| 陈锦霞,单保恩,李清靖.IL-17及其相关分子在胃癌组织中的表达和相关性分析[J].肿瘤学杂志,2019,25(6):537-540. |
| IL-17及其相关分子在胃癌组织中的表达和相关性分析 |
| Expression of IL-17 and Related Chemokines in Gastric Cancer Tissues and Their Relation to Cancer Progression |
| 投稿时间：2018-06-20 |
| DOI：10.11735/j.issn.1671-170X.2019.06.B009 |
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| 中文关键词: 胃肿瘤 Th17细胞 IL-17 CCLx |
| 英文关键词:gastric cancer Th17 cells IL-17 CCLx |
| 基金项目:河北省自然科学基金（H2016206209）；河北省人才工程培养经费项目（A201401251）；河北省政府资助临床医学优秀人才项目 |
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| 中文摘要: |
| 摘 要：［目的］ 检测IL-17与趋化因子在胃癌组织中的表达，探讨IL-17在胃癌组织中聚集及其促胃癌进展的作用机制。［方法］收集35例手术切除胃癌组织标本，包括癌组织和非癌对照组织，采用荧光定量RT-PCR法检测IL-17及趋化因子CCL2、CCL7、CCL17、CCL20、CCL22、CXCL2和CXCL5 mRNA的表达；分析IL-17与表达增高的趋化因子的相关性；采用免疫组织化学染色法，检测胃癌组织内CD34和CD66b的表达。［结果］ 与非癌对照组织相比，胃癌组织中IL-17和趋化因子CCL2、CCL7、CCL20、CCL22 mRNA表达显著增高（P<0.05），而趋化因子CCL17、CXCL2和CXCL5 mRNA表达在两组间无明显差异（P>0.05）。胃癌组织中IL-17 mRNA表达水平升高与趋化因子CCL20、CCL22 mRNA表达水平升高明显相关（r=0.7901，P=0.0038；r=0.9683，P<0.0001），而与趋化因子CCL2、CCL7 mRNA表达水平升高无明显相关性（P>0.05）。IL-17 mRNA高表达组胃癌组织内CD34和CD66b的表达水平明显高于IL-17 mRNA低表达组（P<0.05）。［结论］ 胃癌组织内IL-17表达增高可能与胃癌组织高表达细胞因子CCL20、CCL22，趋化Th17细胞由外周循环向肿瘤募集有关，增高的IL-17通过促进新生血管生成和中性粒细胞浸润促进肿瘤进展。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the expression of IL-17 and related chemokines in gastric cancer tissues and their relation to cancer progression. ［Methods］ Tissue samples of gastric cancer and normal gastric mucosa were collected from 30 patients with gastric cancer. The mRNA expression of IL-17，and chemokines CCL2，CCL7，CCL17，CCL20，CCL22，CXCL2，CXCL5 were detected by qRT-PCR. The correlation between expression IL-17 and chemokines was determined by liner regression analysis. The expression of CD34 and CD66b in gastric cancer tissues were examined by immunohistochemical staining. ［Results］ Compared to normal mucosa，the mRNA expressions of IL-17，CCL2，CCL7，CCL20 and CCL22 in cancer tissues were significantly up-regulated(P<0.05)，while no difference was observed in mRNA expression of CCL17，CXCL2 and CXCL5(P>0.05). The high expression of IL-17 mRNA in tumor tissues were positively correlated with the expression of CCL20(r=0.7901，P=0.0038)and CCL22(r=0.9683，P<0.001)，but not correlated with CCL2 and CCL7(P>0.05). Furthermore，the expressions of CD34 and CD66b in gastric cancer with high expression of IL-17 mRNA were significantly higher than those with low expression of IL-17mRNA.［Conclusion］ The up-regulated expression of IL-17 mRNA in tumor tissues is associated with overexpression of CCL20 and CCL22 mRNA，indicating that IL-17 may promote tumor progression by inducing neovascularization and neutrophil infiltration. |
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