| 刘 畅,张 珉,田 兴.MUC16对胆囊癌细胞生物学行为的调控作用及机制分析[J].肿瘤学杂志,2019,25(5):436-441. |
| MUC16对胆囊癌细胞生物学行为的调控作用及机制分析 |
| Effect of MUC16 on Biological Behavior of Gallbladder Carcinoma Cells and Its Mechanism |
| 投稿时间：2018-01-03 |
| DOI：10.11735/j.issn.1671-170X.2019.05.B012 |
|
 |
| 中文关键词: 黏蛋白16 胆囊肿瘤 增殖 侵袭 PI3K/Akt信号通路 |
| 英文关键词:mucin16 gallbladder proliferation invasion PI3K/Akt pathway |
| 基金项目:国家自然科学基金（U1404820） |
|
| 摘要点击次数: 2119 |
| 全文下载次数: 456 |
| 中文摘要: |
| 摘 要：［目的］ 探讨黏蛋白16（MUC16）对人胆囊癌细胞NOZ增殖、侵袭、转移的影响以及可能的分子机制。［方法］ 通过慢病毒转染体系，构建过表达MUC16的人胆囊癌细胞株NOZ，以及空病毒质粒转染细胞。MTT实验检测过表达MUC16对细胞体外增殖能力的影响；划痕实验和Transwell迁移小室实验检测过表达MUC16对细胞体外迁移和侵袭能力的影响；黏附实验检测过表达MUC16对细胞黏附能力的影响；Western blot检测MUC16调控NOZ细胞生物学行为可能的通路机制。［结果］ MUC16过表达细胞系生长速度明显高于对照组（P＜0.05）；并且MUC16转染NOZ细胞系体外迁移能力、划痕愈合能力以及黏附能力均较空质粒对照组细胞增强（P＜0.05）；MUC16可明显提高MMP2、MMP7、p-Akt蛋白的表达以及PI3K激酶的活性（P＜0.05）。［结论］ MUC16可以通过激活PI3K/Akt信号通路，增加胆囊癌细胞体外的增殖、侵袭以及转移能力。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effect and mechanism of mucin16(MUC16) on the invasion and metastasis ability of gallbladder carcinoma cells. ［Methods］ MUC16 lentivirus vector was constructed and transfected into gallbladder carcinoma NOZ cells. The cell proliferation was measured by MTT method，cell invasion ability was determined by Transwell membrane assay，cell metastasis ability was detected by wound scratch assay，and cell adhesion was tested by fibronection assay. The expression levels of MMP2 and MMP9 in NOZ cells were detected by Western blot. ［Results］ The proliferation，and invasion，metastasis and adhesion abilities in vitro of MUC16-transfected NOZ cells were increased compared to control cells(P<0.05). The expressions of MMP2 and MMP7 protein and p-Akt protein in NOZ-MUC16 cells were higher than those in the NOZ control cells(P<0.05). ［Conclusion］ MUC16 can increase the proliferation，and invasion，metastasis and adhesion abilities of gallbladder carcinoma cells in vitro by activating PI3K/Akt pathway. |
|
在线阅读
查看全文 查看/发表评论 下载PDF阅读器 |