| 郝美丽,陈士花,谭 叶.重组人血管内皮抑素对肿瘤血管正常化时间窗的研究[J].肿瘤学杂志,2019,25(11):962-965. |
| 重组人血管内皮抑素对肿瘤血管正常化时间窗的研究 |
| Time Window of Tumor Vascular Normalization by rh-Endostatin |
| 投稿时间：2019-07-18 |
| DOI：10.11735/j.issn.1671-170X.2019.11.B007 |
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| 中文关键词: 重组人血管内皮抑素 血管正常化 G蛋白调节信号5 微血管密度 |
| 英文关键词:endostar vascular normalization RGS5 microvessel density |
| 基金项目: |
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| 中文摘要: |
| 摘 要：［目的］ 探讨重组人血管内皮抑素对小鼠胃癌移植瘤的血管正常化时间窗及其可能机制。［方法］ 建立50只MFC小鼠胃癌移植瘤模型，随机分成对照组与恩度（rh-ES）组：对照组小鼠尾静脉注射生理盐水（0.2ml/d）；恩度组小鼠尾静脉注射rh-ES［20mg/（kg·d）］，分别于治疗后 d1、d3、d5、d7、d9各处死5只小鼠。取出肿瘤，观察并测量各组肿瘤体积变化，绘制肿瘤生长曲线，采用免疫组化方法检测各组肿瘤组织中MVD、Collagen-Ⅳ、RGS5和HIF-1α表达情况。［结果］ rh-ES组较对照组肿瘤生长慢。rh-ES组在d5～9，CD34、RGS5、HIF-1α表达降低，Collagen-Ⅳ表达升高，与对照组相比差异有统计学意义(P<0.05)。［结论］ 恩度作用于胃癌小鼠，在第5～9d肿瘤微血管产生一个短暂趋于正常化时间窗，该时间窗内肿瘤细胞乏氧改善，可能与MVD、Collagen-Ⅳ、RGS5、HIF-1α有关。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the normalization time window of recombinant human endostatin(rh-endostatin) in mouse gastric cancer xenografts and its possible mechanism. ［Methods］ Fifty MFC mice bearing gastric cancer xenograft were randomly divided into control(NS) group and Endo(rh-ES) group(n=25 in each group). Mice in NS group were injected with normal saline(0.2ml/d) via the tail vein，while mice in rh-ES group were intravenously administered with 20 mg/(kg·d) rh-endostatin；batches of 5 mice in each group were sacrificed at d1，d3，d5，d7，and d9 after treatment. Tumors were harvested，and the tumor volume changes of each group were observed and measured. The tumor growth curve was drawn and the tumor inhibition rate was calculated. The microvessel density(MVD)，the expression of collagen-Ⅳ，RGS5 and HIF-1α in each group were detected by immunohistochemistry.［Results］ Compared with the NS group，the rh-ES group had slower tumor growth and higher tumor inhibition rate. In the rh-ES group at d5～9，the MVD(CD34 expression) and RGS5 expression were decreased，the expression of collagen-Ⅳ increased and HIF-1α decreased significantly compared with NS group( all P<0.05). ［Conclusion］ When endostatin acts on gastric cancer mice，a transient normalization time window of tumor microvessels was produced at the 5th to 9th day. During this time window，the hypoxia of tumor cells is improved，in which the MVD，collagen-Ⅳ，RGS5 and HIF-1α. may be involved. |
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