cancer |
| 投稿时间：2019-05-05 |
| DOI：10.11735/j.issn.1671-170X.2019.12.B005 |
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| 中文关键词: 结直肠肿瘤 CRNDE miR-181a 表达 |
| 英文关键词:colorectal cancer CRNDE miR-181a expression |
| 基金项目:浙江省杭州市科委项目( 20170533B87) |
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| 中文摘要: |
| 摘 要：［目的］ 研究结直肠癌癌组织中长链非编码RNA（long-noncoding RNA，LncRNA）CRNDE及微小RNA（microRNA，miR）-181a表达及其临床意义。［方法］ 应用荧光实时定量PCR（quantitative real-time PCR，qRT-PCR）检测89例结直肠癌癌组织和癌旁组织中CRNDE及miR-181a的表达，分析组间CRNDE及miR-181a表达差异及两者表达与临床病理特征的关系。Kaplan-Meier生存分析比较不同水平CRNDE、miR-181a患者3年总体生存率（OS）的差异。［结果］ 与癌旁组织相比，结直肠癌组织中CRNDE表达水平明显升高，miR-181a表达水平明显降低（P均<0.05）。结直肠癌癌组织中CRNDE、miR-181a表达与肿瘤分期、肿瘤分化有关（P均<0.05），与性别、年龄、肿瘤大小、肿瘤位置及淋巴结转移无关（P均>0.05）。癌组织中CRNDE与miR-181a表达呈显著负相关（r=-0.558，P=0.008）。Kaplan-Meier分析表明癌组织中CRNDE高表达患者3年OS明显低于CRNDE低表达者（P<0.05），而高表达miR-181a与低表达miR-181a患者3年OS无明显差异（P>0.05）。［结论］ 结直肠癌组织中CRNDE表达升高，而miR-181a表达降低，两者均参与结直肠癌的发生发展过程，有可能成为新的肿瘤标志物。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the expression and clinical significance of long non-coding RNA(LncRNA) CRNDE and microRNA(miR)-181a in colorectal cancer tissues. ［Methods］ The expression of CRNDE and miR-181a in 89 specimens of colorectal cancer tissues and adjacent tissues were detected by real-time quantitative PCR(qRT-PCR). The differences of CRNDE and miR-181a expression between groups and their relationship with clinicopathological features were analyzed. The 3-year overall survival time(OS) of patients with different levels of CRNDE and miR-181a were compared by Kaplan-Meier survival analysis. ［Results］ Compared with adjacent tissues，the expression of CRNDE was significantly increased and miR-181a was significantly decreased in colorectal cancer tissues(all P<0.05).The expressions of CRNDE and miR-181a in colorectal cancer tissues were correlated with the tumor stage and tumor differentiation(P<0.05)，and were not correlated with the gender，age，tumor size，tumor location and lymph node metastasis(P>0.05).There was a significant negative correlation between CRNDE and miR-181a in cancer tissues(r=-0.558，P=0.008). Kaplan-Meier analysis showed that the 3-year OS of patients with high CRNDE expression was significantly lower than that of patients with low CRNDE expression(P<0.05)，while there was no significant difference in 3 years OS between patients with high miR-181a expression and low miR-181a expression(P>0.05). ［Conclusion］ The expression of CRNDE is increased in colorectal cancer tissues，while the expression of miR-181a is decreased. Both of them may be involved in the development of colorectal cancer and might be used as new tumor markers. |
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