| 唐 雷,周 莉,吴胜春.microRNA-10a联合microRNA-23a在结直肠腺癌早期诊断中的价值[J].肿瘤学杂志,2019,25(8):712-716. |
| microRNA-10a联合microRNA-23a在结直肠腺癌早期诊断中的价值 |
| Value of MicroRNA-10a and MicroRNA-23a in Diagnosis of Colorectal Adenocarcinoma |
| 投稿时间：2018-03-30 |
| DOI：10.11735/j.issn.1671-170X.2019.08.B007 |
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| 中文关键词: 结直肠腺癌 microRNA-10a microRNA-23a 早期诊断 |
| 英文关键词:colorectal adenocarcinoma microRNA-10a microRNA-23a early diagnosis |
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| 摘要点击次数: 1574 |
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| 中文摘要: |
| 摘 要：［目的］探讨血清microRNA-10a及microRNA-23a水平在结直肠腺癌早期诊断中的价值。［方法］ 选择经肠镜病理活检证实为结直肠腺癌患者30例作为研究组，另选取同期接受治疗的结直肠良性病变患者30例作为对照组，所有患者均抽取外周静脉血，采用实时荧光定量PCR技术（real-time PCR，qRT-PCR）检测并比较研究组患者术前、术后7d及对照组患者的血清miR-10a及miR-23a表达水平，通过受试者工作特性曲线（receiver operator characteristic curve，ROC）判断各单项miRNA表达水平及两者联合在结直肠腺癌诊断中的灵敏度和特异性。［结果］ （1）研究组患者术前血清miR-10a及miR-23a表达水平均显著升高，明显高于对照组（t=9.376、9.584，P均<0.001），术后则显著降低，与术前比较差异均有统计学意义（t=4.231、4.246，P均<0.001）。（2）ROC曲线分析结果显示，单用血清miR-10a检测结直肠腺癌的灵敏度为75.7%，特异性为86.2%；单用血清miR-23a检测结直肠腺癌的灵敏度为73.4%，特异性为87.3%；两者联合检测结直肠腺癌的灵敏度为89.8%，特异性为81.6%，且可产生最大ROC的曲线下面积（AUC）为0.894。［结论］ miR-10a及miR-23a生物标志物组可能是一种新型结直肠腺癌早期诊断的指标。 |
| 英文摘要: |
| Abstract：［Objective］ To assess the application of serum microRNA-10a(miR-10A) and microRNA-23a(miR-23A) levels in diagnosis of colorectal adenocarcinoma. ［Methods］ Thirty patients with colorectal adenocarcinoma confirmed by colonoscopic biopsy(study group) and 30 patients with colorectal benign lesions(control group) were enrolled in the study. Serum miR-10a and miR-23a levels were detected by Real-time quantitative RT-PCR(qRT-PCR) before and 7d after the operation，and the levels were compared between the study group and the control group. The sensitivity and specificity of serum miR-10a，miR-23a and the combination of two microRNAs in the diagnosis of colorectal adenocarcinoma were evaluated by receiver operator characteristic curve(ROC). ［Results］ The serum levels of miR-10a and miR-23a were significantly higher in the study group than those in the control group(t=9.376，9.584，all P<0.001)，and decreased significantly after the operation(t=4.231，4.246 all P<0.001). ROC curve analysis showed that the sensitivity of serum miR-10a and miR-23a in detection of colorectal adenocarcinoma was 75.7% and 73.4%；the specificity of two microRNAs was 86.2% and 87.3%，respectively. The sensitivity and specificity of the combination of miR-10a and miR-23a was 89.8% and 81.6%，respectively；and the area under the curve(AUC) of ROC curve was 0.894. ［Conclusion］ Circulating miR-10a and miR-23a may be used as biomarkers for diagnosis of colorectal adenocarcinoma. |
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