| 刘 玉,王子安.食管鳞癌中CD151、ERK1/2及血管生成拟态的表达及临床意义[J].肿瘤学杂志,2019,25(4):330-334. |
| 食管鳞癌中CD151、ERK1/2及血管生成拟态的表达及临床意义 |
| Expression of CD151 and ERK1/2 in Esophageal Squamous Cell Carcinoma and Their Correlation with Vasculogenic Mimicry |
| 投稿时间：2018-06-22 |
| DOI：10.11735/j.issn.1671-170X.2019.04.B009 |
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| 中文关键词: 食管鳞状细胞癌 组织分化抗原151 细胞外调节蛋白激酶1/2 血管生成拟态 预后 |
| 英文关键词:ESCC CD151 ERK1/2 VM prognosis |
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| 中文摘要: |
| 摘 要：［目的］ 研究组织分化抗原151（CD151）及细胞外调节蛋白激酶1/2（ERK1/2）在食管鳞状细胞癌（ESCC）中的表达情况，分析两者表达与血管生成拟态（VM）的相关性。［方法］ 选取100例存档石蜡包埋ESCC组织标本作为观察组和50例癌旁（≥5cm）正常食管组织作为对照组，采用免疫组织化学ElivisionTM plus法检测ESCC中CD151及ERK1/2的表达，运用CD34/PAS双染检测食管癌中VM存在情况；并用Kaplan-Meier法分析VM与预后的关系。［结果］ 观察组CD151（82%）、ERK1/2（75%）蛋白阳性表达率及VM存在率（29%）均明显高于对照组（10%、36%、8%）（P均＜0.05）。CD151蛋白表达与ESCC的浸润深度、淋巴结转移、临床分期、分化程度有关（P均＜0.05）；ERK1/2蛋白表达与ESCC 的淋巴结转移、临床分期、分化程度有关（P均＜0.05）；ERK1/2在CD151阳性组的表达明显高于CD151阴性组，且两者表达呈正相关（r=0.451，P<0.001）；CD151和ERK1/2表达均与VM存在呈正相关（r=0.299，P=0.002；r=0.318，P=0.001）。Kaplan-Meier生存分析显示有VM组与无VM组5年生存率分别为10.3%和50.7%（P＜0.05）。Cox多因素回归分析显示：浸润深度、临床分期、VM存在是食管鳞癌患者术后5年生存率的独立影响因素（P均＜0.05）。［结论］ CD151、ERK1/2在食管鳞癌的发生及发展中具有重要作用，可能通过VM的形成，影响食管鳞癌的发生发展、转移及预后。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the expression of tissue differentiation antigen 151(CD151) and extracellular regulated protein kinase1/2(ERK1/2) in esophageal squamous cell carcinoma(ESCC)，and to analyze the their correlation with vasculogenic mimicry(VM). ［Methods］ One hundred samples of paraffin-embedded ESCC tissue and 50 samples of normal esophageal tissues were collected. The expression of CD151 and ERK1/2 in ESCC was detected by ElivisionTM plus method. The VM in esophageal cancer was detected by CD34/PAS double staining. The relationship between VM and prognosis was analyzed by Kaplan-Meier method. ［Results］ The positive rate of CD151(82%)，ERK1/2(75%) and the present rate of VM(29%) in the ESCC group were significantly higher than those in the control group(10%，36%，8%；all P<0.05). The expression of CD151 protein was correlated with the depth of invasion，lymph node metastasis，clinical stage and degree of differentiation of ESCC(all P<0.05). The expression of ERK1/2 protein was correlated with lymph node metastasis，clinical stage and differentiation of ESCC(all P<0.05). The expression of ERK1/2 was positively correlated with the expression of CD151 in ESCC(r=0.451，P<0.001)；the expression of CD151 and ERK1/2 was positively correlated with VM(r=0.299，P=0.002；r=0.318，P=0.001). Kaplan-Meier survival curve showed that the 5-years survival rate of VM group and non-VM group was 10.3% and 50.7%，respectively(P<0.05). Cox multivariate regression analysis demonstrated that the depth of invasion，clinical stage and VM were the independent factors of the 5-years survival(P<0.05). ［Conclusion］ The expression of CD151 and ERK1/2 are upregulated in ESCC，which may affect the development，metastasis and prognosis of ESCC through VM formation. |
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