| 刘 晶,白 丽,李 宏.STAT3与miR-21的调控关系及其对舌鳞癌化疗敏感性的影响[J].肿瘤学杂志,2019,25(6):531-536. |
| STAT3与miR-21的调控关系及其对舌鳞癌化疗敏感性的影响 |
| Relationship of STAT3 and miR-21 Expression with Sensitivity to Cisplatin in Tongue Squamous Cell Carcinoma |
| 投稿时间：2018-03-05 |
| DOI：10.11735/j.issn.1671-170X.2019.06.B008 |
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| 中文关键词: miR-21 舌鳞癌 DDP 敏感性 STAT3 |
| 英文关键词:miR-21 tongue squamous cell carcinoma cisplatin sensitivity STAT3 |
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| 中文摘要: |
| 摘 要：［目的］ 探讨STAT3与miR-21的调控关系以及其对舌鳞癌细胞Tscca对顺铂（DDP）敏感性的影响作用。［方法］ 体外培养Tscca细胞和Tscca/DDPR细胞，将细胞分为空白对照组、阴性对照组、WP1066处理组、DDP处理组和WP1066+DDP共处理组。采用MTT法和划痕实验检测DDP和/或WP1066对Tscca细胞和Tscca/DDPR细胞增殖和迁移的影响；采用RT-PCR法和Western blot法检测不同处理方式Tscca细胞和Tscca/DDPR细胞miR-21、STAT3、p-STAT3的表达水平。［结果］ 经不同浓度DDP（0.25、1、4、16μg/ml）处理后，Tscca细胞的增殖和迁移能力明显低于Tscca/DDPR细胞（P<0.05），且具有剂量依赖性。Tscca细胞中miR-21、STAT3表达量明显低于Tscca/DDPR细胞（P<0.05）。WP1066可以明显抑制Tscca细胞和Tscca/DDPR细胞的增殖和迁移活性，且两组细胞比较差异无统计学意义（P>0.05）。经WP1066处理后，Tscca细胞、Tscca/DDPR细胞miR-21和p-STAT3表达水平均低于空白对照组（P＜0.05）。而DDP对Tscca/DDPR细胞miR-21和p-STAT3表达水平无明显影响，与空白对照组比较差异无统计学意义（P>0.05）。Pearson相关性分析显示，miR-21与p-STAT3的表达量呈正相关（r=0.999，P<0.05）。［结论］ STAT3/miR-21过表达与舌鳞癌细胞对顺铂的耐药性相关，有可能成为舌鳞癌患者对化疗药物产生耐药性的潜在干预靶点。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the relationship of signal transducer and activators of transcription 3(STAT3) and miR-21 expression and the sensitivity to cisplatin(DDP) in tongue squamous cell carcinoma. ［Methods］ Tongue squamous cell carcinoma Tscca cells and DDP-resistance Tscca cells(Tscca/DDPR) were cultured in vitro and divided into control group，negative control group，WP1066 treatment group，DDP treatment group and WP1066+DDP treatment group. The proliferation and metastasis ability of Tscca cells and Tscca/DDPR cells were detected by MTT and scratch healing assay，respectively. The mRNA and protein expressions of miR-21，p-STAT3，STAT3 in Tscca cells and Tscca/DDPR were detected by RT-PCR and Western blot，respectively. ［Results］ The proliferation and metastasis ability of Tscca cells treated with DDP(0.25，1，4，16μg/ml) were lower than those of Tscca/DDPR cells(P<0.05) in a dose-independent manner. The miR-21，STAT3 expressions in Tscca cells were lower than those in Tscca/DDPR cells(P<0.05). There was no difference in the proliferation and metastasis between Tscca and Tscca/DDPR cells treated with WP1066(P>0.05). And the miR-21 and p-STAT3 expressions in Tscca and Tscca/DDPR cells treated with WP1066 were lower than those in control group and negative control group(P<0.05). There was no difference in miR-21 and p-STAT3 expressions in Tscca/DDPR cells treated with DDP compared with control group(P>0.05). The miR-21 expression was positively correlated with p-STAT3(r=0.999，P<0.05). ［Conclusion］ The down-regulation of miR-21/STAT3 may enhance the sensitivity of tongue squamous cell carcinoma to DDP. |
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