| 王 磊,毕蓉蓉,尹 航.ENO1在胰腺上皮内瘤变和胰腺导管腺癌中的表达[J].肿瘤学杂志,2019,25(4):335-339. |
| ENO1在胰腺上皮内瘤变和胰腺导管腺癌中的表达 |
| Expression of ENO1 in Pancreatic Intraepithelial Neoplasia and Pancreatic Ductal Adenocarcinoma |
| 投稿时间：2018-01-28 |
| DOI：10.11735/j.issn.1671-170X.2019.04.B010 |
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| 中文关键词: 胰腺上皮内瘤变 胰腺导管腺癌 烯醇化酶-1 7，12-二甲基苯并蒽 |
| 英文关键词:pancreatic intraepithelial neoplasia pancreatic ductal adenocarcinoma enolase 1 7,12-dimethylbenzanthracene |
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| 中文摘要: |
| 摘 要：［目的］ 检测烯醇化酶-1（ENO1）在人和大鼠胰腺上皮内瘤变(PanIN)和胰腺导管腺癌组织中的表达情况。［方法］ 收集病理科存档人组织标本，胰腺导管腺癌18例，PanIN 4例，正常胰腺组织7例，免疫组化检测ENO1蛋白表达。SD大鼠20只采用胰腺包埋种植7，12-二甲基苯并蒽（DMBA）诱导法，建立大鼠PanIN和胰腺导管腺癌模型，Real-time PCR检测ENO1 mRNA表达，免疫组化和免疫印迹检测ENO1 蛋白表达。［结果］ ENO1蛋白在人PanIN（9.63±1.80）和胰腺导管腺癌组织（12.14±2.71）中的表达均显著高于人正常胰腺组织（P<0.01）。与造模后大鼠正常胰腺比较，ENO1 mRNA在大鼠PanIN-3（P<0.01）和胰腺导管腺癌组织（P<0.001）中表达显著增加，在大鼠PanIN-2（P<0.001）、PanIN-3（P<0.01）和PDAC（P<0.001）组织中，ENO1 蛋白表达呈进行性升高。［结论］ ENO1在PanIN和胰腺导管腺癌的表达显著增高，在胰腺上皮的癌变过程中可能发挥重要作用。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the expression of enolase 1(ENO1) in pancreatic intraepithelial neoplasia(PanIN) and pancreatic ductal adenocarcinoma(PDAC) tissues from human and rats.［Methods］ Eighteen tissue samples of PDAC，4 samples of PanIN and 7 samples of human normal pancreas tissue were collected，the expression of ENO1 protein was detected by immunohistochemistry. The rat model of PanIN and PDAC was induced by implantation with 7，12-dimethylbenzanthracene(DMBA) into pancreas in 20 adult male SD rats. The expression levels of ENO1 mRNA and protein in rat PanIN and PDAC tissues were detected by real-time PCR，immunohistochemistry and Western-blot，respectively. ［Results］ The expression of ENO1 protein in human PanIN(P<0.01) and PDAC(P<0.001) tissues was significantly higher than that in normal human pancreatic tissues. The expression of ENO1 mRNA was significantly increased in rat DMBA-induced PanIN-3(P<0.01) and PDAC(P<0.001)，and the expression of ENO1 protein was also significantly increased in rats PanIN-2(P<0.001)，PanIN-3(P<0.01) and PDAC tissues(P<0.001) compared to normal rat pancreas.［Conclusion］ENO1 expression is upregulated in PanIN and PDAC，indicating that ENO1 may be involved in the process of pancreatic epithelial carcinogenesis. |
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