| 肖艳景,薛金慧,白慧丽.miR-124-3p.1靶向PRDM2调节JAK/STAT通路对胰腺癌细胞增殖和凋亡的影响[J].肿瘤学杂志,2019,25(9):803-807. |
| miR-124-3p.1靶向PRDM2调节JAK/STAT通路对胰腺癌细胞增殖和凋亡的影响 |
| Effect of miR-124-3p.1 Targeting PRDM2 on Cell Proliferation and Apoptosis in PANC-1 via JAK/STAT Pathway |
| 投稿时间：2019-03-22 |
| DOI：10.11735/j.issn.1671-170X.2019.09.B008 |
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| 中文关键词: miR-124-3p.1 PRDM2 PANC-1 胰腺癌 增殖 凋亡 |
| 英文关键词: |
| 基金项目:河南省科技攻关计划项目（142102310461） |
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| 中文摘要: |
| 摘 要：［目的］ 研究miR-124-3p.1对胰腺癌细胞增殖、凋亡的影响，并探讨其作用机制。［方法］ 运用qRT-PCR法检测组织和细胞中miR-124-3p.1、PR结构域蛋白2(PRDM2)的表达；将miR-124-3p.1 mimics、miR-NC、anti-miR-124-3p.1 mimics、anti-miR-NC用脂质体法转染PANC-1细胞；Western blot检测细胞中PRDM2、p-JAK2、p-STAT3蛋白表达；MTT法检测细胞增殖。［结果］ 与人正常胰腺组织细胞相比，胰腺癌组织细胞中PRDM2表达显著性升高，miR-124-3p.1表达显著性降低（P<0.05）；PRDM2为miR-124-3p.1的靶标。过表达miR-124-3p.1可抑制胰腺癌细胞增殖、促进凋亡、抑制JAK/STAT通路。［结论］ miR-124-3p.1可抑制胰腺癌细胞增殖和促进凋亡，可能与靶向PRDM2和抑制JAK/STAT通路有关。 |
| 英文摘要: |
| Abstract：［Objective］ To study the effect of miR-124-3p.1 on cell proliferation and apoptosis of pancreatic cancer cells，and to explore its mechanism. ［Methods］ qRT-PCR was used to detect the expression of miR-124-3p.1 and PRDM2 in tissues and cells. miR-124-3p.1 mimics，miR-NC，anti-miR-124-3p.1 mimics，anti-miR-NC was transfected into PANC-1 cells by liposome method. The protein expression of PRDM2，p-JAK2 and p-STAT3 was detected by Western blot， cell proliferation was detected by MTT assay. ［Results］ Compared with normal pancreatic tissue and cells，the expression of PRDM2 was significantly increased，and the expression of miR-124-3p.1 was significantly decreased in pancreatic cancer tissues and cells(P<0.05). PRDM2 was a target of miR-124-3p.1. Overexpression of miR-124-3p.1 inhibited pancreatic cancer cell proliferation，promotes apoptosis，and inhibited JAK/STAT pathway. ［Conclusion］ miR-124-3p.1 could inhibit the proliferation and promote apoptosis of pancreatic cancer cells，which may be related to targeting PRDM2 and inhibiting JAK/STAT pathway. |
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