| 范亚峰,崔晓燕,虞中平.Gab2基因在肺癌细胞表达及对癌细胞凋亡及Wnt/β-catenin信号通路的影响[J].肿瘤学杂志,2019,25(3):213-217. |
| Gab2基因在肺癌细胞表达及对癌细胞凋亡及Wnt/β-catenin信号通路的影响 |
| Expression of Gab2 Binding Protein 2 in Lung Cancer Cells and Its Relation to Apoptosis and Wnt/β-catenin Signaling Pathway |
| 投稿时间：2018-01-30 |
| DOI：10.11735/j.issn.1671-170X.2019.03.B008 |
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| 中文关键词: Gab2基因 肺癌 凋亡 Wnt/β-catenin信号通路 |
| 英文关键词:Gab2 gene lung cancer apoptosis Wnt/β-catenin signaling pathway |
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| 摘要点击次数: 1900 |
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| 中文摘要: |
| 摘 要：［目的］ 探讨Gab2结合蛋白2（Gab2）基因在肺癌细胞表达及对癌细胞凋亡及Wnt信号/β-连环蛋白（β-catenin）信号通路的影响。［方法］ 以人胚肺成纤维细胞MRC5作为对照细胞，通过Western bloting检测肺癌H322、A549、PC9、H1299、SPC-A-1细胞中Gab2的蛋白表达；转染48h后Western bloting检测Gab2、Cleaved Caspase-3、B细胞淋巴瘤/白血病-2（Bcl-2）、Bcl-2相关X蛋白(Bax)、β-连环蛋白(β-catenin)、细胞周期素D1（cyclin D1）、c-myc蛋白表达；流式细胞术检测各组细胞的凋亡情况。［结果］ 与MRC5细胞比较，Gab2在肺癌细胞中的表达显著性升高（P<0.05）。与对照组比较，Gab2-siRNA组Gab2 的蛋白表达降低，细胞凋亡率升高，Cleaved caspase3和Bax蛋白上调表达，Bcl-2、β-catenin、cyclin D1、c-myc蛋白表下调表达，差异均具有统计学意义（P<0.05）。［结论］ 抑制肺癌细胞Gab2表达能诱导细胞凋亡，机制是下调Wnt/β-catenin信号通路。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the expression of Gab2 gene in lung cancer cells and its relation to apoptosis and Wnt/β-catenin signaling pathway. ［Methods］ The expression of Gab2 protein in lung cancer H322，A549，PC9，H1299，SPC-A-1 cells and human embryonic lung fibroblast MRC5 cells was detected by Western blotting. The expression of Gab2，cleaved caspase3，Bcl-2，Bax，β-catenin，cyclin D1，c-myc protein were detected by Western blotting cells，cell apoptosis was detected by flow cytometry 48h after cells transfected with Gab2-siRNA. ［Results］ Compared with MRC5 cells，the expression of Gab2 in lung cancer cells increased significantly (P<0.05). Compared with the control group，the expression of Gab2 protein in Gab2-siRNA group was decreased，apoptosis rate increased，the expressions of cleaved caspase3 and Bax protein were up-regulated，and the expression of Bcl-2，β-catenin，cyclin D1 and c-myc protein was down-regulated (all P<0.05). ［Conclusion］ The expression of Gab2 in lung cancer cells is increased，inhibition of Gab2 expression induces apoptosis of lung cancer cells，which is associated with the down-regulated Wnt/β-catenin signaling pathway. |
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