| 马 建,付旭东,周少龙.S100A6在胶质瘤组织中表达及对U87细胞增殖和侵袭力的影响[J].肿瘤学杂志,2019,25(9):798-802. |
| S100A6在胶质瘤组织中表达及对U87细胞增殖和侵袭力的影响 |
| Expression of S100A6 in Glioma Tissue and Its Effect on Proliferation and Invasion of U87 Cells |
| 投稿时间：2019-04-16 |
| DOI：10.11735/j.issn.1671-170X.2019.09.B007 |
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| 中文关键词: 胶质瘤 S100A6 小分子RNA干扰 迁移 侵袭 |
| 英文关键词:glioma S100A6 small interference RNA cell proliferation cell invasion |
| 基金项目:河南省自然科学基金（182300410379） |
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| 中文摘要: |
| 摘 要：［目的］ 探讨S100A6在胶质瘤组织中表达及对U87细胞增殖和侵袭力的影响。［方法］ 选取2017年3月至2019年3月在郑州大学第五附属医院择期行手术治疗的胶质瘤患者105例，利用实时荧光定量PCR技术检测胶质瘤和癌旁组织中S100A6基因表达，培养人胶质瘤U87细胞，根据转染物的不同将细胞分为siRNA-S100A6组、siRNA-对照序列组和空白对照组，利用实时荧光定量PCR技术和Western blot技术检测细胞中S100A6基因和蛋白表达，MTT法检测细胞增殖能力，划痕实验检测细胞迁移能力，Transwell小室检测细胞侵袭能力。［结果］ 胶质瘤组织中S100A6 mRNA相对表达量为1.95±0.16，癌旁组织中S100A6 mRNA相对表达量为1.33±0.13，两组差异有统计学意义（t=30.776，P<0.001）。与WHO分级Ⅰ～Ⅱ级相比，Ⅲ～Ⅳ级中S100A6mRNA表达量显著性升高（P<0.001）。与空白对照组和siRNA-对照序列组相比，siRNA-S100A6组细胞中S100A6 mRNA和蛋白表达量均降低（P均<0.001），24h、48h、72h和96h细胞增殖活性被抑制（P<0.05），划痕愈合率降低（P<0.001），且侵袭细胞数显著性减少（P<0.001）。［结论］ S100A6在胶质瘤组织中呈高表达，且与胶质瘤恶性程度有关，特异性沉默U87细胞中S100A6基因表达可有效抑制细胞增殖，降低细胞迁移和侵袭能力。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the expression of S100A6 in glioma tissue and its effect on the biological characteristics of U87 cells. ［Methods］ 105 cases of patients with glioma undergoing elective surgery treatment were selected from March 2017 to March 2019 in the Fifth Affiliated Hospital of Zhengzhou University. The expressions of S100A6 genes in gliomas tissues and adjacent tissues were detected by using real-time fluorescence quantitative PCR. The human glioma U87 cells were cultured. According to the different transfectants，the cells were divided into siRNA-S100A6 group，siRNA-control sequence group and blank control group. Real-time fluorescence quantitative PCR and Western blot were used to detect the expressions of S100A6 gene and protein. MTT assay was used to detect cell proliferation. Scaling experiment was performed to detect cell migration ability and Transwell chamber was used to detect cell invasion. ［Results］ The relative expression level of S100A6 mRNA in the glioma tissues was 1.95±0.16，while in the adjacent tissues was 1.33±0.13，the difference was statistically significant(t=30.776，P<0.001). Compared with WHO grade Ⅰ～Ⅱ，the expression level of S100A6 mRNA in grade Ⅲ～Ⅳ was significantly increased(P<0.001). Compared with the blank control group and the siRNA-control sequence group，the expression levels of S100A6 mRNA and protein in siRNA-S100A6 group were decreased(all P<0.001). Cell proliferation activitied at 24h，48h，72h and 96h were inhibited(P<0.05)，the wound healing rate was decreased(P<0.001)，and the number of invasive cells was significantly reduced(P<0.05). ［Conclusion］ S100A6 is highly expressed in glioma tissues，and was related to the degree of glioma malignancy. Specific silencing of S100A6 gene expression in U87 cells could effectively inhibit cell proliferation and reduce cell migration and invasion. |
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