| 刘淑娟,金翠红.整合素α2β1和CD44V4与早期宫颈鳞癌淋巴结转移的关系[J].肿瘤学杂志,2019,25(11):972-975. |
| 整合素α2β1和CD44V4与早期宫颈鳞癌淋巴结转移的关系 |
| Relationship Between Integrinα2β1 and CD44V4 and Lymph Node Metastasis in Early Cervical Squamous Cell Carcinoma |
| 投稿时间：2019-03-12 |
| DOI：10.11735/j.issn.1671-170X.2019.11.B009 |
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| 中文关键词: 整合素α2β1 CD44V4 宫颈鳞癌 淋巴结转移 |
| 英文关键词:integrinα2β1 CD44V4 cervical squamous cell carcinoma lymph node metastasis |
| 基金项目:承德市科学技术研究与发展计划项目（201804A002） |
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| 中文摘要: |
| 摘 要：［目的］ 探讨整合素α2β1和CD44V4与早期宫颈鳞癌淋巴结转移的关系。［方法］ 收集我院宫颈鳞癌Ⅰb1期标本81份，正常宫颈组织20份和宫颈鳞状细胞原位癌组织20份。采用实时定量PCR和免疫组化法分别检测组织中整合素α2β1和CD44V4的表达，另外采用免疫组化法检测标记D2-40标记的淋巴管密度（LVD）。采用Pearson分析检验整合素α2β1、CD44V4和LVD相关性。［结果］ 宫颈癌组织中整合素α2β1和CD44V4 mRNA的相对表达量分别为1.1±0.2和1.3±0.1，均高于正常宫颈组织［(0.1±0.0)和（0.1±0.0）］和鳞状细胞原位癌组织［(0.3±0.1)和（0.3±0.1）］（P＜0.05）。宫颈癌组织中整合素α2β1和CD44V4蛋白的阳性表达率分别为87.7%和85.2%，均高于正常组织（5.0%和10.0%）和鳞状细胞原位癌组织（70.0%和40.0%）（P＜0.05）。高中分化宫颈癌患者整合素α2β1和CD44V4阳性率均低于低分化患者，且有淋巴结转移患者高于无转移患者（P＜0.05）。宫颈癌组织 LVD均高于鳞状细胞原位癌组织和正常组织，高中分化和有淋巴结转移的宫颈癌组织LVD较高（P＜0.05）。宫颈癌组织中整合素α2β1和CD44V4表达呈正相关（r=0.687，P＜0.05），整合素α2β1表达与LVD呈正相关（r=0.559，P＜0.05），CD44V4表达与LVD呈正相关（r=0.612，P＜0.05）。［结论］ 整合素α2β1和CD44V4可促进早期宫颈鳞癌的发生、发展和淋巴结转移，两者可能发挥协同作用。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the relationship between integrinα2β1 and CD44V4 and lymph node metastasis in early cervical squamous cell carcinoma. ［Methods］ A total of 81 samples of Ⅰb1 stage specimens of cervical squamous cell carcinoma，20 normal cervical tissues and 20 cervical squamous cell carcinoma in situ were selected in this study. The expression of integrin α2β1 and CD44V4 in the tissues was detected by real-time quantitative PCR and immunohistochemistry，and the lymphatic density(LVD) marked by D2-40 was detected by immunohistochemistry. Pearson analysis was used to examine the correlation between integrin alphaα2β1，CD44V4 and LVD. ［Results］ The expression of integrinα2β1 and CD44V4 mRNA in cervical cancer tissues(1.1±0.2 and 1.3±0.1) was higher than that in normal cervical tissue(0.1±0.0 and 0.1±0.0) and squamous cell carcinoma tissue(0.3±0.1 and 0.3±0.1)(P＜0.05). The positive rate of integrinα2β1 and CD44V4 protein in cervical cancer tissues(87.7% and 85.2%) was higher than that in normal tissue(5.0% and 10.0%) and squamous cell carcinoma tissue(70.0% and 40.0%)(P＜0.05). The positive rates of integrinα2β1 and CD44V4 in patients with differentiated cervical cancer were lower than those with low differentiation，and the patients with lymph node metastasis were higher than those without metastasis(P＜0.05). The LVD of cervical cancer tissue was higher than the squamous cell carcinoma in situ and normal tissue. High differentiation and lymph node metastasis of cervical cancer with higher LVD(P＜0.05). Pearson analysis showed that the expression of integrinα2β1 and CD44V4 in cervical cancer tissues was positively correlated(r=0.687，P＜0.05)，and the expression of integrinα2β1 was positively correlated with LVD(r=0.559，P＜0.05)，and the expression of CD44V4 was positively correlated with LVD(r=0.612，P＜0.05). ［Conclusion］ Integrinα2β1 and CD44V4 can promote the occurrence，development and lymph node metastasis of early cervical squamous cell carcinoma. They may play a synergistic role and need further study. |
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