| 屈重行,刘艳红,葛瑞瑞.胃癌组织中p53蛋白表达和微卫星不稳定及其与预后的相关性研究[J].肿瘤学杂志,2019,25(8):698-702. |
| 胃癌组织中p53蛋白表达和微卫星不稳定及其与预后的相关性研究 |
| P53 Protein Expression and Microsatellite Instability in Gastric Carcinoma and Their Relationship with Prognosis |
| 投稿时间：2018-10-15 |
| DOI：10.11735/j.issn.1671-170X.2019.08.B004 |
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| 中文关键词: 胃肿瘤 微卫星不稳定 p53 预后 |
| 英文关键词:gastric carcinoma microsatellite instability p53 prognosis |
| 基金项目:河南省科研立项 (162102310228) |
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| 中文摘要: |
| 摘 要：［目的］ 探讨胃癌组织中 p53 蛋白表达与微卫星不稳定(microsatellite instability，MSI)与胃癌临床病理特征及预后的关系。［方法］ 选取行胃癌手术患者104例，采用免疫组织化学法检测肿瘤组织中p53蛋白和错配修复蛋白(mismatch repair protein，MMRP)（MLH1、MSH2、MSH6和PMS2）表达，分析p53表达和MSI与患者临床病理参数的关系。术后患者随访2年，采用Logistics回归分析影响患者预后的危险因素。［结果］ 胃癌组织中p53、MLH1、MSH2、MSH6和PMS2均定位于肿瘤细胞核。104例胃癌中p53阳性46例(44.23%)；MMRP阳性65例(62.50%)，39例MMRP表达缺失(37.50%)，其中MLH1、MSH2、MSH62及PMS表达缺失分别为25例(24.04%)、19例(18.27%)、18例(17.31%)和 22例(21.15%)。胃癌低分化组及Ⅲ期组中p53阳性表达率显著降低(P<0.05)。p53在癌组织中的表达与MSI呈负相关(r=-0.486，P<0.05)。Logistic多因素回归分析显示，TNM分期、MSI和p53阴性是影响患者预后的独立危险因素。［结论］ 胃癌组织中p53与MSI呈负相关。对胃癌组织监测p53与MSI对制定给药方案及评估预后具有重要意义。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the relationship of p53 expression and microsatellite instability(MSI) with the clinicopathological features and prognosis of gastric cancer. ［Methods］ One hundred and four patients with gastric carcinoma undergoing gastrectomy were enrolled. Expression of p53 protein and mismatch repair protein(MMRP) MLH1，MSH2，MSH6 and PMS2 in gastric carcinoma tissues were detected by immunohistochemistry. The relationship between p53 expression and MSI with clinicopatholigical parameters were analyzed. All patients were followed up for 2 years，the risk factors affecting the prognosis of patients was analyzed by Logistic regression.［Results］ The expressions of P53，MLH1，MSH2，MSH6 and PMS2 were localized in nuclei in gastric carcinoma cells. Among 104 cases of gastric cancer，46 cases were p53 positive(44.23%)，65 cases(62.50%) were MMRP positive and 39 cases(37.50%) were MMRP negative. Among them，the missing of MLH1，MSH2，MSH6 and PMS2 was 25 cases(24.04%)，19 cases(18.27%)，18 cases(17.31%) and 22 cases(21.15%)，respectively. The expression of p53 was significantly lower in the poorly differentiated and stage Ⅲ gastric carcinoma(P<0.05). The expression of p53 was negatively correlated with MSI(r=-0.486，P<0.05). Logistic multivariate regression analysis showed that higher TNM staging，MSI and negative p53 were independent risk factors for poor prognosis in gastric carcinoma. ［Conclusion］ P53 is negatively correlated with MSI in gastric cancer tissues. The detection of p53 expression and MSI is of value for treatment monitoring and prognosis of gastric carcinoma patients. |
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