| 陈 勇,姜 泳.SPON1表达对骨肉瘤细胞侵袭、转移能力的影响[J].肿瘤学杂志,2019,25(10):882-886. |
| SPON1表达对骨肉瘤细胞侵袭、转移能力的影响 |
| Effect of SPON1 Expression on Invasion and Metastasis of Osteosarcoma Cells |
| 投稿时间：2018-07-26 |
| DOI：10.11735/j.issn.1671-170X.2019.10.B006 |
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| 中文关键词: SPON1 骨肉瘤 迁移 侵袭 |
| 英文关键词:SPON1 osteosarcoma migration invasion |
| 基金项目: |
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| 中文摘要: |
| 摘 要：［目的］ 探讨SPON1表达对骨肉瘤细胞侵袭、转移能力的影响。［方法］ 骨肉瘤细胞株KHOS分为对照组、空白转染组和si-SPON1组，其中空白转染组转染siRNA，si-SPON1组转染siRNA-SPON1，采用MTT法检测各组细胞增殖，采用Tranwell细胞侵袭和细胞划痕实验检测细胞侵袭和迁移能力，Western blot检测SPON1蛋白以及FAK/Src信号通路蛋白表达。［结果］ si-SPON1组SPON1蛋白相对表达量为0.212±0.087，明显低于对照组和空白转染组（P＜0.05）；对照组、空白转染组和si-SPON1组转染12h、24h、48h和72h吸光度A值比较差异无统计学意义（P＞0.05）；si-SPON1组细胞迁移率和侵袭细胞数分别为14.08%±3.11%和75.64±24.20个，明显低于对照组和空白转染组（P＜0.05）；si-SPON1组p-FAK和p-Src相对表达量分别为0.354±0.100和0.310±0.101，均低于对照组和空白转染组（P＜0.05）；对照组、空白转染组和si-SPON1组FAK和Src蛋白相对表达量比较差异无统计学意义（P＞0.05）。［结论］抑制SPON1蛋白表达，可降低骨肉瘤KHOS细胞迁移和侵袭能力，其机制可能与FAK/Src信号通路有关。 |
| 英文摘要: |
| Abstract：［Objective］To investigate the effect of SPON1 expression on invasion and metastasis of osteosarcoma cells. ［Methods］ The osteosarcoma KHOS cells were divided into control group，blank transfection group and si-SPON1 group，the blank transfection group was transfected into siRNA，and the si-SPON1 group was transfected into siRNA-SPON1. The cell proliferation was detected by MTT，and invasion and migration of cells were detected by Tranwell and scratch test，the expression of SPON1 protein and FAK/Src signaling protein was detected by Western blot. ［Results］ The relative expression of SPON1 protein in si-SPON1 group was 0.212±0.087，which were significantly lower than that in control group and blank transfection group(P<0.05). There were no significant differences in the absorbance A value at 12h，24h，48h and 72h after transfection among three groups(P>0.05). The cell migration rate and the number of invasive cells in the si-SPON1 group were 14.08%±3.11% and 75.64±24.20，which were significantly lower than those in the control group and the blank transfection group(P<0.05). The relative expression of p-FAK and p-Src in si-SPON1 group were 0.354±0.100 and 0.310±0.101，which were significantly lower than those in control group and blank transfection group(P<0.05). There were no significant differences in the relative expression of FAK and Src among three groups(P>0.05). ［Conclusion］ Inhibition of SPON1 protein expression can inhibit the migration and invasion of osteosarcoma KHOS cells，which may be related to FAK/Src signaling pathway. |
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