| 余 斌,丁佑铭,廖晓锋.miR-621在肝细胞癌组织中的表达及其临床意义[J].肿瘤学杂志,2019,25(10):869-875. |
| miR-621在肝细胞癌组织中的表达及其临床意义 |
| Expression of miR-621 in Hepatocellular Carcinoma and Its Clinical Significance |
| 投稿时间：2018-06-22 |
| DOI：10.11735/j.issn.1671-170X.2019.10.B004 |
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| 中文关键词: 肝细胞癌 miR-621 预后 TCGA数据库 GEO数据库 |
| 英文关键词:hepatocellular carcinoma miR-621 prognosis TCGA database GEO database |
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| 中文摘要: |
| 摘 要：［目的］ 探讨肝细胞癌（hepatocellular carcinoma，HCC）组织中miR-621的表达及其临床意义，并对miR-621靶基因进行系统的生物信息学分析。［方法］ 基于GEO（Gene Expression Omnibus）和TCGA（The Cancer Genome Atlas）数据库，比较HCC组织和癌旁组织中miR-621表达量，并分析miR-621表达与HCC患者临床病理特征及预后的相关性。利用生物信息学方法对miR-621的靶基因进行预测及功能富集分析，并结合蛋白互作网络及预后分析结果筛选miR-621关键靶基因。［结果］ miR-621在HCC组织中较癌旁组织低表达（P<0.05）。HCC组织中miR-621表达量与患者TNM分期、肿瘤分化程度以及血清甲胎蛋白（AFP）等指标显著相关（P均<0.05）。生存分析显示HCC组织中miR-621低表达是影响患者预后的独立危险因素（P<0.05）。富集分析提示miR-621的靶基因主要富集于细胞黏附、氨基酸跨膜运输、钙离子结合等功能，以及MAPK、细胞周期等信号通路。AURKA、CDC25A、ESPL1、GINS2、KIF20B、MCM5、NUSAP1、OIP5等为miR-621关键靶基因，其在HCC组织中均呈现表达上调，且相对高表达者预后不良（P均<0.05）。［结论］ miR-621可作为一种抑癌基因参与HCC的发生、发展，并具有成为HCC诊断标志物、预后指标及治疗靶点的潜在价值。 |
| 英文摘要: |
| Abstract：［Objective］ To analyze the expression of miR-621 and its clinical significance in patients with hepatocellular carcinoma(HCC) using the data from GEO and TCGA. ［Methods］ The expression levels of miR-621 in HCC tissues and its adjacent liver tissues were compared with data from GEO and TCGA database，and the relations of miR-621 expression level with clinicopathologic characteristics and prognosis of the patients were also analyzed. The prediction of potential target genes of miR-621 and the functional enrichment analyses of the target genes were performed by bioinformatics analysis. Furthermore，the key target genes of miR-621were screened based on PPIs network and survival analysis. ［Results］ The expression levels of miR-621 were significantly downregulated in HCC tissues compared to the adjacent liver tissues(P<0.05). The expression level of miR-621 was positively correlated with TNM stage，pathological tumor grade and alpha-fetoprotein(all P<0.05). Survival analysis showed that low expression of miR-621 was associated with poor prognosis in patients with HCC(P＜0.05). Enrichment analyses revealed that the target genes of miR-621 were significantly associated with cell adhesion，calcium ion binding，amino acid transmembrane transporter activity，MAPK pathway and cell cycle signaling pathway. AURKA，CDC25A，ESPL1，GINS2，KIF20B，MCM5，NUSAP1 and OIP5 were the key target genes of miR-621，with significant upregulation in HCC tissues(all P＜0.05). Patients with high expression of these genes had relatively poor prognosis(all P＜0.05). ［Conclusion］ MiR 621 may play as an anti-oncogene miRNA in HCC，and it has a potential value of acting as a diagnostic biomarker，prognostic indicator and therapeutic target for HCC. |
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