| 姚若愚,展 浩,张旭光.辐射旁效应再传递的实验观察[J].肿瘤学杂志,2019,25(11):957-961. |
| 辐射旁效应再传递的实验观察 |
| Experimental Observation on Retransmission of Radiation Side Effects |
| 投稿时间：2019-05-19 |
| DOI：10.11735/j.issn.1671-170X.2019.11.B006 |
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| 中文关键词: 辐射旁效应 再传递 细胞 |
| 英文关键词:radiation side effect retransmission cell |
| 基金项目:江苏省自然科学基金项目(BK20151156) |
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| 中文摘要: |
| 摘 要：［目的］ 观察辐射旁效应在细胞间的再传递。［方法］ 用6MV-X射线照射鼠卵巢癌NUTU19细胞株，其培养液制备一代条件培养液，处理一代效应细胞鼠源NUTU19细胞株、肠黏膜上皮IEC-6细胞株，由一代鼠源效应细胞的分泌物为二代条件培养液，再处理二代效应细胞NUTU19细胞株、IEC-6细胞株和鼠淋巴细胞，检测细胞生长活力和凋亡水平。［结果］NUTU19二代培养液处理细胞48h后，对IEC-6和NUTU19细胞的凋亡率有促进提高作用（P<0.05），对淋巴细胞的凋亡率有降低作用（P＜0.05）。NUTU19二代培养液处理细胞72h后，促进NUTU19、IEC-6的凋亡（P＜0.05），对淋巴细胞凋亡率无影响（P>0.05）。IEC-6二代培养液处理细胞48h后，对NUTU19、IEC-6的凋亡有促进作用（P＜0.05），对淋巴细胞的凋亡有降低作用（P＜0.05）。IEC-6二代培养液处理细胞72h后，对IEC-6、淋巴细胞的凋亡均无影响（P＞0.05）。对NUTU19的凋亡有促进作用（P＜0.05）。［结论］ 在一定的条件下，肿瘤细胞、肠上皮细胞、淋巴细胞都能够再传递辐射旁效应的损伤。 |
| 英文摘要: |
| Abstract：［Objective］ To observe the retransmission of radiation side effects between cells. ［Methods］ The mouse ovarian cancer cell line NUTU19 was irradiated with 6MV-X-rays，and the culture medium was prepared for the first-generation conditioned medium. The first-generation effector cells were used to detect the NUTU19 cell line and the intestinal mucosal epithelial IEC-6 cell line. The secretion of effector cells was a second-generation conditioned medium，and the second-generation effector cells NUTU19，IEC-6 and mouse lymphocytes were treated to measure cell viability and apoptosis.［Results］ After treated with NUTU19 second-generation medium for 48 hours，the apoptosis rate of IEC-6 and NUTU19 cells was promoted(P<0.05)，and the apoptosis rate of lymphocytes was decreased(P<0.05). After treatment with NUTU19 second-generation medium for 72h，the apoptosis of NUTU19 and IEC-6 was promoted(P<0.05)，and there was no effect on the apoptosis rate of lymphocytes(P>0.05). After treatment with IEC-6 second-generation medium for 48h，the apoptosis of NUTU19 and IEC-6 was promoted(P<0.05)，and the apoptosis of lymphocytes was decreased(P<0.05). After treated with IEC-6 second-generation medium for 72h，there was no effect on IEC-6 and lymphocyte apoptosis(P>0.05). It promoted the apoptosis of NUTU19(P<0.05). ［Conclusion］ Under certain conditions，tumor cells，intestinal epithelial cells and lymphocytes can retransmit the damage of the side effect of radiation. |
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