| 高 大,吕爱娥,苏伊拉.MiR-410对多发性骨髓瘤细胞增殖和侵袭能力的影响[J].肿瘤学杂志,2019,25(2):133-139. |
| MiR-410对多发性骨髓瘤细胞增殖和侵袭能力的影响 |
| Effect of MiR-410 on Cell Proliferation and Invasion in Multiple Myeloma |
| 投稿时间：2017-09-06 |
| DOI：10.11735/j.issn.1671-170X.2019.02.B011 |
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| 中文关键词: 多发性骨髓瘤 miR-410 HMGB1 细胞增殖 细胞侵袭 |
| 英文关键词:multiple myeloma miR-410 HMGB1 cell proliferation cell invasion |
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| 中文摘要: |
| 摘 要：［目的］ 检测miR-410与HMGB1在多发性骨髓瘤中的表达水平及其对细胞增殖与侵袭能力的影响，探讨miR-410在多发性骨髓瘤致病中的作用。［方法］ 实时荧光定量PCR检测多发性骨髓瘤骨髓及细胞中miR-410的表达水平；蛋白质印迹用于分析HMGB1蛋白表达水平；CCK8实验和Boyden 侵袭小室实验分别用于检测细胞增殖活性及侵袭能力；双荧光素酶报告实验用于检测miR-410与HMGB1之间的相互作用；通过重组质粒构建及细胞转染调节相关基因在细胞内的表达；通过建立裸鼠异位移植瘤模型，在体验证miR-410对肿瘤生长的影响。［结果］ MiR-410在多发性骨髓瘤骨髓及细胞中表达均下调（P<0.01），而HMGB1表达均上调（P<0.01）。MiR-410抑制多发性骨髓瘤细胞的增殖和侵袭（P<0.01）。MiR-410通过与HMGB1的3’UTR作用，负向调控HMGB1的表达。MiR-410通过下调HMGB1抑制多发性骨髓瘤细胞增殖和侵袭（P<0.05）。MiR-410抑制多发性骨髓瘤移植瘤小鼠肿瘤生长（P<0.05）。［结论］ MiR-410通过负向调控HMGB1表达，抑制多发性骨髓瘤细胞增殖和侵袭，从而抑制肿瘤发生发展。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the expression of miR-410 and HMGB1 in multiple myeloma(MM) and its effect on cell proliferation and invasion. ［Methods］ The expression level of miR-410 in bone marrow of MM patients and multiple myeloma KM3 and U266 cells was determined by qRT-PCR. The expression level of HMGB1 was analyzed by Western blot. The CCK8 assay and Boyden chamber assay was used to detect the cell proliferation and cell invasion，respectively. Dual-luciferase reporter assay was performed to determine the interaction between miR-410 and HMGB1. The expression levels of miR-410 and HMGB1 in cells were modulated by recombinant plasmid construction and cell transfection. The nude mice model of MM xenograft was established to verify the effect of miR-410 on tumor growth. ［Results］ MiR-410 was down-regulated and HMGB1 was up-regulated in bone marrow of MM patients and MM cell lines(all P<0.01). MiR-410 inhibited the cell proliferation and invasion of multiple myeloma cells(P<0.01). MiR-410 negatively regulated the expression of HMGB1 through targeting 3’UTR of HMGB1. MiR-410 inhibited the growth of MM tumor in nude mice xenograft model(P<0.05). ［Conclusion］ MiR-410 inhibits cell proliferation and invasion of multiple myeloma through negatively regulating HMGB1，leading to the suppression of development and progression of multiple myeloma. |
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