| 郁汉旭,尹 丽,何 侠.同源形成素样蛋白2在食管癌中的表达及细胞生物学功能分析[J].肿瘤学杂志,2019,25(11):966-971. |
| 同源形成素样蛋白2在食管癌中的表达及细胞生物学功能分析 |
| Expression and Cellular Biological Function of Formin-like 2 in Esophageal Cancer |
| 投稿时间：2019-08-26 |
| DOI：10.11735/j.issn.1671-170X.2019.11.B008 |
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| 中文关键词: 食管肿瘤 FMNL2 预后 细胞生物学 |
| 英文关键词:esophageal neoplasm formin-like 2 prognosis cell biology |
| 基金项目:国家自然科学基金（81672989）；江苏省卫生与计划生育委员会青年基金（Q201501） |
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| 中文摘要: |
| 摘 要：［目的］ 探究食管癌中同源形成素样蛋白2(Formin-like 2，FMNL2 )的表达及其与患者预后的关系，分析其在细胞中的生物学功能。［方法］ 使用免疫组化分析食管癌患者中FMNL2的表达；Kaplan-Meier分析FMNL2的表达与临床预后的关系，Cox回归模型分析FMNL2在预后中的价值。使用qRT-PCR和Western blot实验检测细胞中FMNL2的表达；使用CCK8和Transwell小室实验分析FMNL2对细胞增殖、侵袭与迁移的影响。［结果］ FMNL2在食管癌组织中表达高于癌旁组织（124.395±18.422 vs 37.876±18.266，P<0.05）；FMNL2表达与TNM分期有关(χ2=6.411，P=0.011)；Kaplan-Meier分析显示，与FMNL2低表达组相比，高表达组总生存时间（OS）、无病生存时间（DFS）均明显缩短(47.615 vs 30.125，P=0.01；50.923 vs 41.292，P=0.001)；单因素和多因素分析发现FMNL2表达是患者OS的危险因素(HR=3.671，95%CI：1.272～10.591，P=0.016；HR=3.660，95%CI：1.211～11.065，P=0.021)；FMNL2在食管癌细胞株中表达高于正常食管上皮细胞；沉默FMNL2能够显著抑制食管癌细胞的增殖、侵袭与迁移能力（P<0.05）。［结论］ FMNL2在食管癌发生发展过程中具有重要作用，有望成为食管癌的临床治疗新靶标。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the expression，cellular biological functions，and prognostic value of formin-like 2(FMNL2) in esophageal cancer(EC). ［Methods］ The expression of FMNL2 in EC tissues and adjacent normal tissues was detected by using immunohistochemistry.The relationship between the expression of FMNL2 and prognosis was analyzed by using Kaplan-Meier plots. Univariate analysis and multivariate analysis were performed by using Cox proportional hazards models. The expression of FMNL2 in EC cells was detected by using quantitative real-time PCR and Western blotting. CCK8 analysis was performed to explore the effect of FMNL2 on EC cell viability，while transwell assays were adopted to investigate the effects of FMNL2 on the cell invasion and migration.［Results］ FMNL2 expression was significantly higher in EC tissues than in adjacent normal tissues(124.395±18.422 vs 37.876±18.266，P<0.05). FMNL2 expression was related to TNM stage(χ2=6.411，P=0.011). Kaplan-Meier analysis demonstrated that the overall survival(OS) and disease-free survival(DFS) were significantly shorter in the high FMNL2 expression group than in the low FMNL2 expression group(47.615 vs 30.125，P=0.01；50.923 vs 41.292，P=0.001). Univariate and multivariate analyses showed that the expression of FMNL2 was an independent risk factor for OS(HR=3.671，95%CI：1.272~10.591，P=0.016；HR=3.660，95%CI：1.211~11.065，P=0.021). FMNL2 expression was higher in EC cells than in normal esophageal epithelial cells. FMNL2 knockdown significantly suppressed the prolife-ration，invasion，and migration of ECcells(P<0.05). ［Conclusion] FMNL2 plays important roles in the development and progression of EC，serving as a potential therapeutic target against EC. |
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