| 谢彦良,董亚辉,郭 苹.RNA干扰STMN1基因表达通过Notch信号通路诱导胃癌细胞凋亡及增强化疗敏感性的研究[J].肿瘤学杂志,2019,25(2):121-126. |
| RNA干扰STMN1基因表达通过Notch信号通路诱导胃癌细胞凋亡及增强化疗敏感性的研究 |
| RNA Interference with STMN1 Gene Expression Induces Apoptosis and Enhances Chemosensitivity in Gastric Cancer Cells via Notch Signaling Pathway |
| 投稿时间：2017-09-05 |
| DOI：10.11735/j.issn.1671-170X.2019.02.B009 |
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| 中文关键词: STMN1 胃肿瘤 药物疗法 凋亡 信号通路 |
| 英文关键词:STMN1 gastric cancer drug therapy apoptosis signal pathway |
| 基金项目:苏州市科技发展计划(SZS201509)；江苏省科技厅临床医学科技专项（BL2014040） |
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| 中文摘要: |
| 摘 要：［目的］ 探讨RNA干扰微管不稳定性蛋白(STMN1)基因表达通过Notch信号通路对胃癌细胞凋亡及化疗敏感性的影响。［方法］ RT-PCR及Western blotting分别检测人胃黏膜上皮细胞GES-1和人胃癌细胞MKN28、SGC7901、BGC823中STMN1 mRNA及蛋白表达；STMN1-siRNA转染BGC823细胞，同时设定对照组和阴性对照组，转染48h后RT-PCR及Western blotting分别检测STMN1 mRNA及蛋白表达；后续实验分为对照组、STMN1-siRNA组、顺铂组、STMN1-siRNA+顺铂组，细胞培养48h后，CCK8及流式细胞仪分别检测细胞增殖及凋亡情况；Western blotting检测活化的含半胱氨酸的天冬氨酸蛋白水解酶3（Cleaved Caspase-3）、Notch1、Hes1蛋白表达。［结果］ STMN1在MKN28、BGC823、SGC7901胃癌细胞中的mRNA及蛋白表达均显著高于在GES-1细胞中的表达（P<0.05），选择BGC823细胞作为研究对象；转染STMN1-siRNA后BGC823细胞中STMN1的mRNA及蛋白表达均显著低于对照组（P<0.05）；STMN1-siRNA组、顺铂组、STMN1-siRNA+顺铂组OD值及Notch1、Hes1蛋白表达均显著低于对照组，细胞凋亡率及Cleaved caspase3蛋白表达均显著高于对照组（P<0.05），STMN1-siRNA+顺铂组OD值及Notch1、Hes1蛋白表达均显著低于STMN1-siRNA组和顺铂组，细胞凋亡率及Cleaved caspase3蛋白表达均显著高于STMN1-siRNA组和顺铂组（P<0.05）。［结论］ RNA干扰STMN1表达可通过抑制Notch信号通路增强顺铂对胃癌细胞增殖的抑制及凋亡的促进作用。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effect of microtubule instability protein(STMN1) gene expression on apoptosis and chemosensitivity of gastric cancer cells and its relation to Notch signaling pathway. ［Methods］ The expression of STMN1 mRNA and protein in human gastric epithelial GES-1 cells and human gastric cancer MKN28，SGC7901 and BGC823 cells were detected by RT-PCR and Western blotting，respectively. STMN1-siRNA was transfected into BGC823 cells，and mRNA and protein expression of STMN1 were detected by RT-PCR and Western blotting 48h after transfection. The gastric cancer BGC823 cells were divided into the control group，STMN1-siRNA group，cisplatin group and STMN1-siRNA+cisplatin group. The cell proliferation and apoptosis were detected by CCK8 and flow cytometry respectively；the expression of Cleaved caspase3，Notch1，Hes1 protein were detected by Western blotting. ［Results］ The expressions of STMN1 mRNA and protein in gastric cancer MKN28，BGC823 and SGC7901 cells were significantly higher than those in GES-1 cells(P<0.05). The mRNA and protein expressions of STMN1 in BGC823 cells were significantly lower than those in the control group after cells were transfected with STMN1-siRNA(P<0.05). The OD value，Notch1 and Hes1 protein expression in STMN1-siRNA group，cisplatin group and STMN1-siRNA+ cisplatin group were significantly lower than those in the control group，the apoptosis rate and the expression of Cleaved caspase3 protein were significantly higher than those in control group(P<0.05). The OD value，Notch1 and Hes1 protein expression in STMN1-siRNA+ cisplatin group were significantly lower，and the apoptosis rate and the expression of Cleaved caspase3 protein were significantly higher than those in STMN1-siRNA group and cisplatin group. ［Conclusion］ RNA interference with STMN1 gene expression can inhibit the proliferation and promote the apoptosis of gastric cancer cells via Notch signaling pathway. |
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