| 陈 梅,钟 颖,明建中.miRNA-145在卵巢癌组织中的表达及其对增殖、迁移与侵袭的影响[J].肿瘤学杂志,2019,25(7):607-611. |
| miRNA-145在卵巢癌组织中的表达及其对增殖、迁移与侵袭的影响 |
| Expression of miRNA-145 in Ovarian Cancer and Its Effect on Proliferation，Migration and Invasion |
| 投稿时间：2018-07-05 |
| DOI：10.11735/j.issn.1671-170X.2019.07.B004 |
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| 中文关键词: miRNA-145 卵巢癌 细胞增殖 细胞迁移 细胞侵袭 |
| 英文关键词:miRNA-145 ovarian cancer cell proliferation cell migration cell invasion |
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| 中文摘要: |
| 摘 要：［目的］ 检测不同卵巢组织中miRNA-145的表达，并探讨miRNA-145在卵巢细胞SKOV-3增殖、迁移与侵袭的作用。［方法］ 采用定量聚合酶链反应（qPCR）测定正常卵巢组织、卵巢癌旁组织与卵巢癌卵巢组织的miRNA-145表达水平，随机将SKOV-3细胞分为正常对照组、空白病毒组和病毒转染组，分别采用MTT法测定各组细胞的增殖情况，采用qPCR测定miRNA-145的表达水平，采用划痕实验测定迁移能力，采用Transwell小室法测定侵袭能力。［结果］ 卵巢癌组织miRNA-145表达水平为0.56±0.23，较卵巢癌旁组织和正常卵巢组织显著性降低（P<0.05）。卵巢癌组织miRNA-145表达水平与患者国际妇产科联合会分期和组织分化程度相关（P<0.05），但与年龄无关（P >0.05）。病毒转染组细胞的miRNA-145表达水平为4.63±0.47，较正常对照组和空白病毒对照组显著性增加（P<0.05）。病毒转染组细胞在培养24h、48h和72h时OD值分别为0.68±0.15、1.17±0.23和1.62±0.26，均较正常对照组和空白病毒对照组显著性降低（P<0.05）。病毒转染组细胞的划痕宽度为675±46μm，较正常对照组和空白病毒对照组显著性增大（P<0.05）。病毒转染组穿膜细胞数量为36±6个，较正常对照组和空白病毒对照组显著性降低（P<0.05）。［结论］ miRNA-145低表达可能与卵巢癌的发生发展有关，过表达miRNA-145能够抑制卵巢癌细胞株的增殖、迁移和侵袭。miRNA-145可以作为治疗卵巢癌的新作用靶点。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the expression of miRNA-145 in ovarian carcinoma and its effect on cell proliferation，migration and invasion. ［Methods］ Expression of miRNA-145 in normal ovarian tissue，the tissue adjacent to ovarian cancer and ovarian cancer was determined by qRT-PCR. Human ovarian cancer SKOV-3 cells were transfected with miRNA-145-mimic(miRNA-145 group) or blank viral vector(miRNA-145-NC group)，and the untransfected SKOV-3 cells served as control group. Cell proliferation was determined by MTT method，expression of miRNA-145 were determined by qRT-PCR，cell migration was determined by scratch experiment，and cell invasion was determined by Transwell small chamber method.［Results］ The expression level of miRNA-145 in ovarian cancer tissue was 0.56±0.23，which was significantly lower than that in pericancerous ovarian tissue and normal ovarian tissue(P<0.05). The miRNA-145 expression level in ovarian cancer tissue was correlated with FIGO stages and differentiation degrees(P<0.05)，but not correlated with the age of patients(P>0.05). Expression level of miRNA- 145 in miRNA-145 group was 4.63±0.47，which was significantly higher than that in normal control group and miRNA-145-NC group(P<0.05). OD values in miRNA-145 group after incubation for 24h，48h and 72h were 0.68±0.15，1.17±0.23 and 1.62±0.26，which were significantly lower than those in normal control group and miRNA-145-NC group after incubation for 24h，48h and 72h(P<0.05). Scratch width of cells in miRNA-145 group was 675±46μm，which was significantly larger than that in normal control group and miRNA-145-NC group(P<0.05). Quantity of transfected cells in miRNA-145 group was 36±6，which was significantly lower than that in normal control group and miRNA-145-NC group(P<0.05). ［Conclusion］ Decreased expression of miRNA-145 may be associated with occurrence and development of ovarian cancer，over-expression of miRNA-145 can inhibit the proliferation，migration and invasion of ovarian cancer cells. miRNA-145 might be used as a new target for treatment of ovarian cancer. |
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