| 孙靖驰,许在华,王 振.染色体1p、19q缺失检测在胶质瘤病理诊断中的意义[J].肿瘤学杂志,2018,24(10):973-976. |
| 染色体1p、19q缺失检测在胶质瘤病理诊断中的意义 |
| Detection of Chromosome 1p and 19q Deletions in Pathological Diagnosis of Glioma Types |
| 投稿时间：2017-12-14 |
| DOI：10.11735/j.issn.1671-170X.2018.10.B007 |
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| 中文关键词: 1p 19q 共缺失 杂合性缺失 胶质瘤 |
| 英文关键词:1p 19q co-deletion loss of heterozygosity glioma |
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| 中文摘要: |
| 摘 要：［目的］ 研究染色体1p和19q的缺失在不同病理类型胶质瘤中的分布差异，评价其在胶质瘤病理鉴别诊断中的价值。［方法］ 选取经术后病理确诊的Ⅱ～Ⅲ级胶质瘤患者共134例。采用特异性扩增片段分析法检测肿瘤组织染色体1p、19q缺失情况。［结果］ 134例患者中，组织病理学认定为间变性少突胶质细胞瘤（anaplastic oligodendroglioma，AO，WHO Ⅲ级）9例（6.7%），少突胶质细胞瘤（oligodendroglial tumor，OT，WHO Ⅱ级）15例（11.2%），混合型胶质瘤（星形+少突）32例（23.9%），星型细胞瘤（astrocytoma，AA）78例（58.2%）。少突胶质细胞瘤、间变性少突胶质细胞瘤、混合型胶质瘤、星型细胞瘤的1p、19q缺失率为分别为73.3%（11/15）和 66.7%（10/15）、 66.7%（6/9）和77.8%（7/9）、53.1%（17/32）和59.4%（19/32）、43.6%（34/78）和41.0%（32/78）。染色体1p和19q的缺失情况（包括完整，杂合性缺失，联合缺失）在少突胶质细胞瘤、混合型胶质瘤、星型细胞瘤的分布差异有统计学意义（P<0.05），但在不同级别少突胶质细胞瘤中，染色体缺失差异无统计学意义。 ［结论］ 染色体1p、19q的缺失与胶质瘤病理类型相关。单纯依靠组织病理学诊断难以准确判断胶质瘤类型，检测染色体1p、19q缺失情况可作为胶质瘤病理诊断的重要参考指标。 |
| 英文摘要: |
| Abstract：［Objective］ To evaluate the chromosome 1p and 19q deletion in pathological diagnosis of glioma types. ［Methods］ One hundred and thirty four patients with pathologically confirmed grade Ⅱ～Ⅲ glioma were included in the study. The deletion of chromosome 1 p and 19q in tumor tissues were detected by specific amplification fragment analysis. ［Results］ Histopathology demonstrated that of 134 patients，there were 9 cases(6.7%) of anaplastic oligodendroglioma(AO，WHO Ⅲ)，15 cases(11.2%) of oligodendroglioma(OT，WHO Ⅱ)，32 cases(23.9%) of mixed glioma(oligodendrocytes with astrocytic ingredients），78 cases(58.2%) of astrocytoma(AA). Heterozygous deletion rates of 1p and 19q in OT，AO，mixed glioma，AA were 73.3%(11/15) and 66.7%(10/15)，66.7%(6/9) and 77.8%(7/9)，53.1%(17/32) and 59.4%(19/32)，43.6%(34/78) and 41.0%(32/78)，respectively. There were significant differences in distribution of chromosome 1P and 19q status(intact，loss of heterozygosity，co-deletion) among OT+AO，mixed glioma and AA(P<0.05)，while there was no significant difference between OT and AO. ［Conclusion］ The deletion of chromosome 1p and 19q is related to the pathological types of glioma，indicating that it might be of value for diagnosis of glioma types. |
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