| 沈 磊,张平平,杨海涛.Keap1-Nrf2-ARE信号通路在胃腺癌细胞中的表达及意义[J].肿瘤学杂志,2019,25(8):703-706. |
| Keap1-Nrf2-ARE信号通路在胃腺癌细胞中的表达及意义 |
| Expression and Clinical Significance of Keap1-Nrf2-ARE Signaling Pathway in Gastric Adenocarcinoma |
| 投稿时间：2018-11-07 |
| DOI：10.11735/j.issn.1671-170X.2019.08.B005 |
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| 中文关键词: Keap1-Nrf2-ARE 胃腺癌 表达 |
| 英文关键词:Keap1-Nrf2-ARE gastric adenocarcinoma expression |
| 基金项目:安徽省自然科学基金青年项目（1808085QH277） |
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| 中文摘要: |
| 摘 要：［目的］ 检测Keap1-Nrf2-ARE信号通路相关蛋白在胃腺癌组织中的表达，探讨其作用和临床意义。［方法］ 选择64例胃腺癌组织，对应的癌旁组织为对照，应用免疫组织化学的方法检测癌组织及对照组织中细胞核因子E2相关因子2（Nrf2）以及抗氧化应答元件(ARE)的表达。［结果］ 胃癌组织中Nrf2、ARE的表达水平分别为1.78±0.34、1.69±0.45，均显著高于癌旁组织(0.47±0.16、0.52±0.22)，差异有统计学意义（P<0.001）。胃癌低/中分化患者中，Nrf2、 ARE的阳性表达率分别为79.1%、88.4%，均显著高于高分化患者（P<0.05）；TNM 分期Ⅲ、Ⅳ期患者Nrf2、ARE的阳性表达率分别为81.4%和 90.7%，均显著高于Ⅰ、Ⅱ期患者，差异有统计学意义（P<0.05）；有淋巴结转移患者Nrf2、ARE的阳性率分别为80.5%和 92.7%，显著高于无淋巴结转移的患者（P<0.05）。相关性分析显示Nrf2与ARE蛋白在胃腺癌组织中的表达呈显著正相关（χ2=17.322，P<0.001）。［结论］ 胃腺癌患者癌组织中Nrf2与ARE蛋白表达上调，Keap1-Nrf2-ARE信号通路与胃腺癌的发生发展密切相关。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the expression of Keap1-Nrf2-ARE signaling pathway-related protein in gastric adenocarcinoma and its clinical significance. ［Method］ The expression of nuclear factor E2-related factor 2(Nrf2) and anti-oxidant responsive element(ARE) in 64 samples of gastric adenocarcinoma and the corresponding adjacent tissues were detected with immunohistochemistry. ［Results］ The expression levels of Nrf2 and ARE in cancer tissues were 1.78±0.34 and 1.69±0.45，which were significantly higher than those in adjacent tissues(P<0.05). The positive rates of Nrf2 and ARE in poor and moderately differentiated tumors was 79.1% and 88.4% in cancer tissues，which were higher than those in well differentiated tumors(P<0.05). The positive rates of Nrf2 and ARE was 81.4% and 90.7% in TNM stage Ⅲ and Ⅳ patients，which were significantly higher than those in stage Ⅰ and Ⅱ gastric adenocarcinoma.(P<0.05). In patients with lymph node metastasis，the positive rates of Nrf2 and ARE was 80.5% and 92.7%，which were significantly higher than those of patients without lymph node metastasis(P<0.05). Correlation analysis showed a significant positive correlation between Nrf2 and ARE protein expression in gastric adenocarcinoma tissues(χ2=17.322，P<0.001). ［Conclusion］ The expression of Nrf2 and ARE protein are up-regulated in the cancer tissues of patients with gastric adenocarcinoma，indicating that Keap1-Nrf2-ARE signaling pathway may be closely related to the occurrence and development of gastric adenocarcinoma. |
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