| 张启弘,黄煜庆,兰霞斌.miR-155-5p通过调控hMLH1促进甲状腺乳头状癌增殖侵袭的研究[J].肿瘤学杂志,2019,25(6):492-497. |
| miR-155-5p通过调控hMLH1促进甲状腺乳头状癌增殖侵袭的研究 |
| MiR-155-5p Promotes Proliferation and Invasion of Papillary Thyroid Carcinoma through Regulation of hMLH1 Expression |
| 投稿时间：2018-11-16 |
| DOI：10.11735/j.issn.1671-170X.2019.06.B002 |
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| 中文关键词: 甲状腺乳头状癌 miR-155-5p hMLH1 |
| 英文关键词:papillary thyroid carcinoma miR-155-5p hMLH1 |
| 基金项目:浙江省医药卫生研究项目(2016KYA054)；国家自然科学基金(81702645) |
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| 中文摘要: |
| 摘 要：［目的］ 探讨microRNA-155-5p（miR-155-5p）在甲状腺乳头状癌（papillary thyroid carcinoma，PTC）中的表达及作用，验证其对靶基因错配修复酶人类mutL同系物1（human mutL homologue 1，hMLH1）的调控作用。［方法］采用实时定量PCR法检测miR-155-5p在PTC组织和细胞中的表达水平。采用MTT增殖实验、Transwell侵袭实验观察miR-155-5p对PTC细胞增殖和侵袭能力的影响。采用蛋白免疫印迹技术（Western blot）和荧光素酶报告实验验证miR-155-5p对hMLH1的表达调控。［结果］与癌旁正常组织相比，miR-155-5p在PTC组织中的表达水平明显增加，差异有统计学意义（t=3.492，P=0.002）；转染miR-155-5p mimics的PTC细胞中miR-155-5p的表达水平相比对照组明显增加，差异有统计学意义（TPC-1细胞株：t=4.214，P=0.002，BCPAP细胞株：t=4.268，P=0.002）；转染miR-155-5p mimics的PTC细胞相比对照组增殖能力明显增强，差异有统计学意义（TPC-1细胞株：48h t=4.378，P=0.012；BCPAP细胞株：48h t=22.106，P<0.001）；转染miR-155-5p mimics组通过Matrigel基质胶的细胞数量明显多于对照组，差异具有统计学意义（TPC-1细胞株：t=3.182，P=0.013；BCPAP细胞株：t=7.872，P<0.001）；转染miR-155-5p mimics的PTC细胞中hMLH1蛋白表达明显低于对照组，差异有统计学意义（TPC-1细胞株：t=5.137，P=0.007；BCPAP细胞株：t=3.684，P=0.021）。［结论］ miR-155-5p促进PTC增殖侵袭，其机制可能是通过调控hMLH1实现，这为PTC的治疗提供新的靶点。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effect of microRNA-155-5p(miR-155-5p) on proliferation and invasion of papillary thyroid carcinoma(PTC) and its molecular mechanism(hMLH1).［Method］ Real-time quantitative PCR(qRT-PCR) was used to detect the expression level of miR-155-5p in 20 paired specimens of PTC tissue and its adjacent tissue. The effects of miR-155-5p on the proliferation and invasion of PTC cells were observed by MTT assay and Transwell invasion assay. Western Blot and luciferase reporter assay were used to detect the expression of hMLH1(mismatch repair enzyme human mutL homologue 1). ［Results］ Compared with adjacent normal tissues，the expression of miR-155-5p was significantly increased in PTC tissues(t=3.492，P=0.002). The expression level of miR-155-5p in PTC TPC-1 cells and BCPAP cells transfected with miR-155-5p mimics was significantly higher than that of the control group(t=4.214，P=0.002，and t=4.268，P=0.002) .The proliferation ability of PTC TPC-1 cells and BCPAP cells transfected with miR-155-5p mimics was significantly higher than that of the control group(t=4.378，P=0.012 and t=22.106，P<0.001). The number of cells transfected with miR-155-5p mimics crossing through Matrigel was significantly higher than that of the control group(t=3.182，P=0.013 and t=7.872，P<0.001). The expression of hMLH1 protein in PTC cells transfected with miR-155-5p mimics was significantly lower than that in the control group(t=5.137，P=0.007 and t=3.684，P=0.021). ［Conclusion］ miR-155-5p promotes proliferation and invasion of PTC，which may be through the regulation of hMLH1 expression. |
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