| 刘 兰,张志敏,付红星.白藜芦醇通过自噬途径对HepG2细胞增殖和凋亡的影响[J].肿瘤学杂志,2019,25(2):127-132. |
| 白藜芦醇通过自噬途径对HepG2细胞增殖和凋亡的影响 |
| Effect of Resveratrol on Proliferation and Apoptosis of HepG2 Cells and Its Mechanism |
| 投稿时间：2018-05-10 |
| DOI：10.11735/j.issn.1671-170X.2019.02.B010 |
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| 中文关键词: 白藜芦醇 肝肿瘤 自噬 凋亡 增殖 |
| 英文关键词:resveratrol hepatocellular carcinoma autophagy apoptosis proliferation |
| 基金项目:湖北省卫生计生科研基金（WJ2018H0067）；国家自然科学基金（81301631） |
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| 中文摘要: |
| 摘 要：［目的］ 研究白藜芦醇（Resveratrol，Res）对人肝癌HepG2细胞的增殖抑制作用及诱导凋亡效应，并从自噬角度探讨其机制。［方法］ 不同浓度Res及联合自噬抑制剂3-MA处理HepG2细胞。CCK-8法检测各组的细胞存活率；流式细胞术检测细胞凋亡；MDC染色后荧光显微镜下观察自噬形态；RT-PCR和Western blot检测Beclin1、Bcl-2 mRNA及蛋白表达。［结果］ Res可以显著降低HepG2细胞的存活率和诱导凋亡。Res诱导HepG2细胞发生自噬，Beclin1表达显著上调，Bcl-2表达显著下调；联合3-MA抑制自噬后，减弱了Res对HepG2细胞增殖抑制和诱导凋亡作用。［结论］ Res可通过诱导HepG2细胞自噬发挥抑制增殖和促凋亡作用，其机制可能与作用于Beclin1/Bcl-2复合体有关。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effects of resveratrol(Res) on proliferation and apoptosis of human hepatocellular carcinoma HepG2 cells and its mechanism. ［Methods］ HepG2 cells were treated with different concentrations of Res and/or autophagy inhibitor 3-MA. The cell viability was examined by CCK-8 assay；cell apoptosis was detected by Flow cytometry；the autophagy was determined by MDC staining；the expression levels of Beclin1 and Bcl-2 mRNA and the protein were detected by RT-PCR and Western blot，respectively. ［Results］ Res significantly inhibited the viability and induced apoptosis of HepG2 cells. Res induced the autophagy of HepG2 cells，upregulated Beclin1 expression，and downregulated Bcl-2 expression. With the combined treatment of autophagy inhibitor 3-MA，the effect of Res inhibiting cell proliferation and inducing cell apoptosis was crippled. ［Conclusion］ Res can inhibit proliferation and promote apoptosis by inducing autophagy of HepG2 cells，which may be related to the involvement of Beclin1/Bcl-2 complex. |
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