| 邹 珏,张倩倩,沈丽华.肺腺癌EGFR突变及与TTF-1、CK7、NapsinA蛋白表达的关系[J].肿瘤学杂志,2018,24(11):1076-7079. |
| 肺腺癌EGFR突变及与TTF-1、CK7、NapsinA蛋白表达的关系 |
| Relationship Between EGFR Mutation and TTF-1，CK7，Napsin A Protein Expression in Lung Adenocarcinoma |
| 投稿时间：2018-01-03 |
| DOI：10.11735/j.issn.1671-170X.2018.11.B008 |
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| 中文关键词: 肺腺癌 EGFR 病理分型 TTF-1 |
| 英文关键词:lung adenocarcinoma EGFR pathological typing TTF-1 |
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| 摘要点击次数: 1535 |
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| 中文摘要: |
| 摘 要：［目的］ 探讨新分类肺腺癌中EGFR突变情况与TTF-1、CK7、NapsinA表达的关系及临床意义。［方法］ 收集南京市胸科医院2013~2015年外科手术切除标本307例，根据2011年IASLC/ATS/ERS肺腺癌国际多学科新分类进行病理分型，用ARMS法检测EGFR基因，用免疫组化法检测TTF-1、CK7、NapsinA蛋白的表达。［结果］ EGFR在肺腺癌中突变率为51.1%(157/307)，19外显子缺失突变(del)和21外显子（L858R）突变率分别为43.9%和51.0%，乳头和实体型腺癌突变率分别为65.4%和 29%。EGFR突变与性别、吸烟及肺腺癌病理分型均有相关性（P<0.001），与TTF-1、CK7蛋白表达有相关性（均P<0.05），与NapsinA无相关性（P=0.108）。［结论］ EGFR基因突变多见于女性非吸烟患者，较少见于实体为主型肺腺癌，突变以21（L858R）和19外显子缺失突变为主，TTF-1蛋白表达及肺腺癌病理分型对EGFR突变有着较好的提示作用。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the relationship between EGFR mutation and protein expression of TTF-1，CK7，NapsinA in patients with lung adenocarcinoma and its clinical significance. ［Methods］ A total of 307 patients with lung adenocarcinoma underwent surgical resection from 2013 to 2015 in Nanjing Chest Hospital，the tumors were classified according to the 2011 international multidisciplinary IASLC/ATS/ERS classification. The mutations of EGFR gene were detected by the amplification refractory system method，the expression of TTF-1，NapsinA and CK7 proteins was detected by immunohistochemistry. ［Results］ There were 157 cases of EGFR mutations in 307 cases of lung adenocarcinoma(51.1%). The mutation rates of 19 exon deletion and 21exon (L858R) were 43.9% and 51.0%，respectively; the mutation rate of papillary and solid predominant adenocarcinoma were 65.4% and 29% respectively. EGFR mutation was associated with gender，smoking and pathological type of lung adenocarcinoma(P<0.001)，it was correlated with the expression of TTF-1 ，CK7 protein and was not correlated with the expression of Napsin A protein(all P<0.05). ［Conclusion］ EGFR mutations are more common in female non-smoking patients and less common in solid predominant lung adenocarcinoma，mutations in 21 exon (L858R) and 19 exon deletion are dominated. TTF-1 protein expression and pathological type of lung adenocarcinoma may indicate EGFR mutation. |
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