刘 兰,张志敏,付红星.白藜芦醇通过自噬途径对HepG2细胞增殖和凋亡的影响[J].肿瘤学杂志,2019,25(2):127-132.
白藜芦醇通过自噬途径对HepG2细胞增殖和凋亡的影响
Effect of Resveratrol on Proliferation and Apoptosis of HepG2 Cells and Its Mechanism
投稿时间:2018-05-10  
DOI:10.11735/j.issn.1671-170X.2019.02.B010
中文关键词:  白藜芦醇  肝肿瘤  自噬  凋亡  增殖
英文关键词:resveratrol  hepatocellular carcinoma  autophagy  apoptosis  proliferation
基金项目:湖北省卫生计生科研基金(WJ2018H0067);国家自然科学基金(81301631)
作者单位
刘 兰 湖北中医药大学 
张志敏 解放军武汉总医院 
付红星 湖北中医药大学 
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中文摘要:
      摘 要:[目的] 研究白藜芦醇(Resveratrol,Res)对人肝癌HepG2细胞的增殖抑制作用及诱导凋亡效应,并从自噬角度探讨其机制。[方法] 不同浓度Res及联合自噬抑制剂3-MA处理HepG2细胞。CCK-8法检测各组的细胞存活率;流式细胞术检测细胞凋亡;MDC染色后荧光显微镜下观察自噬形态;RT-PCR和Western blot检测Beclin1、Bcl-2 mRNA及蛋白表达。[结果] Res可以显著降低HepG2细胞的存活率和诱导凋亡。Res诱导HepG2细胞发生自噬,Beclin1表达显著上调,Bcl-2表达显著下调;联合3-MA抑制自噬后,减弱了Res对HepG2细胞增殖抑制和诱导凋亡作用。[结论] Res可通过诱导HepG2细胞自噬发挥抑制增殖和促凋亡作用,其机制可能与作用于Beclin1/Bcl-2复合体有关。
英文摘要:
      Abstract:[Objective] To investigate the effects of resveratrol(Res) on proliferation and apoptosis of human hepatocellular carcinoma HepG2 cells and its mechanism. [Methods] HepG2 cells were treated with different concentrations of Res and/or autophagy inhibitor 3-MA. The cell viability was examined by CCK-8 assay;cell apoptosis was detected by Flow cytometry;the autophagy was determined by MDC staining;the expression levels of Beclin1 and Bcl-2 mRNA and the protein were detected by RT-PCR and Western blot,respectively. [Results] Res significantly inhibited the viability and induced apoptosis of HepG2 cells. Res induced the autophagy of HepG2 cells,upregulated Beclin1 expression,and downregulated Bcl-2 expression. With the combined treatment of autophagy inhibitor 3-MA,the effect of Res inhibiting cell proliferation and inducing cell apoptosis was crippled. [Conclusion] Res can inhibit proliferation and promote apoptosis by inducing autophagy of HepG2 cells,which may be related to the involvement of Beclin1/Bcl-2 complex.
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