李晓文,金海敏,黄 海.CRNDE和miR-181a在结直肠癌中的表达及临床意义[J].肿瘤学杂志,2019,25(12):1049-1053. |
CRNDE和miR-181a在结直肠癌中的表达及临床意义 |
Expression and Clinical Significance of CRNDE and MiR-181a in Colorectal Cancer |
投稿时间:2019-05-05 |
DOI:10.11735/j.issn.1671-170X.2019.12.B005 |
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中文关键词: 结直肠肿瘤 CRNDE miR-181a 表达 |
英文关键词:colorectal cancer CRNDE miR-181a expression |
基金项目:浙江省杭州市科委项目( 20170533B87) |
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中文摘要: |
摘 要:[目的] 研究结直肠癌癌组织中长链非编码RNA(long-noncoding RNA,LncRNA)CRNDE及微小RNA(microRNA,miR)-181a表达及其临床意义。[方法] 应用荧光实时定量PCR(quantitative real-time PCR,qRT-PCR)检测89例结直肠癌癌组织和癌旁组织中CRNDE及miR-181a的表达,分析组间CRNDE及miR-181a表达差异及两者表达与临床病理特征的关系。Kaplan-Meier生存分析比较不同水平CRNDE、miR-181a患者3年总体生存率(OS)的差异。[结果] 与癌旁组织相比,结直肠癌组织中CRNDE表达水平明显升高,miR-181a表达水平明显降低(P均<0.05)。结直肠癌癌组织中CRNDE、miR-181a表达与肿瘤分期、肿瘤分化有关(P均<0.05),与性别、年龄、肿瘤大小、肿瘤位置及淋巴结转移无关(P均>0.05)。癌组织中CRNDE与miR-181a表达呈显著负相关(r=-0.558,P=0.008)。Kaplan-Meier分析表明癌组织中CRNDE高表达患者3年OS明显低于CRNDE低表达者(P<0.05),而高表达miR-181a与低表达miR-181a患者3年OS无明显差异(P>0.05)。[结论] 结直肠癌组织中CRNDE表达升高,而miR-181a表达降低,两者均参与结直肠癌的发生发展过程,有可能成为新的肿瘤标志物。 |
英文摘要: |
Abstract:[Objective] To investigate the expression and clinical significance of long non-coding RNA(LncRNA) CRNDE and microRNA(miR)-181a in colorectal cancer tissues. [Methods] The expression of CRNDE and miR-181a in 89 specimens of colorectal cancer tissues and adjacent tissues were detected by real-time quantitative PCR(qRT-PCR). The differences of CRNDE and miR-181a expression between groups and their relationship with clinicopathological features were analyzed. The 3-year overall survival time(OS) of patients with different levels of CRNDE and miR-181a were compared by Kaplan-Meier survival analysis. [Results] Compared with adjacent tissues,the expression of CRNDE was significantly increased and miR-181a was significantly decreased in colorectal cancer tissues(all P<0.05).The expressions of CRNDE and miR-181a in colorectal cancer tissues were correlated with the tumor stage and tumor differentiation(P<0.05),and were not correlated with the gender,age,tumor size,tumor location and lymph node metastasis(P>0.05).There was a significant negative correlation between CRNDE and miR-181a in cancer tissues(r=-0.558,P=0.008). Kaplan-Meier analysis showed that the 3-year OS of patients with high CRNDE expression was significantly lower than that of patients with low CRNDE expression(P<0.05),while there was no significant difference in 3 years OS between patients with high miR-181a expression and low miR-181a expression(P>0.05). [Conclusion] The expression of CRNDE is increased in colorectal cancer tissues,while the expression of miR-181a is decreased. Both of them may be involved in the development of colorectal cancer and might be used as new tumor markers. |
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